##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546937_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1350983
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812058330859827	31.0	31.0	34.0	30.0	34.0
2	32.16184215493459	33.0	31.0	34.0	30.0	34.0
3	32.18903198633883	33.0	31.0	34.0	30.0	34.0
4	35.906307481293254	37.0	35.0	37.0	35.0	37.0
5	35.874569850249785	37.0	35.0	37.0	35.0	37.0
6	35.92278437256427	37.0	35.0	37.0	35.0	37.0
7	36.15889467150956	37.0	35.0	37.0	35.0	37.0
8	13.258178674343052	0.0	0.0	37.0	0.0	37.0
9	25.018865522364084	18.0	17.0	39.0	17.0	39.0
10	32.835877283429916	32.0	32.0	38.0	27.0	39.0
11	35.68390497881913	37.0	35.0	37.0	32.0	39.0
12	36.561799075191914	37.0	35.0	39.0	33.0	39.0
13	36.601641175351574	39.0	35.0	39.0	33.0	39.0
14	37.764054766048126	39.0	36.0	41.0	33.0	41.0
15	38.17521019879599	40.0	37.0	41.0	33.0	41.0
16	38.3371470995564	40.0	37.0	41.0	34.0	41.0
17	38.354397501671	40.0	37.0	41.0	34.0	41.0
18	38.310613827116995	40.0	36.0	41.0	34.0	41.0
19	38.26576574242607	40.0	36.0	41.0	34.0	41.0
20	38.23141519915499	40.0	36.0	41.0	34.0	41.0
21	38.14651331659984	40.0	36.0	41.0	34.0	41.0
22	38.085123943084405	40.0	36.0	41.0	34.0	41.0
23	38.01367744819883	40.0	36.0	41.0	34.0	41.0
24	37.93221306263661	40.0	35.0	41.0	34.0	41.0
25	37.841401409196116	40.0	35.0	41.0	33.0	41.0
26	37.76136413263527	40.0	35.0	41.0	33.0	41.0
27	37.67322904877412	40.0	35.0	41.0	33.0	41.0
28	37.70031229112431	40.0	35.0	41.0	33.0	41.0
29	37.750799232854895	40.0	36.0	41.0	33.0	41.0
30	37.680545943213204	40.0	36.0	41.0	33.0	41.0
31	37.57815235276832	40.0	35.0	41.0	33.0	41.0
32	37.41622951584143	40.0	35.0	41.0	33.0	41.0
33	37.3056766813498	40.0	35.0	41.0	33.0	41.0
34	37.19754134582004	40.0	35.0	41.0	33.0	41.0
35	37.10024404452166	40.0	35.0	41.0	32.0	41.0
36	36.98732996640224	40.0	35.0	41.0	32.0	41.0
37	36.86506269879044	40.0	35.0	41.0	31.0	41.0
38	36.829445670300814	40.0	35.0	41.0	31.0	41.0
39	36.80104708941563	40.0	35.0	41.0	31.0	41.0
40	36.646180595906834	39.0	35.0	41.0	31.0	41.0
41	36.654071886914934	39.0	35.0	41.0	31.0	41.0
42	36.616866385439344	39.0	35.0	41.0	31.0	41.0
43	36.51929817029526	39.0	35.0	41.0	31.0	41.0
44	36.33016403611296	39.0	35.0	41.0	30.0	41.0
45	36.23483789211263	39.0	35.0	40.0	30.0	41.0
46	36.236329398667486	39.0	35.0	40.0	30.0	41.0
47	36.236581067267316	39.0	35.0	40.0	30.0	41.0
48	36.20744746603029	39.0	35.0	40.0	30.0	41.0
49	36.16262602860288	39.0	35.0	40.0	30.0	41.0
50	36.02232596561171	39.0	35.0	40.0	30.0	41.0
51	35.601598243649256	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	4.0
15	15.0
16	50.0
17	134.0
18	312.0
19	584.0
20	1154.0
21	1952.0
22	3382.0
23	5227.0
24	7682.0
25	10910.0
26	14073.0
27	15357.0
28	16439.0
29	18975.0
30	23722.0
31	31012.0
32	41938.0
33	59136.0
34	97719.0
35	168094.0
36	129457.0
37	230576.0
38	350554.0
39	122485.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.63081992889622	24.03405520276717	31.234960025403723	13.100164842932887
2	29.191188934279705	25.505872390696254	31.96857399389926	13.33436468112478
3	28.502505212870926	25.140138699006577	33.74157927968005	12.615776808442444
4	26.019868495754572	27.615299378304535	32.02919651838698	14.335635607553906
5	23.660993513611942	32.36857902727125	30.21185314693079	13.758574312186015
6	20.23933683843542	40.51642396684488	28.11123456031645	11.133004634403246
7	87.96816836333248	4.702945929001327	5.835232567693302	1.4936531399728938
8	33.77377805642262	63.61634454319558	1.8381430410301243	0.7717343593516721
9	87.76683348347093	4.282733387466756	5.857586660972048	2.092846468090272
10	55.70010873563916	22.429149737635484	11.76121387167714	10.109527655048213
