##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546935_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1097801
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.743582853358667	31.0	31.0	34.0	30.0	34.0
2	32.1006238835636	33.0	31.0	34.0	30.0	34.0
3	32.12429301849789	33.0	31.0	34.0	30.0	34.0
4	35.873699331663936	37.0	35.0	37.0	35.0	37.0
5	35.851054061710634	37.0	35.0	37.0	35.0	37.0
6	35.89116242379083	37.0	35.0	37.0	35.0	37.0
7	36.12103650843823	37.0	35.0	37.0	35.0	37.0
8	13.185858821407523	0.0	0.0	36.0	0.0	37.0
9	24.930835369980535	18.0	17.0	39.0	17.0	39.0
10	32.65333061274311	32.0	32.0	37.0	27.0	39.0
11	35.5441049880625	37.0	35.0	37.0	32.0	39.0
12	36.603409907624425	37.0	35.0	39.0	33.0	39.0
13	36.79568063792983	39.0	35.0	39.0	33.0	39.0
14	37.98211788839689	40.0	37.0	41.0	33.0	41.0
15	38.29290554481186	40.0	37.0	41.0	33.0	41.0
16	38.39908599099473	40.0	37.0	41.0	34.0	41.0
17	38.39252013798494	40.0	37.0	41.0	34.0	41.0
18	38.33879546475181	40.0	37.0	41.0	34.0	41.0
19	38.30373628735991	40.0	37.0	41.0	34.0	41.0
20	38.28307953809479	40.0	37.0	41.0	34.0	41.0
21	38.1930869073721	40.0	37.0	41.0	34.0	41.0
22	38.14975756079654	40.0	37.0	41.0	34.0	41.0
23	38.044018906887494	40.0	36.0	41.0	34.0	41.0
24	37.95535165298629	40.0	36.0	41.0	34.0	41.0
25	37.86108411269438	40.0	36.0	41.0	33.0	41.0
26	37.80272107604201	40.0	36.0	41.0	33.0	41.0
27	37.72785504841041	40.0	36.0	41.0	33.0	41.0
28	37.687046195075425	40.0	36.0	41.0	33.0	41.0
29	37.71234677323121	40.0	36.0	41.0	33.0	41.0
30	37.64211910901885	40.0	36.0	41.0	33.0	41.0
31	37.56191604853703	40.0	36.0	41.0	33.0	41.0
32	37.43264034191989	40.0	35.0	41.0	33.0	41.0
33	37.295010662223845	40.0	35.0	41.0	32.0	41.0
34	37.142183328308136	40.0	35.0	41.0	32.0	41.0
35	37.010817078869486	40.0	35.0	41.0	31.0	41.0
36	36.90743677588197	40.0	35.0	41.0	31.0	41.0
37	36.83501563580285	40.0	35.0	41.0	31.0	41.0
38	36.76281493640469	40.0	35.0	41.0	31.0	41.0
39	36.68812653659452	40.0	35.0	41.0	31.0	41.0
40	36.59087029434296	39.0	35.0	41.0	30.0	41.0
41	36.537217583150316	39.0	35.0	41.0	30.0	41.0
42	36.4880884604769	39.0	35.0	41.0	30.0	41.0
43	36.39913609114949	39.0	35.0	41.0	30.0	41.0
44	36.237221500071506	39.0	35.0	41.0	30.0	41.0
45	36.14639538495592	39.0	35.0	40.0	30.0	41.0
46	36.14292025603912	39.0	35.0	40.0	30.0	41.0
47	36.08123967822948	39.0	35.0	40.0	30.0	41.0
48	36.013785740767226	39.0	35.0	40.0	29.0	41.0
49	35.92780203333756	38.0	35.0	40.0	29.0	41.0
50	35.825747107171516	38.0	35.0	40.0	29.0	41.0
51	35.42598430863153	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	1.0
14	10.0
15	18.0
16	50.0
17	128.0
18	282.0
19	547.0
20	1043.0
21	1679.0
22	2741.0
23	4158.0
24	6461.0
25	9496.0
26	12204.0
27	13795.0
28	14769.0
29	16355.0
30	20036.0
31	26580.0
32	35327.0
33	49104.0
34	76285.0
35	116239.0
36	116631.0
37	202150.0
38	263625.0
39	108058.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.423726158019534	22.588975597580983	29.60363490286491	14.383663341534577
2	32.35577304083345	24.988135372439995	29.523839020004537	13.13225256672202
3	29.15801679903735	24.810507551004235	32.62412768798716	13.40734796197125
4	26.739363509415643	26.83045469989552	31.676414942234523	14.753766848454319
5	23.71158342905499	31.190443441024374	30.436481657422433	14.661491472498204
6	21.455619005630346	39.94266720471196	27.942951409226264	10.658762380431426
7	86.94645022185259	4.006008374924052	7.095366100049098	1.9521753031742546
8	32.870620449425715	63.740240717579965	2.4841478555767393	0.9049909774175829
9	84.47095602937145	4.4876074989911645	8.086073887708247	2.9553625839291455
10	47.04932861238057	24.20475113431305	15.222339932282807	13.523580321023573
