##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546936_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	855474
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74746164114865	31.0	31.0	34.0	30.0	34.0
2	32.10599270112242	33.0	31.0	34.0	30.0	34.0
3	32.14889523235072	33.0	31.0	34.0	30.0	34.0
4	35.886060827097026	37.0	35.0	37.0	35.0	37.0
5	35.877135950362025	37.0	35.0	37.0	35.0	37.0
6	35.91722016098677	37.0	35.0	37.0	35.0	37.0
7	36.11239616867374	37.0	35.0	37.0	35.0	37.0
8	13.269476337094991	0.0	0.0	36.0	0.0	37.0
9	24.95035734575218	18.0	17.0	39.0	17.0	39.0
10	32.73263360429423	32.0	32.0	38.0	27.0	39.0
11	35.59450550221281	37.0	35.0	37.0	32.0	39.0
12	36.612968950546716	37.0	35.0	39.0	33.0	39.0
13	36.80045682276726	39.0	35.0	39.0	33.0	39.0
14	37.96929421583824	40.0	37.0	41.0	33.0	41.0
15	38.287497925126885	40.0	37.0	41.0	33.0	41.0
16	38.39352803241244	40.0	37.0	41.0	34.0	41.0
17	38.3692245468594	40.0	37.0	41.0	34.0	41.0
18	38.31486053345864	40.0	37.0	41.0	34.0	41.0
19	38.30543418034914	40.0	37.0	41.0	34.0	41.0
20	38.280605839569645	40.0	37.0	41.0	34.0	41.0
21	38.200762384362356	40.0	37.0	41.0	34.0	41.0
22	38.13928886208114	40.0	37.0	41.0	34.0	41.0
23	38.052602416905714	40.0	36.0	41.0	34.0	41.0
24	37.99510797522777	40.0	36.0	41.0	34.0	41.0
25	37.872323413686445	40.0	36.0	41.0	33.0	41.0
26	37.81249926941088	40.0	36.0	41.0	33.0	41.0
27	37.75231509081515	40.0	35.0	41.0	33.0	41.0
28	37.71297666556786	40.0	36.0	41.0	33.0	41.0
29	37.731908859883525	40.0	36.0	41.0	33.0	41.0
30	37.63652665072229	40.0	36.0	41.0	33.0	41.0
31	37.56028353871655	40.0	36.0	41.0	33.0	41.0
32	37.43243979361149	40.0	35.0	41.0	33.0	41.0
33	37.296204209596084	40.0	35.0	41.0	33.0	41.0
34	37.16315165627476	40.0	35.0	41.0	32.0	41.0
35	37.055376317690545	40.0	35.0	41.0	32.0	41.0
36	36.942075387446025	40.0	35.0	41.0	31.0	41.0
37	36.883624750722994	40.0	35.0	41.0	31.0	41.0
38	36.80724837926109	40.0	35.0	41.0	31.0	41.0
39	36.74258247474499	40.0	35.0	41.0	31.0	41.0
40	36.6247472161632	39.0	35.0	41.0	31.0	41.0
41	36.5869214026376	39.0	35.0	41.0	31.0	41.0
42	36.53698066802732	39.0	35.0	41.0	31.0	41.0
43	36.448849409800886	39.0	35.0	41.0	30.0	41.0
44	36.27606449757678	39.0	35.0	41.0	30.0	41.0
45	36.18382440611871	39.0	35.0	40.0	30.0	41.0
46	36.156579860989346	39.0	35.0	40.0	30.0	41.0
47	36.12958430063333	39.0	35.0	40.0	30.0	41.0
48	36.073273997807064	39.0	35.0	40.0	30.0	41.0
49	35.973904525444375	38.0	35.0	40.0	29.0	41.0
50	35.85724171628828	38.0	35.0	40.0	29.0	41.0
51	35.474896957709994	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	4.0
14	5.0
15	15.0
16	36.0
17	83.0
18	226.0
19	372.0
20	800.0
21	1343.0
22	2158.0
23	3275.0
24	4931.0
25	7321.0
26	9377.0
27	10395.0
28	10973.0
29	12598.0
30	15519.0
31	20179.0
32	27237.0
33	37787.0
34	59859.0
35	92886.0
36	89298.0
37	156046.0
38	208373.0
39	84352.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.43743000956195	22.722607583631998	28.780886385793142	13.059076021012912
2	32.73962738785749	24.359244114958493	29.570857793457193	13.330270703726823
3	30.674924077178268	24.144976936762543	31.432281986360778	13.747816999698411
4	27.11128567320573	27.251558784954305	30.151705370356087	15.48545017148388
5	23.65331968008379	31.226548089129537	29.514397865978392	15.605734364808283
6	22.38887447193018	40.24365439510728	27.23297259764762	10.134498535314924
7	86.30291510905066	4.839305461066029	6.91745161162116	1.9403278182621564
8	32.664581273072	63.674758087329366	2.592948470672399	1.0677121689262328
9	82.79632110385587	5.506654790209872	8.26524242700538	3.431781678928875
10	48.22075247172912	25.248575643444454	14.687880636933443	11.84279124789298
11	41.49033167577273	22.483558822360468	21.444953324122064	14.581156177744736
