FastQCFastQC Report
Thu 26 May 2016
SRR1546935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546935_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1097801
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.38212754406308613No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22670.2065037288178823No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11170.10174885976602316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG700.045.01
TATATCG207.0331484E-445.027
CAAATCG351.2118653E-745.01
TATACGC207.0331484E-445.012
ATTACCG302.1654123E-644.9999961
ACAATCG302.1654123E-644.99999614
TAATCGC302.1654123E-644.99999616
CGGTCTA2000.043.87531
AGCGATA1200.043.1249968
CGTTTTT38400.042.6562461
GCGTAAG750.042.0000041
CGACGGT2150.041.86046228
TATACGA603.6379788E-1241.2499962
TCTAGCG556.184564E-1140.9090921
AGGGGAA97050.040.4791378
GAGGGGA95550.040.1491367
TACGTAG451.9281288E-840.0000041
GCGATAA750.039.0000049
GAGACCG1800.038.7500047
GAGCGAT6900.038.478267
Produced by FastQC (version 0.11.5)