11	40.848204729272425	22.41198541447858	21.932845752554424	14.806964103694567
12	36.416253947664465	22.16558374423051	24.93994813267614	16.478214175428878
13	22.864526448782613	32.57721572489003	26.94586723823352	17.612390588093835
14	18.064567257635947	35.067739963800356	28.833458887357544	18.034233891206146
15	17.26797479688942	24.200014392408097	41.414518660485825	17.11749215021666
16	19.875551215566393	21.442866238963163	39.3929318701659	19.28865067530454
17	21.278811004908903	20.839933649176853	27.944864324226337	29.936391021687903
18	24.154195523596716	24.117485773833327	31.64808558199528	20.080233120574675
19	27.418903790395525	25.613567486274835	26.372357102972217	20.595171620357426
20	30.049526280263912	24.593163970519246	25.379189853170107	19.978119896046735
21	24.88019231172134	26.92619154108987	28.367163083291054	19.826453063897738
22	24.39777336693991	22.585149767580827	28.618574769015513	24.398502096463748
23	21.612113670874777	26.959348734424545	28.196367101141284	23.232170493559398
24	22.1167588661333	24.888845974816928	34.28790828210213	18.706486876947643
25	20.9823091798969	25.057911224347585	32.52338083131642	21.436398764439094
26	19.909437138424906	30.64234774790695	27.33127406515388	22.116941048514256
27	19.48422346126484	29.730798204774818	30.70793340505246	20.07704492890788
28	17.819987411197474	26.763684857273766	36.08723256765115	19.329095163877604
29	18.789379860284335	25.358785426502617	34.88328030307861	20.96855441013444
30	21.209763882525156	26.982212623234997	31.667670187948456	20.140353306291395
31	27.851131489222546	24.853047136958338	27.06055104704769	20.235270326771428
32	28.150183867567986	25.83355271128374	27.52229229159019	18.49397112955809
33	26.435392206784293	26.111289751056887	26.935847207280737	20.517470834878086
34	20.91052932179876	27.26076948372246	29.21759043761119	22.611110756867593
35	21.938584497554654	26.079954381531806	30.140799653124745	21.840661467788788
36	28.401777735673406	24.773251254097964	27.15856516800404	19.666405842224595
37	22.25549074923415	29.50552969071808	28.134060726853043	20.104918833194724
38	22.15774990184924	30.166487368840073	25.651916877466864	22.023845851843824
39	22.059189233750015	28.95779836236258	27.23826995967393	21.74474244421348
40	23.52184047928541	26.3669827227339	26.887113420374003	23.224063377606686
41	20.21878282129457	25.375364023169954	27.862882252794453	26.542970902741025
42	22.93193392973772	26.575672640123297	26.299757424159754	24.192636005979224
43	22.804588445446853	26.644719762507048	26.438398216070127	24.112293575975976
44	21.477571982536	28.579952104252044	28.542422533774335	21.400053379437622
45	20.583967403928398	31.727152735331813	25.160844269589845	22.528035591149944
46	23.22934666665452	28.945318869266835	27.19245109086255	20.632883373216092
47	22.41918161852649	27.329907697296683	27.73763186588462	22.513278818292203
48	23.529309956904758	25.45488663245889	29.41589595928588	21.59990745135047
49	22.089886964941734	24.73198694481058	30.33017823813241	22.847947852115276
50	21.54261109253863	29.032219864984636	27.981209709227812	21.443959333248923
51	20.878738496321283	30.60827964266748	26.53012704488336	21.98285481612788
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	645.0
1	1276.5
2	1908.0
3	6630.5
4	11353.0
5	8095.0
6	4837.0
7	4809.5
8	4782.0
9	4915.0
10	5048.0
11	4949.0
12	4850.0
13	4646.5
14	4443.0
15	4248.0
16	4053.0
17	3965.5
18	3878.0
19	3792.0
20	3706.0
21	3858.0
22	4010.0
23	4760.5
24	5511.0
25	6231.5
26	8309.5
27	9667.0
28	11249.0
29	12831.0
30	15475.0
31	18119.0
32	20279.0
33	22439.0
34	25364.5
35	28290.0
36	33156.5
37	38023.0
38	47692.0
39	57361.0
40	69890.5
