##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546932_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1132163
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.760463820138973	31.0	31.0	34.0	30.0	34.0
2	32.109692685593856	33.0	31.0	34.0	30.0	34.0
3	32.08698835768348	33.0	31.0	34.0	30.0	34.0
4	35.858190914205814	37.0	35.0	37.0	35.0	37.0
5	35.859388621603074	37.0	35.0	37.0	35.0	37.0
6	35.90642336836657	37.0	35.0	37.0	35.0	37.0
7	36.11862691149596	37.0	35.0	37.0	35.0	37.0
8	13.24052631997336	0.0	0.0	36.0	0.0	37.0
9	24.925961191100576	18.0	17.0	39.0	17.0	39.0
10	32.7178453985866	32.0	32.0	38.0	27.0	39.0
11	35.600376447561	37.0	35.0	37.0	32.0	39.0
12	36.66789499391872	37.0	35.0	39.0	33.0	39.0
13	36.8996151614211	39.0	37.0	39.0	33.0	39.0
14	38.07983744390163	40.0	37.0	41.0	33.0	41.0
15	38.366286479950325	40.0	37.0	41.0	34.0	41.0
16	38.46294482331608	40.0	38.0	41.0	34.0	41.0
17	38.42727769764601	40.0	37.0	41.0	34.0	41.0
18	38.36143205527826	40.0	37.0	41.0	34.0	41.0
19	38.324971757600274	40.0	37.0	41.0	34.0	41.0
20	38.28163170850841	40.0	37.0	41.0	34.0	41.0
21	38.21069934276248	40.0	37.0	41.0	34.0	41.0
22	38.162873190521154	40.0	37.0	41.0	34.0	41.0
23	38.04138979987864	40.0	36.0	41.0	34.0	41.0
24	37.884428302285095	40.0	35.0	41.0	34.0	41.0
25	37.78512987970814	40.0	35.0	41.0	33.0	41.0
26	37.712253447604276	40.0	35.0	41.0	33.0	41.0
27	37.66478766750017	40.0	35.0	41.0	33.0	41.0
28	37.586656691660124	40.0	35.0	41.0	33.0	41.0
29	37.56326165048672	40.0	35.0	41.0	33.0	41.0
30	37.446093892840516	40.0	35.0	41.0	33.0	41.0
31	37.34385066461278	40.0	35.0	41.0	33.0	41.0
32	37.14874889923094	40.0	35.0	41.0	32.0	41.0
33	36.93656478793248	40.0	35.0	41.0	31.0	41.0
34	36.6770129389496	40.0	35.0	41.0	31.0	41.0
35	36.49290782334346	40.0	35.0	41.0	30.0	41.0
36	36.351313370954536	40.0	35.0	41.0	30.0	41.0
37	36.28748422267818	39.0	35.0	41.0	30.0	41.0
38	36.18106138427064	39.0	35.0	41.0	29.0	41.0
39	36.09692332287842	39.0	35.0	41.0	29.0	41.0
40	35.976722433077214	39.0	35.0	41.0	29.0	41.0
41	35.90876667052359	39.0	35.0	41.0	28.0	41.0
42	35.85960060521321	39.0	35.0	41.0	28.0	41.0
43	35.76136121742187	39.0	35.0	41.0	27.0	41.0
44	35.567026126096685	39.0	35.0	40.0	27.0	41.0
45	35.481503988383295	38.0	35.0	40.0	26.0	41.0
46	35.457904029720105	38.0	35.0	40.0	27.0	41.0
47	35.40976961797904	38.0	35.0	40.0	26.0	41.0
48	35.32800400649023	38.0	35.0	40.0	26.0	41.0
49	35.22401014694881	38.0	34.0	40.0	26.0	41.0
50	35.122916929806046	38.0	34.0	40.0	26.0	41.0
51	34.763979215007026	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	1.0
13	5.0
14	10.0
15	31.0
16	54.0
17	134.0
18	322.0
19	660.0
20	1250.0
21	2219.0
22	3574.0
23	5462.0
24	8562.0
25	13012.0
26	16820.0
27	18550.0
28	18344.0
29	19263.0
30	22066.0
31	27667.0
32	36599.0
33	51184.0
34	77373.0
35	117283.0
36	122580.0
37	204593.0
38	256555.0
39	107973.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.641478303035875	23.046063155217052	25.64189078781059	16.670567753936492
2	35.80509166966241	23.713281568113427	27.884412403514336	12.597214358709833
3	29.50281894038226	24.68646299163636	32.85754789725508	12.953170170726299
4	26.838096634495212	25.40499910348598	32.906480780594315	14.850423481424494
5	23.316077278625073	30.37009688534248	31.573280525860675	14.740545310171768
6	21.972631149401632	38.45532842885698	29.33031727763582	10.241723144105574
7	82.94865668636054	4.632901799475871	10.23403873823822	2.184402775925375
8	31.18013925556656	63.867570305689206	3.8051941284073054	1.1470963103369392
9	78.68451804201338	5.671268183114975	11.79300153776444	3.851212237107201
10	44.68031546694248	25.306161745261065	18.008007680872808	12.00551510692365
11	37.60739398832147	22.185939657098846	24.71304926940732	15.493617085172366
12	33.35332456545569	20.814759005549554	29.602362910640963	16.229553518353807
13	23.66178721615174	28.732435170554066	29.776366123959182	17.82941148933502
14	18.423230577222537	31.714161300095483	32.38129138648764	17.481316736194348
15	18.412631396715845	23.217681552921267	40.94966890809892	17.420018142263967
16	21.760294233250864	21.24164100045665	38.77153731397334	18.226527452319143
17	21.804634138370535	21.428009924366016	30.145482585104794	26.621873352158655
18	23.89523416681167	23.42701536792847	33.05990391842871	19.617846546831156
19	27.25729422353495	24.317081550978084	28.581661827846343	19.84396239764062
20	28.920482298043655	24.534629730877974	27.608833710340296	18.936054260738075