12	37.729960232572815	22.409097178873935	25.027762386700235	14.833180201853008
13	23.50486397014988	33.66344272298164	25.39060216908988	17.44109113777859
14	17.556816454971162	36.09016755623198	29.01537627093284	17.337639717864015
15	17.181001409744773	24.95470347433119	41.65199643706296	16.212298678861075
16	20.365785517736366	20.961127982849273	40.51122535576768	18.16186114364668
17	21.273352550749642	20.585196043363094	28.445633648714047	29.695817757173216
18	24.326396827957367	24.381570918578472	31.174530143522773	20.11750210994139
19	28.142877515856707	25.998452320000375	25.78032763123134	20.078342532911577
20	30.623139920091084	24.818872344454654	24.439550471434547	20.11843726401971
21	25.564423933398327	26.792631921016884	27.89576305065963	19.747181094925153
22	24.422133226725766	23.80703563170827	27.653791932893345	24.11703920867262
23	22.507522145617518	28.496599545982694	26.559310978475093	22.4365673299247
24	22.963409758800385	24.792454241742004	33.36208932124179	18.88204667821582
25	20.56707743309557	25.17563362533519	32.342771375868814	21.91451756570042
26	19.772430255039897	31.726621732513205	27.055760899805254	21.445187112641648
27	19.19263472647912	30.340723388437286	30.315357334062753	20.151284551020836
28	17.889848201114237	27.76986793286529	35.3815545533821	18.958729312638372
29	19.887571100933517	25.960344791308675	34.13476037845686	20.017323729300948
30	21.652674423769746	27.64829790268319	31.30603618578706	19.392991487760003
31	28.569307775572373	25.43245031409488	25.749818229426026	20.248423680906726
32	28.9434863011617	26.742601177826565	26.24603436223661	18.067878158775137
33	27.42152303868966	26.02218185473784	25.81937031400136	20.736924792571134
34	21.1965530220673	27.124728513081635	28.967098941639374	22.71161952321169
35	21.286561602105966	27.03530440434192	29.995885322055376	21.682248671496737
36	27.794649515940872	26.291856912074472	26.50285105099629	19.410642520988365
37	22.222066363209166	30.429563025878053	27.110350519127408	20.238020091785373
38	22.129486109455108	31.509432197822495	24.448083752399256	21.91299794032314
39	21.64893380745645	29.05430205944307	26.803619981437194	22.493144151663287
40	23.669100405155504	26.060055594909958	26.54738776397646	23.723456235958075
41	19.230274678131657	25.76875509951208	28.039659884461717	26.961310337894545
42	23.001049710452918	26.878081624923727	24.834302386746995	25.28656627787636
43	22.401382157727763	27.037291606758355	25.659108283828612	24.902217951685266
44	20.868547729095216	29.548881672616584	27.952924343697177	21.629646254591023
45	21.139274834769964	32.41606407675744	23.928021190591416	22.516639897881173
46	23.08217432674751	30.366790808370563	26.08366823538763	20.46736662949429
47	22.687305517175275	27.44759045862294	26.94529582430325	22.919808199898537
48	22.72845229662152	25.677694471135297	29.85549531604701	21.738357916196165
49	21.630932091448717	25.192933975784186	30.37216794432093	22.80396598844617
50	20.13071116129771	30.275613285733993	27.554314917811645	22.039360635156648
51	19.647937868363037	31.373951750725325	26.378475558579222	22.599634822332415
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	604.0
1	1096.5
2	1589.0
3	4643.0
4	7697.0
5	5560.0
6	3423.0
7	3567.5
8	3712.0
9	3727.0
10	3742.0
11	3740.5
12	3739.0
13	3700.5
14	3662.0
15	3328.5
16	2995.0
17	2965.0
18	2935.0
19	3062.0
20	3189.0
21	3438.0
22	3687.0
23	4238.5
24	4790.0
25	5502.5
26	7207.0
27	8199.0
28	9689.5
29	11180.0
30	13144.5
31	15109.0
32	16734.0
33	18359.0
34	20521.5
35	22684.0
36	26107.5
37	29531.0
38	37404.5
39	45278.0
40	53535.0
41	61792.0
42	66655.5
43	71519.0
44	70556.0
45	69593.0
46	69730.0
47	69867.0
48	68521.5
49	67176.0
50	62937.5
51	58699.0