41	82420.0
42	88092.0
43	93764.0
44	92578.5
45	91393.0
46	91889.0
47	92385.0
48	88563.5
49	84742.0
50	80821.5
51	76901.0
52	72364.0
53	67827.0
54	63062.0
55	58297.0
56	55041.0
57	51785.0
58	48253.0
59	44721.0
60	39393.0
61	34065.0
62	28774.0
63	23483.0
64	19913.0
65	16343.0
66	13547.0
67	10751.0
68	8747.5
69	6744.0
70	5545.0
71	4346.0
72	3486.0
73	2626.0
74	1993.5
75	959.0
76	557.0
77	428.0
78	299.0
79	226.0
80	153.0
81	112.5
82	72.0
83	50.5
84	29.0
85	23.0
86	17.0
87	14.0
88	11.0
89	6.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1097801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.783878887302258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14334570243928	17.39632475598896
2	5.735944283526448	2.728460082874135
3	2.498728486919427	1.7828836711543103
4	1.672282023753587	1.5909341247347213
5	1.3074405787014753	1.5548004188090134
6	1.149058471973538	1.6397440519108333
7	0.962001216375495	1.6016084293798558
8	0.8732256394162178	1.6614954279329919
9	0.8223844326089592	1.7603542569576844
>10	11.272411774565091	58.65115935658228
>50	0.5311611473797699	7.692871155577009
>100	0.030473290902590044	1.1973434440110968
>500	3.857378595264335E-4	0.04669740349410969
>1k	0.0011572135785793006	0.69532342059304
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4195	0.38212754406308613	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2267	0.2065037288178823	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1117	0.10174885976602316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009928939762306648	0.0
2	0.0	0.0	0.0	0.03261064619179615	0.0
3	0.0	0.0	0.0	0.04882487809721434	0.0
4	0.0	0.0	0.0	0.0793404269079733	0.0
5	0.0	0.0	0.0	0.1465657254821229	0.0
6	0.0	0.0	0.0	0.2185277659612261	0.0
7	0.0	0.0	0.0	0.2627069933439667	0.0
8	0.0	0.0	0.0	0.363636032395671	0.0
9	0.0	0.0	0.0	0.4753138319240008	0.0
10	0.0	0.0	0.0	0.6930217771709081	0.0
11	0.0	0.0	0.0	0.89232930194088	0.0
12	0.0	0.0	0.0	1.0716878559957588	0.0
13	0.0	0.0	0.0	1.1416458902843047	0.0
14	0.0	0.0	0.0	1.171068344809305	0.0
15	0.0	0.0	0.0	1.2126059276681294	0.0
16	0.0	0.0	0.0	1.2993247410049726	0.0
17	0.0	0.0	0.0	1.4056281602949896	0.0
18	0.0	0.0	0.0	1.545999684824481	0.0
19	0.0	0.0	0.0	1.6246113822086152	0.0
20	0.0	0.0	0.0	1.6988507024497155	0.0
21	0.0	0.0	0.0	1.812805781740042	0.0
22	0.0	0.0	0.0	1.9248479460302914	0.0
23	0.0	0.0	0.0	2.0542885277021976	0.0
24	0.0	0.0	0.0	2.1560373874682206	0.0
25	0.0	0.0	0.0	2.2391125531858687	0.0
26	0.0	0.0	0.0	2.3257402753322323	0.0
27	0.0	0.0	0.0	2.409908535335639	0.0
28	0.0	0.0	0.0	2.505463194149031	0.0
29	0.0	0.0	0.0	2.605572412486416	0.0
30	0.0	0.0	0.0	2.7277256989199317	0.0
31	0.0	0.0	0.0	2.8334825710670697	0.0
32	0.0	0.0	0.0	2.9405147198809254	0.0
33	0.0	0.0	0.0	3.053923252028373	0.0
34	0.0	0.0	0.0	3.173070529176053	0.0
35	0.0	0.0	0.0	3.306792396800513	0.0
36	0.0	0.0	0.0	3.4206563849003597	0.0
37	0.0	0.0	0.0	3.5375263822860425	0.0
38	0.0	0.0	0.0	3.6727968001486606	0.0
39	0.0	0.0	0.0	3.855616819441775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	70	0.0	45.0	1
TATATCG	20	7.0331484E-4	45.0	27
CAAATCG	35	1.2118653E-7	45.0	1
TATACGC	20	7.0331484E-4	45.0	12
ATTACCG	30	2.1654123E-6	44.999996	1
ACAATCG	30	2.1654123E-6	44.999996	14
TAATCGC	30	2.1654123E-6	44.999996	16
CGGTCTA	200	0.0	43.875	31
AGCGATA	120	0.0	43.124996	8
CGTTTTT	3840	0.0	42.656246	1
GCGTAAG	75	0.0	42.000004	1
CGACGGT	215	0.0	41.860462	28
TATACGA	60	3.6379788E-12	41.249996	2
TCTAGCG	55	6.184564E-11	40.909092	1
AGGGGAA	9705	0.0	40.479137	8
GAGGGGA	9555	0.0	40.149136	7
TACGTAG	45	1.9281288E-8	40.000004	1
GCGATAA	75	0.0	39.000004	9
GAGACCG	180	0.0	38.750004	7
GAGCGAT	690	0.0	38.47826	7
>>END_MODULE