11	49.5499943374565	20.97642975522268	18.204892289540282	11.268683617780535
12	43.111497331942736	22.142987735596968	21.29449445329808	13.45102047916221
13	20.36805792522926	44.42957461344814	21.42321554009192	13.779151921230689
14	13.767900854414897	45.90590703213882	26.047922142617637	14.27826997082865
15	12.17269203239419	25.313123851299384	49.38330090015937	13.130883216147057
16	14.049251544986133	19.059381206129167	48.88085194262252	18.01051530626218
17	15.17339596427194	18.997352298289467	28.10442470408584	37.72482703335275
18	20.957702650588498	23.72672343027262	34.46401620153621	20.851557717602663
19	28.456760743843557	25.232219798472666	25.474561856070725	20.83645760161305
20	31.21963784888485	24.33598350238308	23.804518635689718	20.639860013042355
21	21.96496921130762	29.010949804697766	28.179629203328243	20.844451780666372
22	21.971482986832548	24.30274844317064	26.03519067227345	27.69057789772336
23	17.883496683525994	31.199356320545856	25.339549054281218	25.577597941646935
24	19.074407301942365	25.446582229384084	39.090499288296	16.388511180377545
25	16.034398656385758	26.193075708576647	37.10194724878107	20.670578386256526
26	15.228615015881028	37.13577446940487	26.839715969779043	20.79589454493506
27	16.338621581470676	36.47988168615001	29.766917866472042	17.414578865907266
28	13.50535128865426	29.494597637424008	41.391416472302026	15.608634601619709
29	14.185596709951199	25.38877247160031	39.776444263177254	20.649186555271235
30	18.395790324526658	31.236884549990634	32.51765566258051	17.8496694629022
31	29.766991886648462	26.901300756560225	24.530656566366858	18.801050790424455
32	30.12317697558	27.88199407394468	25.92356824623256	16.07126070424276
33	27.44172206460037	28.727230468481096	24.901497650229498	18.929549816689033
34	18.71429914366058	29.442561453400966	27.318700531390842	24.52443887154761
35	18.41229682386825	26.21039642985885	31.724899573125644	23.652407173147257
36	30.807419486403603	23.377126137042435	27.998871932511367	17.8165824440426
37	19.99965950718847	31.498101752575717	29.55366573820692	18.948573002028894
38	19.342286320405215	33.431582780834404	24.056409296045917	23.169721602714468
39	19.475892738842752	31.696771906086163	28.34284369233366	20.48449166273743
40	23.161209282426203	26.91884353837169	25.67152954552352	24.24841763367859
41	16.795548130509413	23.80844170503996	29.031379373389598	30.364630791061025
42	22.513828819459608	24.651235433754533	24.96026967030673	27.874666076479127
43	22.27511375050611	26.1756069469416	25.1721894353963	26.377089867155988
44	19.166784482114135	32.32749782935833	28.086437801215858	20.419279887311685
45	17.278973902706397	38.6784289661676	22.318193493182374	21.72440363794363
46	22.55409579543192	32.839125288771214	25.678931563165484	18.92784735263138
47	21.21832769176222	28.32337638593528	26.80707307197796	23.65122285032454
48	23.2337490553175	24.149156577099788	30.938213138137193	21.678881229445523
49	21.182501926375092	23.246036404603167	32.22224113848953	23.349220530532214
50	19.159826585530684	33.153118877143534	27.47466104310713	20.212393494218652
51	17.574758527679474	34.347804524557304	24.84442809420992	23.2330088535533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1975.0
1	2242.0
2	2509.0
3	5616.5
4	8724.0
5	6637.5
6	4551.0
7	5073.0
8	5595.0
9	6108.0
10	6621.0
11	6712.0
12	6803.0
13	6684.5
14	6566.0
15	6273.0
16	5980.0
17	5628.0
18	5276.0
19	5697.0
20	6118.0
21	6019.5
22	5921.0
23	6509.0
24	7097.0
25	8371.0
26	11310.0
27	12975.0
28	14712.0
29	16449.0
30	20527.0
31	24605.0
32	27425.5
33	30246.0
34	33204.0
35	36162.0
36	40791.5
37	45421.0
38	63447.0
39	81473.0
40	106967.0
41	132461.0
42	140922.5
43	149384.0
44	143939.0
