FastQCFastQC Report
Thu 26 May 2016
SRR1546932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546932_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1132163
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93210.8232913458574428No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.4576196183765059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA253.8907696E-545.026
CGTAAGA700.045.02
ACGTAAC253.8907696E-545.038
CGTTTTT70950.043.4778021
TACGTAG700.041.7857131
TCGTAAG603.6379788E-1241.2500041
ACGTAGA1150.041.0869562
TAGCGGA556.184564E-1140.9090922
AGGGGAA76450.040.2321788
GTTTTTA51850.040.1398242
CGCATCG451.9283107E-840.00000421
CGGTCTA1800.040.00000431
CTATACG403.4581535E-739.3751
TAGAGGC5850.039.230775
GAGCGAT6600.039.2045447
CGTTAGA1150.039.1304322
GAGGGGA77800.039.1291777
TCACGAC1850.038.9189225
GCGCGAC3500.038.5714269
CGCGAAC356.248207E-638.57142621
Produced by FastQC (version 0.11.5)