##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546934_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1170257
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75880853521919	31.0	31.0	34.0	30.0	34.0
2	32.11218048685033	33.0	31.0	34.0	30.0	34.0
3	32.057847976982835	33.0	31.0	34.0	30.0	34.0
4	35.8444307532448	37.0	35.0	37.0	35.0	37.0
5	35.86188332990104	37.0	35.0	37.0	35.0	37.0
6	35.91401034131819	37.0	35.0	37.0	35.0	37.0
7	36.147034369373564	37.0	35.0	37.0	35.0	37.0
8	13.294243913943689	0.0	0.0	37.0	0.0	37.0
9	24.98263800173808	18.0	17.0	39.0	17.0	39.0
10	32.73716029897706	32.0	32.0	38.0	27.0	39.0
11	35.59585116773495	37.0	35.0	37.0	32.0	39.0
12	36.709397166605285	38.0	35.0	39.0	33.0	39.0
13	36.98455211120292	39.0	37.0	39.0	33.0	39.0
14	38.17608867112096	40.0	37.0	41.0	33.0	41.0
15	38.442917239546524	40.0	38.0	41.0	34.0	41.0
16	38.514846738793274	40.0	38.0	41.0	34.0	41.0
17	38.49385305962707	40.0	38.0	41.0	34.0	41.0
18	38.41621199445934	40.0	37.0	41.0	34.0	41.0
19	38.35775304057143	40.0	37.0	41.0	34.0	41.0
20	38.29721847423259	40.0	37.0	41.0	34.0	41.0
21	38.246674021176545	40.0	37.0	41.0	34.0	41.0
22	38.204831075567164	40.0	37.0	41.0	34.0	41.0
23	38.04166947943913	40.0	36.0	41.0	34.0	41.0
24	37.83406294514795	40.0	35.0	41.0	34.0	41.0
25	37.727582061034454	40.0	35.0	41.0	33.0	41.0
26	37.65987471128137	40.0	35.0	41.0	33.0	41.0
27	37.61812490760577	40.0	35.0	41.0	33.0	41.0
28	37.531341406203936	40.0	35.0	41.0	33.0	41.0
29	37.50146848085506	40.0	35.0	41.0	33.0	41.0
30	37.362149510748495	40.0	35.0	41.0	33.0	41.0
31	37.245525555497636	40.0	35.0	41.0	33.0	41.0
32	37.00660453216687	40.0	35.0	41.0	32.0	41.0
33	36.71783719302683	40.0	35.0	41.0	31.0	41.0
34	36.39826465468696	40.0	35.0	41.0	30.0	41.0
35	36.150904459447794	40.0	35.0	41.0	29.0	41.0
36	35.99779877411543	40.0	35.0	41.0	27.0	41.0
37	35.93231828564153	40.0	35.0	41.0	27.0	41.0
38	35.84242948343825	39.0	35.0	41.0	27.0	41.0
39	35.754369339384425	39.0	35.0	41.0	26.0	41.0
40	35.64465925006217	39.0	35.0	41.0	25.0	41.0
41	35.58420073539402	39.0	35.0	41.0	25.0	41.0
42	35.51514069131823	39.0	35.0	41.0	24.0	41.0
43	35.41656832644453	39.0	35.0	41.0	24.0	41.0
44	35.23689839069538	39.0	35.0	40.0	23.0	41.0
45	35.159408574355886	38.0	35.0	40.0	23.0	41.0
46	35.12563052389347	38.0	35.0	40.0	23.0	41.0
47	35.096013097977625	38.0	35.0	40.0	23.0	41.0
48	35.023815281600534	38.0	35.0	40.0	23.0	41.0
49	34.93486046227453	38.0	34.0	40.0	24.0	41.0
50	34.83518577543224	38.0	34.0	40.0	23.0	41.0
51	34.47345326710287	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	6.0
14	11.0
15	21.0
16	48.0
17	151.0
18	337.0
19	671.0
20	1367.0
21	2430.0
22	3894.0
23	6430.0
24	10665.0
25	16533.0
26	21484.0
27	22345.0
28	21150.0
29	21051.0
30	23120.0
31	28534.0
32	36823.0
33	51073.0
34	77378.0
35	115469.0
36	119671.0
37	207319.0
38	266134.0
39	116115.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.22801230840747	22.371410724310984	27.913184881611475	18.48739208567007
2	35.545781823992506	23.44185935226194	28.639862867729054	12.372495956016499
3	28.375561949212862	23.647797022363466	35.70472127062688	12.271919757796791
4	25.216255916435447	25.24599297419285	35.09391526818468	14.443835841187022
5	22.252377041965996	30.041520794150344	33.69191553650181	14.01418662738185
6	20.294516503639798	38.52376016550211	31.240830005716692	9.940893325141401
7	82.70439741014152	3.8415493348896863	11.763398979882195	1.6906542750865834
8	31.334741001335605	63.450934281956876	4.325289231339783	0.8890354853677441
9	79.41939249241832	4.851242077594922	12.822568034201035	2.906797395785712
10	42.76744339063983	26.23936451565767	19.422827635297203	11.57036445840529
11	34.92719975184938	22.578032004935668	27.01218621208846	15.482582031126496
12	31.10598783002366	21.203205791548353	31.088299407736937	16.602506970691053
13	22.95333418214973	27.62683752372342	32.255649827345614	17.16417846678123
