##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546933_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	692166
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.796125495907052	31.0	31.0	34.0	30.0	34.0
2	32.13917181716525	33.0	31.0	34.0	30.0	34.0
3	32.155201208958545	33.0	31.0	34.0	30.0	34.0
4	35.86501792922507	37.0	35.0	37.0	35.0	37.0
5	35.838323465758215	37.0	35.0	37.0	35.0	37.0
6	35.895955594467225	37.0	35.0	37.0	35.0	37.0
7	36.14951760126906	37.0	35.0	37.0	35.0	37.0
8	13.233000465206324	0.0	0.0	37.0	0.0	37.0
9	24.9952135759341	18.0	17.0	39.0	17.0	39.0
10	32.86912821490798	32.0	32.0	38.0	27.0	39.0
11	35.722290606588594	37.0	35.0	37.0	32.0	39.0
12	36.451329594345864	37.0	35.0	39.0	33.0	39.0
13	36.357040074201855	38.0	35.0	39.0	33.0	39.0
14	37.46593302762632	39.0	35.0	41.0	33.0	41.0
15	37.93921544831731	40.0	36.0	41.0	33.0	41.0
16	38.14118000595233	40.0	36.0	41.0	34.0	41.0
17	38.16540829800944	40.0	36.0	41.0	34.0	41.0
18	38.117308564708466	40.0	36.0	41.0	34.0	41.0
19	38.07056399765374	40.0	36.0	41.0	34.0	41.0
20	38.0334746289185	40.0	36.0	41.0	34.0	41.0
21	37.91840107719825	40.0	35.0	41.0	34.0	41.0
22	37.86473042593828	40.0	35.0	41.0	33.0	41.0
23	37.803463331050644	40.0	35.0	41.0	33.0	41.0
24	37.72954609154451	40.0	35.0	41.0	33.0	41.0
25	37.628509634971955	40.0	35.0	41.0	33.0	41.0
26	37.52738360451106	40.0	35.0	41.0	33.0	41.0
27	37.42047138981111	39.0	35.0	41.0	33.0	41.0
28	37.45265731052955	39.0	35.0	41.0	33.0	41.0
29	37.53066894357712	40.0	35.0	41.0	33.0	41.0
30	37.47169031706267	40.0	35.0	41.0	33.0	41.0
31	37.34196710037766	40.0	35.0	41.0	33.0	41.0
32	37.13754359503356	39.0	35.0	41.0	33.0	41.0
33	36.997185646217815	39.0	35.0	41.0	32.0	41.0
34	36.90082292398066	39.0	35.0	41.0	32.0	41.0
35	36.80610720549695	39.0	35.0	41.0	32.0	41.0
36	36.67501004094394	39.0	35.0	41.0	31.0	41.0
37	36.56336341282294	39.0	35.0	41.0	31.0	41.0
38	36.51153769471485	39.0	35.0	41.0	31.0	41.0
39	36.49123042738303	39.0	35.0	41.0	31.0	41.0
40	36.34170849189356	39.0	35.0	41.0	30.0	41.0
41	36.36637598495159	39.0	35.0	41.0	31.0	41.0
42	36.31501113894644	39.0	35.0	41.0	31.0	41.0
43	36.217897151839296	39.0	35.0	41.0	30.0	41.0
44	36.02798895062745	38.0	35.0	40.0	30.0	41.0
45	35.92203748811701	38.0	35.0	40.0	30.0	41.0
46	35.92085569068691	38.0	35.0	40.0	30.0	41.0
47	35.891723950613006	38.0	35.0	40.0	30.0	41.0
48	35.871642062742175	38.0	35.0	40.0	30.0	41.0
49	35.83027626320854	38.0	35.0	40.0	29.0	41.0
50	35.670152824611435	38.0	35.0	40.0	29.0	41.0
51	35.20494505653268	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	5.0
15	7.0
16	22.0
17	77.0
18	165.0
19	290.0
20	651.0
21	1158.0
22	1773.0
23	2803.0
24	4205.0
25	6036.0
26	8107.0
27	8926.0
28	9261.0
29	10623.0
30	12937.0
31	17173.0
32	23097.0
33	32802.0
34	54961.0
35	93211.0
36	67259.0
37	116549.0
38	169391.0
39	50658.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.501113894643773	22.982059217008636	31.51772840619158	13.999098482156015
2	29.962176703276384	25.49489573310449	32.100825524512906	12.442102039106226
3	29.581487677811392	24.2873819286125	32.83128613656262	13.29984425701349
4	25.295088172490416	28.067400016758988	32.74286804032558	13.894643770425013
5	22.62968709818165	31.89162715302399	31.372098600624703	14.106587148169659
6	21.591063415423466	40.071890269097295	27.337083878722733	10.999962436756501
7	88.6200709078458	4.310382191555205	5.795286101888853	1.274260798710136
8	33.741183473328654	63.57680094081477	1.9532886619683716	0.7287269238882003
9	88.05720015140876	3.7345087739068377	6.097670212058957	2.1106208626254395
10	61.5473744737534	19.789327993573796	10.39736132661818	8.265936206054617