45	138494.0
46	133446.5
47	128399.0
48	119088.5
49	109778.0
50	101531.5
51	93285.0
52	82832.0
53	72379.0
54	63652.0
55	54925.0
56	49098.5
57	43272.0
58	38908.5
59	34545.0
60	29103.0
61	23661.0
62	19672.0
63	15683.0
64	13018.5
65	10354.0
66	8037.0
67	5720.0
68	4554.5
69	3389.0
70	2810.5
71	2232.0
72	1756.0
73	1280.0
74	877.0
75	373.5
76	273.0
77	233.5
78	194.0
79	121.5
80	49.0
81	33.5
82	18.0
83	17.0
84	16.0
85	10.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1350983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.784732093615634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62189791705619	14.956760211571133
2	5.985867838097698	2.248858473729109
3	1.8505433319141142	1.042858821529004
4	0.9982317761856461	0.7500606593192577
5	0.651835312696156	0.6122275859077732
6	0.5090514846011439	0.5737437456053875
7	0.41188877826012216	0.5416054246388136
8	0.3406095527482955	0.5118607357522379
9	0.3080500890976954	0.5207974555603171
>10	6.275817624959472	31.64654382042537
>50	2.5066539087647848	32.591335356767125
>100	0.5367711877547122	13.09765966781922
>500	0.0011919419417203085	0.14307408119624052
>1k	0.0015892559222937448	0.7626139601790222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3546	0.2624755455842153	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3247	0.2403435128347285	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	0.13020149032223202	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1666	0.12331761391520102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010732925580854829	0.0
2	0.0	0.0	0.0	0.039526774208113645	0.0
3	0.0	0.0	0.0	0.05803181831303577	0.0
4	0.0	0.0	0.0	0.09518994687571938	0.0
5	0.0	0.0	0.0	0.16691549782639753	0.0
6	0.0	0.0	0.0	0.2435263804207751	0.0
7	0.0	0.0	0.0	0.28890074856604414	0.0
8	0.0	0.0	0.0	0.4021516184881675	0.0
9	0.0	0.0	0.0	0.5159946498216483	0.0
10	0.0	0.0	0.0	0.7309492421444237	0.0
11	0.0	0.0	0.0	0.9735133602717428	0.0
12	0.0	0.0	0.0	1.1908365982399483	0.0
13	0.0	0.0	0.0	1.2684097431277819	0.0
14	0.0	0.0	0.0	1.2960932891087453	0.0
15	0.0	0.0	0.0	1.336508305433895	0.0
16	0.0	0.0	0.0	1.4302918689576405	0.0
17	0.0	0.0	0.0	1.5398417300587794	0.0
18	0.0	0.0	0.0	1.7154175885262806	0.0
19	0.0	0.0	0.0	1.7938789755311504	0.0
20	0.0	0.0	0.0	1.872562423065279	0.0
21	0.0	0.0	0.0	1.9787813762275321	0.0
22	0.0	0.0	0.0	2.0804110784517644	0.0
23	0.0	0.0	0.0	2.204690954660421	0.0
24	0.0	0.0	0.0	2.2942553681282445	0.0
25	0.0	0.0	0.0	2.3662029796081816	0.0
26	0.0	0.0	0.0	2.4434800437903363	0.0
27	0.0	0.0	0.0	2.5163899175637297	0.0
28	0.0	0.0	0.0	2.595443465979957	0.0
29	0.0	0.0	0.0	2.6791602855106245	0.0
30	0.0	0.0	0.0	2.7838248149680638	0.0
31	0.0	0.0	0.0	2.8924864339521665	0.0
32	0.0	0.0	0.0	2.9816067263614716	0.0
33	0.0	0.0	0.0	3.073243704769046	0.0
34	0.0	0.0	0.0	3.169469934114641	0.0
35	0.0	0.0	0.0	3.300115545495391	0.0
36	0.0	0.0	0.0	3.411737971536281	0.0
37	0.0	0.0	0.0	3.514551996583229	0.0
38	0.0	0.0	0.0	3.619734667275606	0.0
39	0.0	0.0	0.0	3.729284528376745	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGA	125	0.0	45.000004	2
AACGTAC	20	7.0337276E-4	45.0	37
TCGTTAG	40	6.8157533E-9	45.0	1
GGTACGA	25	3.8911712E-5	45.0	2
CGAGTTA	35	1.2121382E-7	45.0	6
ATTAGAC	20	7.0337276E-4	45.0	30
CAAGTCG	20	7.0337276E-4	45.0	1
CGACGTG	20	7.0337276E-4	45.0	36
TTTACGT	35	1.2121382E-7	45.0	13
CTATCGT	35	1.2121382E-7	45.0	13
GCGCGTC	25	3.8911712E-5	45.0	38
CGACACG	20	7.0337276E-4	45.0	29
GCGCATC	20	7.0337276E-4	45.0	13
CCGCTCG	80	0.0	45.0	19
TAGCCGA	50	2.1827873E-11	45.0	2
GTAGCGT	20	7.0337276E-4	45.0	23
ATATCGA	25	3.8911712E-5	45.0	26
TACGCGT	20	7.0337276E-4	45.0	34
TGCGCGT	25	3.8911712E-5	45.0	37
TCTACGA	20	7.0337276E-4	45.0	2
>>END_MODULE