14	18.16353160032369	30.763242603975023	33.3570318314695	17.71619396423179
15	17.919140838294496	24.38208017555118	41.122761923235664	16.57601706291866
16	21.163385478574366	22.12103837020415	39.23813316220283	17.477442989018652
17	21.190302642923733	21.869384246366398	31.840954593734537	25.09935851697533
18	23.28155268458125	23.889795147561603	34.23871850371329	18.58993366414386
19	25.598906906773472	25.540543658358807	30.140131612115972	18.720417822751752
20	26.6994343977434	24.826768820865844	30.009134745615707	18.46466203577505
21	24.27646234972318	25.646246935502205	32.25317173919917	17.824118975575452
22	22.93308222040116	22.400293268914435	32.66427801756366	22.002346493120744
23	20.998293537231564	26.66892827814745	31.830016825363998	20.502761359256983
24	21.61115037124324	24.411389976731606	35.49271655713232	18.48474309489283
25	21.31318163446149	24.670563816324105	34.37680782939132	19.63944671982308
26	19.312680889753274	28.472121935609017	31.08342868275943	21.13176849187828
27	19.35327026456582	26.970400518860387	33.90947458549703	19.766854631076765
28	18.20907715142913	26.292686136464045	36.96025744772302	18.537979264383804
29	19.38463089731572	25.809117142644734	35.7538557769789	19.052396183060644
30	20.85311175237576	25.510208441393644	34.95531323461428	18.68136657161632
31	25.943702964391584	24.22399524207076	31.00566798575014	18.82663380778752
32	25.96173319193989	25.2435148860464	30.778367486799908	18.0163844352138
33	25.055521992177788	25.057060115854895	30.080999301862754	19.806418590104567
34	20.80047374209255	26.57023200886643	31.765073825663936	20.864220423377088
35	20.575651331288768	26.793174490731523	31.84804705291231	20.7831271250674
36	25.363317630229943	26.317979725820912	29.321764364579746	18.996938279369406
37	21.803501282196986	29.232638642622945	28.839562591806754	20.12429748337331
38	21.87861298842904	30.897913877037265	26.40086750175389	20.82260563277981
39	21.752999554798645	27.97599159842667	28.63516304538234	21.635845801392346
40	22.595805878537792	26.063334805944333	28.956630893897668	22.384228421620207
41	20.399877975521616	25.667866118297095	29.49805042823927	24.434205477942026
42	22.144879287199306	27.260336831995026	27.50353127560869	23.091252605196978
43	21.70856487079334	26.876831328503055	28.083232999247176	23.331370801456433
44	20.869774758877753	28.064433709860314	30.022208796871112	21.043582734390824
45	20.898742754796594	29.829943337232763	27.726815562735364	21.544498345235276
46	22.42216880565551	28.51416398278327	28.870325065348894	20.193342146212327
47	21.820591545275953	27.28084514768978	29.512833505802572	21.385729801231694
48	22.106084390010057	26.340966129662117	30.486978501303557	21.065970979024264
49	21.419055814235676	25.36434304601468	31.35166036178378	21.864940777965867
50	20.463026497598392	28.086138343970596	30.22575383014158	21.225081328289427
51	19.75241335877504	28.98517163323954	29.298008898899987	21.96440610908544
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	767.0
1	1461.5
2	2156.0
3	16772.5
4	31389.0
5	21144.5
6	10900.0
7	10771.5
8	10643.0
9	10709.0
10	10775.0
11	10651.0
12	10527.0
13	9928.0
14	9329.0
15	8760.0
16	8191.0
17	7772.0
18	7353.0
19	7125.5
20	6898.0
21	6809.0
22	6720.0
23	7236.0
24	7752.0
25	8994.0
26	11296.0
27	12356.0
28	14369.5
29	16383.0
30	18716.5
31	21050.0
32	23581.5
33	26113.0
34	28862.0
35	31611.0
36	36405.0
37	41199.0
38	49065.0
39	56931.0
40	64902.0
41	72873.0
42	77998.0
43	83123.0
44	85132.0
45	87141.0
46	88808.5
47	90476.0
48	88475.5
49	86475.0
50	81664.0
51	76853.0
52	71527.0
53	66201.0
54	61930.5
55	57660.0
56	54891.0
57	52122.0
58	49779.0
59	47436.0
60	42157.0
61	36878.0
62	31779.0
63	26680.0
64	22127.0
65	17574.0
66	14548.0
67	11522.0
68	9578.0
69	7634.0
70	6192.5
71	4751.0
72	3801.5
73	2852.0
74	2167.5
75	1090.5
76	698.0
77	501.0
