FastQCFastQC Report
Thu 26 May 2016
SRR1546934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546934_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1170257
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121671.0396861544088178No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69170.5910667485859944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC406.8157533E-945.00000412
TATAGCG406.8157533E-945.0000041
GCGAATA253.890849E-545.015
CGTTTTT96550.044.184361
ATTGCGA850.042.352942
TCACGAC1500.042.025
CGACGGT1500.042.028
AGCGATA1500.042.08
GTTTTTA67600.041.8380172
ATACGAG2450.041.326533
ACGACGG1550.040.6451627
CGCATCG501.0822987E-940.521
GCGATAT950.040.263169
GAGCGAT7500.039.97
CGTAAGA1200.039.3750042
TAGCGGA750.039.02
TACACGC2550.038.8235335
ACACGCG2500.038.69999736
TAAGCGA700.038.571432
TCGTAGA1000.038.252
Produced by FastQC (version 0.11.5)