52	54170.5
53	49642.0
54	46399.5
55	43157.0
56	41790.5
57	40424.0
58	38525.0
59	36626.0
60	32616.5
61	28607.0
62	24626.0
63	20645.0
64	17181.0
65	13717.0
66	11069.5
67	8422.0
68	6936.5
69	5451.0
70	4556.0
71	3661.0
72	2897.5
73	2134.0
74	1629.0
75	827.0
76	530.0
77	376.5
78	223.0
79	147.0
80	71.0
81	56.5
82	42.0
83	28.0
84	14.0
85	11.5
86	9.0
87	5.5
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	855474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.29004267519012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70189366902756	17.41642057112624
2	5.385873288460669	2.6164618403975233
3	2.65648346539829	1.9357829022138415
4	1.8844723545720525	1.8309565565108459
5	1.5050168491390847	1.8278461746234265
6	1.266494731065163	1.8457926639285744
7	1.1694416431245083	1.9884051192347127
8	1.0466432774184626	2.033840789935625
9	0.9943361750986023	2.1737221313967323
>10	12.070503285903483	60.14056889161073
>50	0.28928302841397235	4.321006769267215
>100	0.027619987632007813	1.181709194706617
>500	4.8456118652645276E-4	0.06202796271019792
>1k	0.0014536835595793584	0.6254584323377453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2695	0.3150300301353402	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1468	0.17160077337242277	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1151	0.13454529301884102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0070136555874287234	0.0
2	0.0	0.0	0.0	0.023378851958095744	0.0
3	0.0	0.0	0.0	0.0368216918340008	0.0
4	0.0	0.0	0.0	0.05645992747880123	0.0
5	0.0	0.0	0.0	0.10146421749813554	0.0
6	0.0	0.0	0.0	0.14822192141432702	0.0
7	0.0	0.0	0.0	0.1769779093227848	0.0
8	0.0	0.0	0.0	0.24103596368796715	0.0
9	0.0	0.0	0.0	0.3142117703168068	0.0
10	0.0	0.0	0.0	0.44104204218947624	0.0
11	0.0	0.0	0.0	0.5944073110345843	0.0
12	0.0	0.0	0.0	0.7206531116083014	0.0
13	0.0	0.0	0.0	0.7769961448273122	0.0
14	0.0	0.0	0.0	0.799439842707084	0.0
15	0.0	0.0	0.0	0.835326380462761	0.0
16	0.0	0.0	0.0	0.9059305133762101	0.0
17	0.0	0.0	0.0	0.9910295345036787	0.0
18	0.0	0.0	0.0	1.1083913713333193	0.0
19	0.0	0.0	0.0	1.1694101749439492	0.0
20	0.0	0.0	0.0	1.239429836558446	0.0
21	0.0	0.0	0.0	1.3233599150880098	0.0
22	0.0	0.0	0.0	1.414303649205002	0.0
23	0.0	0.0	0.0	1.5077021627775946	0.0
24	0.0	0.0	0.0	1.5898788274103013	0.0
25	0.0	0.0	0.0	1.6501962654621882	0.0
26	0.0	0.0	0.0	1.7154232624252754	0.0
27	0.0	0.0	0.0	1.7813516249471053	0.0
28	0.0	0.0	0.0	1.8589694134479833	0.0
29	0.0	0.0	0.0	1.9418474436394326	0.0
30	0.0	0.0	0.0	2.0338432260945396	0.0
31	1.1689425979047873E-4	0.0	0.0	2.1307485674608464	0.0
32	1.1689425979047873E-4	0.0	0.0	2.215380011549153	0.0
33	1.1689425979047873E-4	0.0	0.0	2.3111164103175548	0.0
34	1.1689425979047873E-4	0.0	0.0	2.4065021263065858	0.0
35	1.1689425979047873E-4	0.0	0.0	2.5212922894208356	0.0
36	1.1689425979047873E-4	0.0	0.0	2.620184833203581	0.0
37	1.1689425979047873E-4	0.0	0.0	2.721532156441926	0.0
38	1.1689425979047873E-4	0.0	0.0	2.8397122530900996	0.0
39	1.1689425979047873E-4	0.0	0.0	3.0003249660422173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGA	45	3.8380676E-10	45.000004	2
CATGACG	25	3.8899667E-5	45.0	1
CACGAAC	20	7.032274E-4	45.0	32
CGCGACA	25	3.8899667E-5	45.0	27
CGTTACC	20	7.032274E-4	45.0	29
CCCAACG	20	7.032274E-4	45.0	15
ATATACG	20	7.032274E-4	45.0	1
TAACACG	35	1.211447E-7	45.0	1
AACGCGA	25	3.8899667E-5	45.0	2
TTGCGTC	55	1.8189894E-12	45.0	16
CGTAAGA	35	1.211447E-7	45.0	2
CCAACGG	20	7.032274E-4	45.0	1
TAATGCG	35	1.211447E-7	45.0	1
TAGTACG	25	3.8899667E-5	45.0	1
TCACGAC	155	0.0	43.548386	25
CGTTTTT	2610	0.0	41.982758	1
CGTTAGA	140	0.0	41.785713	2
CGACGGT	185	0.0	41.351353	28
CGCATCG	60	3.6379788E-12	41.250004	21
CTTGCGT	60	3.6379788E-12	41.250004	15
>>END_MODULE