FastQCFastQC Report
Thu 26 May 2016
SRR1546936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546936_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences855474
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.3150300301353402No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14680.17160077337242277No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11510.13454529301884102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA453.8380676E-1045.0000042
CATGACG253.8899667E-545.01
CACGAAC207.032274E-445.032
CGCGACA253.8899667E-545.027
CGTTACC207.032274E-445.029
CCCAACG207.032274E-445.015
ATATACG207.032274E-445.01
TAACACG351.211447E-745.01
AACGCGA253.8899667E-545.02
TTGCGTC551.8189894E-1245.016
CGTAAGA351.211447E-745.02
CCAACGG207.032274E-445.01
TAATGCG351.211447E-745.01
TAGTACG253.8899667E-545.01
TCACGAC1550.043.54838625
CGTTTTT26100.041.9827581
CGTTAGA1400.041.7857132
CGACGGT1850.041.35135328
CGCATCG603.6379788E-1241.25000421
CTTGCGT603.6379788E-1241.25000415