FastQCFastQC Report
Thu 26 May 2016
SRR1546937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546937_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1350983
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35460.2624755455842153No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.2403435128347285No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.13020149032223202No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16660.12331761391520102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGA1250.045.0000042
AACGTAC207.0337276E-445.037
TCGTTAG406.8157533E-945.01
GGTACGA253.8911712E-545.02
CGAGTTA351.2121382E-745.06
ATTAGAC207.0337276E-445.030
CAAGTCG207.0337276E-445.01
CGACGTG207.0337276E-445.036
TTTACGT351.2121382E-745.013
CTATCGT351.2121382E-745.013
GCGCGTC253.8911712E-545.038
CGACACG207.0337276E-445.029
GCGCATC207.0337276E-445.013
CCGCTCG800.045.019
TAGCCGA502.1827873E-1145.02
GTAGCGT207.0337276E-445.023
ATATCGA253.8911712E-545.026
TACGCGT207.0337276E-445.034
TGCGCGT253.8911712E-545.037
TCTACGA207.0337276E-445.02