FastQCFastQC Report
Thu 26 May 2016
SRR1546933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546933_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences692166
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18860.2724779893840495No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17040.24618371893447527No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.1424513772707703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC351.2109922E-745.00000425
ACGTTAG351.2109922E-745.0000041
TGGTTAG351.2109922E-745.0000041
TATAGCG700.045.0000041
ACGTAAG351.2109922E-745.0000041
CGACTTA351.2109922E-745.00000428
GTCATAG207.0313405E-445.09
CGTGGTA253.889193E-545.038
CGGAAAT253.889193E-545.05
GGTACGC207.0313405E-445.09
TGCCCCG207.0313405E-445.040
CGTATCT207.0313405E-445.034
TTAATCT253.889193E-545.015
ACGTTCA207.0313405E-445.017
GTTTACG551.8189894E-1245.01
CGATTCC207.0313405E-445.022
CACGACC253.889193E-545.037
GACCCTC453.8380676E-1045.09
TAGCCCG253.889193E-545.01
GTTACGA253.889193E-545.02