78	304.0
79	213.0
80	122.0
81	100.5
82	79.0
83	53.5
84	28.0
85	16.5
86	5.0
87	3.5
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1170257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.54116173996908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.51785873757957	21.84238491000679
2	6.9662128391783416	4.255124660727899
3	3.464352392164844	3.1741604020006595
4	2.361039657885532	2.884355762638532
5	1.9385011714641336	2.960203890540282
6	1.5677145030123223	2.8727893319156745
7	1.336038456873379	2.8562916621532
8	1.1373087104540693	2.7787783419402716
9	0.9922016797357439	2.7272692781530523
>10	8.575986966851957	48.325303213472615
>50	0.11697874027140195	2.2019233316055162
>100	0.024123135109866935	1.278858447713471
>500	0.0011220062792841293	0.2076602940081977
>1k	0.0	0.0
>5k	2.805015698210323E-4	0.5925685864912144
>10k+	2.805015698210323E-4	1.0423278866327317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12167	1.0396861544088178	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6917	0.5910667485859944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.563539461844706E-4	0.0	0.0	0.006152494708427294	0.0
2	2.563539461844706E-4	0.0	0.0	0.020508315694757646	0.0
3	2.563539461844706E-4	0.0	0.0	0.036060455096615526	0.0
4	2.563539461844706E-4	0.0	0.0	0.053919780014133646	0.0
5	2.563539461844706E-4	0.0	0.0	0.10373789688931577	0.0
6	2.563539461844706E-4	0.0	0.0	0.18927466359953413	0.0
7	2.563539461844706E-4	0.0	0.0	0.25361950409183626	0.0
8	2.563539461844706E-4	0.0	0.0	0.35693014440417786	0.0
9	2.563539461844706E-4	0.0	0.0	0.5232184041625044	0.0
10	2.563539461844706E-4	0.0	0.0	0.7404356478961459	0.0
11	2.563539461844706E-4	0.0	0.0	0.9372300272504245	0.0
12	2.563539461844706E-4	0.0	0.0	1.1059963751552009	0.0
13	2.563539461844706E-4	0.0	0.0	1.184440682687649	0.0
14	2.563539461844706E-4	0.0	0.0	1.221184748307423	0.0
15	2.563539461844706E-4	0.0	0.0	1.2576724599810127	0.0
16	2.563539461844706E-4	0.0	0.0	1.3230427162580527	0.0
17	2.563539461844706E-4	0.0	0.0	1.40182882905208	0.0
18	2.563539461844706E-4	0.0	0.0	1.5210334140278587	0.0
19	2.563539461844706E-4	0.0	0.0	1.5851219005739765	0.0
20	2.563539461844706E-4	0.0	0.0	1.6581827752365506	0.0
21	2.563539461844706E-4	0.0	0.0	1.7462830814086137	0.0
22	2.563539461844706E-4	0.0	0.0	1.8395104665043662	0.0
23	2.563539461844706E-4	0.0	0.0	1.950938981779216	0.0
24	2.563539461844706E-4	0.0	0.0	2.0305796077271916	0.0
25	2.563539461844706E-4	0.0	0.0	2.1038113850205553	0.0
26	2.563539461844706E-4	0.0	0.0	2.177897675467867	0.0
27	2.563539461844706E-4	0.0	0.0	2.2599309382468977	0.0
28	2.563539461844706E-4	0.0	0.0	2.34606586416488	0.0
29	2.563539461844706E-4	0.0	0.0	2.432884400606021	0.0
30	2.563539461844706E-4	0.0	0.0	2.549525446119955	0.0
31	2.563539461844706E-4	0.0	0.0	2.6528360864322966	0.0
32	2.563539461844706E-4	0.0	0.0	2.7500796833516055	0.0
33	2.563539461844706E-4	0.0	0.0	2.8568083762797403	0.0
34	2.563539461844706E-4	0.0	0.0	2.9570427692378685	0.0
35	2.563539461844706E-4	0.0	0.0	3.084023423914576	0.0
36	2.563539461844706E-4	0.0	0.0	3.1946828773508726	0.0
37	2.563539461844706E-4	0.0	0.0	3.3132893031188875	0.0
38	2.563539461844706E-4	0.0	0.0	3.4556511945666637	0.0
39	2.563539461844706E-4	0.0	0.0	3.659708935729502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCC	40	6.8157533E-9	45.000004	12
TATAGCG	40	6.8157533E-9	45.000004	1
GCGAATA	25	3.890849E-5	45.0	15
CGTTTTT	9655	0.0	44.18436	1
ATTGCGA	85	0.0	42.35294	2
TCACGAC	150	0.0	42.0	25
CGACGGT	150	0.0	42.0	28
AGCGATA	150	0.0	42.0	8
GTTTTTA	6760	0.0	41.838017	2
ATACGAG	245	0.0	41.32653	3
ACGACGG	155	0.0	40.64516	27
CGCATCG	50	1.0822987E-9	40.5	21
GCGATAT	95	0.0	40.26316	9
GAGCGAT	750	0.0	39.9	7
CGTAAGA	120	0.0	39.375004	2
TAGCGGA	75	0.0	39.0	2
TACACGC	255	0.0	38.82353	35
ACACGCG	250	0.0	38.699997	36
TAAGCGA	70	0.0	38.57143	2
TCGTAGA	100	0.0	38.25	2
>>END_MODULE