11	56.116451833808654	16.547909027603204	16.295657400103444	11.039981738484698
12	48.432168005940774	20.085210773138236	19.724314687517154	11.758306533403836
13	20.803824516084294	47.72569008012529	20.221738715857178	11.248746687933242
14	12.928690516436808	49.389886241161804	25.850879702267953	11.830543540133435
15	11.466469026216254	22.294218438929388	54.10262856020087	12.13668397465348
16	13.63906924061569	16.001074886660135	53.001158681587945	17.35869719113623
17	14.672491858889341	15.981137472802768	27.39602927621409	41.950341392093804
18	20.608495649887455	21.224099421237103	36.21631227191165	21.95109265696379
19	30.126877078619867	23.144592482150237	25.46311145014346	21.26541898908643
20	34.30679923602142	21.58875183120812	23.206282885897313	20.898166046873147
21	22.7796511241523	28.71926676548689	27.340262307018836	21.160819803341973
22	22.603392827732076	22.6783748407174	25.30693504159407	29.411297289956455
23	18.04567112513472	30.955868967848755	24.409751418012444	26.58870848900408
24	19.390724190439865	23.418948633709256	40.70743145430431	16.482895721546566
25	15.47388921154752	23.004308215081355	39.49587237743547	22.025930195935654
26	14.241381402727091	38.158765382870584	26.982833597720777	20.61701961668155
27	15.929271301970916	36.84231817223036	29.745610157101044	17.48280036869768
28	12.99890488697798	27.760103790131268	43.724915699413145	15.516075623477604
29	12.939381593432788	22.953742310370632	43.400860487224165	20.706015608972415
30	16.87167529176527	31.228491431246262	33.713588936757944	18.186244340230523
31	32.68276685072656	25.116807239881762	23.59130613176608	18.609119777625597
32	32.76511703839831	26.454636604513947	25.415579499715385	15.364666857372363
33	29.817124793763345	27.770361445086873	23.955091697656343	18.45742206349344
34	19.037196279505206	29.099088946871127	26.48815457563648	25.37556019798719
35	18.514633772823284	25.6372026363618	32.5664652698919	23.281698320923017
36	33.37407500512883	21.64292958625532	27.8226610379591	17.16033437065675
37	20.492627491093177	32.216982631334105	29.342094237509496	17.948295640063222
38	20.14531196273726	34.077952398702045	22.8228488541766	22.953886784384093
39	19.62087129388037	31.714935434563387	27.681943348849842	20.9822499227064
40	24.407584307810552	26.405515439937822	24.099132289075165	25.087767963176468
41	17.415475478425694	22.473510689632256	27.17036086719082	32.94065296475123
42	23.181288881568875	23.432240242947504	23.750660968611577	29.63580990687205
43	23.340210296373993	25.68733511903214	24.161689536902998	26.81076504769087
44	19.121569103365378	31.15278704819364	28.40705842240156	21.31858542603942
45	16.33408748768359	40.93324433734104	20.96693567727973	21.765732497695637
46	23.31723892823398	33.526206141301365	24.684974413652217	18.47158051681244
47	21.89662595388967	27.555239639046125	25.55543034474389	24.99270406232031
48	23.67582342963971	23.25034746000237	31.21606666608877	21.85776244426915
49	21.49311003429813	21.159519537220838	34.17489446173317	23.17247596674786
50	19.426987167818126	33.78625936552792	26.280111996255233	20.50664147039872
51	17.88877234651803	36.55972700190417	23.098071849816375	22.453428801761426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	897.0
1	1012.0
2	1127.0
3	2978.5
4	4830.0
5	3634.5
6	2439.0
7	2732.5
8	3026.0
9	3154.0
10	3282.0
11	3347.0
12	3412.0
13	3189.5
14	2967.0
15	2760.0
16	2553.0
17	2464.5
18	2376.0
19	2403.5
20	2431.0
21	2350.0
22	2269.0
23	2385.5
24	2502.0
25	2987.0
26	4090.0
27	4708.0
28	5925.0
29	7142.0
30	8309.0
31	9476.0
32	10353.0
33	11230.0
34	12745.0
35	14260.0
36	17727.0
37	21194.0
38	32110.0
39	43026.0
40	56556.5
41	70087.0
42	74894.5
43	79702.0
44	75762.5
45	71823.0
46	68550.5
47	65278.0
48	61024.5
49	56771.0
50	52604.0
51	48437.0
52	42846.5
53	37256.0
54	32698.0
55	28140.0
56	25612.5
57	23085.0
58	21115.5
59	19146.0
60	16703.5
61	14261.0
62	12482.0
63	10703.0
64	9025.5
65	7348.0
66	6000.5
67	4653.0
68	3682.0
69	2711.0
70	2333.5
71	1956.0
72	1521.5
73	1087.0
74	829.0
75	436.0
76	301.0
77	224.0
78	147.0
79	94.0
80	41.0
81	30.0
82	19.0
83	17.5
84	16.0
85	10.5
86	5.0
87	2.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	692166.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.224399034401102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97316468769456	16.54933561306703
2	4.9211495790711295	2.0889688474836134
3	1.7889673031273208	1.1390926770321197
4	1.0538775743848028	0.8947167268859915
5	0.7689997793816753	0.8160779087481547
6	0.6251470602722087	0.7961022397440093
7	0.546976527541518	0.8126473658094586
8	0.4656847801751757	0.7907103678948232
9	0.44293202470936993	0.8460869433792189
>10	9.871057084638663	52.04410619175308
>50	1.4325331791897455	19.407990553725977
>100	0.10677265931849035	3.056154559241975
>500	0.0013688802476729532	0.2364967973403335
>1k	0.0013688802476729532	0.5215132078942243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1886	0.2724779893840495	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1704	0.24618371893447527	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	986	0.1424513772707703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.013291609238246316	0.0
2	0.0	0.0	0.0	0.04131956784933094	0.0
3	0.0	0.0	0.0	0.0625572478278332	0.0
4	0.0	0.0	0.0	0.10012049132722498	0.0
5	0.0	0.0	0.0	0.16513379738386458	0.0
6	0.0	0.0	0.0	0.2379487001673009	0.0
7	0.0	0.0	0.0	0.2831690663800302	0.0
8	0.0	0.0	0.0	0.37447664288624405	0.0
9	0.0	0.0	0.0	0.46997396578277467	0.0
10	0.0	0.0	0.0	0.6494106904990999	0.0
11	0.0	0.0	0.0	0.8665551327282761	0.0
12	0.0	0.0	0.0	1.0494592337676223	0.0
13	0.0	0.0	0.0	1.12198518852414	0.0
14	0.0	0.0	0.0	1.1469791928525817	0.0
15	0.0	0.0	0.0	1.1969672015094646	0.0
16	0.0	0.0	0.0	1.3012774392270063	0.0
17	0.0	0.0	0.0	1.4141116437386407	0.0
18	0.0	0.0	0.0	1.6133413082988763	0.0
19	0.0	0.0	0.0	1.6903459574726294	0.0
20	0.0	0.0	0.0	1.7722627231039954	0.0
21	0.0	0.0	0.0	1.8892866740059466	0.0
22	0.0	0.0	0.0	1.9908519054677636	0.0
23	0.0	0.0	0.0	2.124779315944441	0.0
24	0.0	0.0	0.0	2.217531632585247	0.0
25	0.0	0.0	0.0	2.280955724493835	0.0
26	0.0	0.0	0.0	2.3625835420982826	0.0
27	0.0	0.0	0.0	2.430197380397188	0.0
28	0.0	0.0	0.0	2.5037346532479203	0.0
29	0.0	0.0	0.0	2.5878185290811744	0.0
30	0.0	0.0	0.0	2.7087432783465237	0.0
31	0.0	0.0	0.0	2.825622755235016	0.0
32	0.0	0.0	0.0	2.9102845271221067	0.0
33	0.0	0.0	0.0	3.0024589477090755	0.0
34	0.0	0.0	0.0	3.1025794390363006	0.0
35	0.0	0.0	0.0	3.2544216271819186	0.0
36	0.0	0.0	0.0	3.3578650208187053	0.0
37	0.0	0.0	0.0	3.4624642065631654	0.0
38	0.0	0.0	0.0	3.5614289057827166	0.0
39	0.0	0.0	0.0	3.668195201729065	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	35	1.2109922E-7	45.000004	25
ACGTTAG	35	1.2109922E-7	45.000004	1
TGGTTAG	35	1.2109922E-7	45.000004	1
TATAGCG	70	0.0	45.000004	1
ACGTAAG	35	1.2109922E-7	45.000004	1
CGACTTA	35	1.2109922E-7	45.000004	28
GTCATAG	20	7.0313405E-4	45.0	9
CGTGGTA	25	3.889193E-5	45.0	38
CGGAAAT	25	3.889193E-5	45.0	5
GGTACGC	20	7.0313405E-4	45.0	9
TGCCCCG	20	7.0313405E-4	45.0	40
CGTATCT	20	7.0313405E-4	45.0	34
TTAATCT	25	3.889193E-5	45.0	15
ACGTTCA	20	7.0313405E-4	45.0	17
GTTTACG	55	1.8189894E-12	45.0	1
CGATTCC	20	7.0313405E-4	45.0	22
CACGACC	25	3.889193E-5	45.0	37
GACCCTC	45	3.8380676E-10	45.0	9
TAGCCCG	25	3.889193E-5	45.0	1
GTTACGA	25	3.889193E-5	45.0	2
>>END_MODULE