21	25.453137048287218	25.253960781265594	30.31604106475834	18.976861105688844
22	24.11967181404091	22.150962361426757	30.5149523522673	23.214413472265036
23	22.014586238907295	25.85263782688535	29.979163777653923	22.15361215655343
24	22.435638684535707	24.22213055893895	34.17007975000067	19.17215100652468
25	21.7608241922762	24.46917979124914	32.758710539030155	21.011285477444503
26	20.17324360538191	28.456679824371577	29.407426315822015	21.962650254424496
27	19.848378722851745	27.568380171406414	31.585822889460264	20.997418216281577
28	18.94276707505898	25.98565754224436	35.66995211820206	19.4016232644946
29	20.123957416025785	24.742815301330285	34.853903545690855	20.27932373695307
30	21.479857582344593	25.73489859675683	33.090288235881225	19.69495558501735
31	27.539939037046786	23.981970793958112	28.27711204128734	20.200978127707756
32	28.134906369489197	25.03632427486148	28.421525875691046	18.407243479958275
33	26.95274443697595	24.74520011694429	27.89889795020682	20.403157495872946
34	21.912392473521923	26.123093582814487	30.52369667618532	21.44081726747827
35	22.451537455295746	26.40335358071232	30.18929253119913	20.955816432792805
36	27.410452381856675	25.636856177069912	28.032800930608047	18.919890510465365
37	23.133594720901495	29.136705580380212	27.586398778267796	20.1433009204505
38	22.49631899293653	30.629600154748037	25.972938525636323	20.90114232667911
39	23.13915929066751	27.523598633765634	27.281230706179233	22.056011369387623
40	23.88375172126275	25.6336764229179	27.738673671547293	22.74389818427205
41	20.63139318278375	25.203526347354575	28.121834046864276	26.043246422997395
42	22.174898844071038	26.472248254005827	27.17903694079386	24.17381596112927
43	22.33282663362078	26.339051885638376	27.35286350110364	23.975257979637206
44	21.855510204802666	26.972352920913334	29.21902588231553	21.953110991968472
45	21.15481604680598	29.671080930926024	26.338786906125712	22.835316116142288
46	22.702119747774834	28.473550186678064	28.37718597057138	20.447144094975723
47	22.594979698153004	26.42437528871726	28.650291521627185	22.33035349150255
48	22.456042107011093	25.65990939467197	30.31524612622034	21.5688023720966
49	22.283805423777316	24.688759480746146	30.893254769852046	22.13418032562449
50	20.922517340700942	28.233655401209894	29.10066836665745	21.74315889143171
51	20.954049902708356	29.599448136001616	27.832123113014646	21.614378848275383
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	906.0
1	1753.5
2	2601.0
3	13022.0
4	23443.0
5	15757.5
6	8072.0
7	7924.5
8	7777.0
9	7871.0
10	7965.0
11	7828.5
12	7692.0
13	7280.0
14	6868.0
15	6439.5
16	6011.0
17	5778.5
18	5546.0
19	5258.5
20	4971.0
21	5062.0
22	5153.0
23	5846.0
24	6539.0
25	7769.0
26	10302.0
27	11605.0
28	13849.0
29	16093.0
30	16952.5
31	17812.0
32	21067.5
33	24323.0
34	27308.5
35	30294.0
36	33466.5
37	36639.0
38	43571.5
39	50504.0
40	59935.5
41	69367.0
42	74810.5
43	80254.0
44	80881.0
45	81508.0
46	82583.0
47	83658.0
48	81652.5
49	79647.0
50	77348.5
51	75050.0
52	70903.0
53	66756.0
54	63451.0
55	60146.0
56	57765.0
57	55384.0
58	52797.0
59	50210.0
60	46451.5
61	42693.0
62	37952.0
63	33211.0
64	28107.5
65	23004.0
66	19120.5
67	15237.0
68	12826.5
69	10416.0
70	8727.0
71	7038.0
72	5627.5
73	4217.0
74	3204.5
75	1650.5
76	1109.0
77	851.0
78	593.0
79	457.0
80	321.0
81	243.5
82	166.0
83	134.5
84	103.0
85	70.5
86	38.0
87	28.5
88	19.0
89	11.5
90	4.0
91	5.0
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1132163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.29657699762485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03458204682693	20.383320973797623
2	5.936197236570758	3.359481243554246
3	3.117822659752754	2.6467112686989993
4	2.1680034818841043	2.4538830982500928
5	1.7785977200638334	2.5164113666793133
6	1.4507852346956502	2.463135366034962
7	1.2877135849591435	2.5506520625378752
8	1.1646127968282343	2.6363644542295486
9	1.0408988919263518	2.650848807792276
>10	9.847555820650646	52.92112207294155
>50	0.14415079890808777	2.5238255690529456
>100	0.027203615519019914	1.3571827327444719
>500	0.0012507409430039774	0.25393535707917164
>1k	0.0	0.0
>5k	6.253704715019887E-4	1.28312562660702
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9321	0.8232913458574428	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5181	0.4576196183765059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009715915464469338	0.0
2	0.0	0.0	0.0	0.02720456330051415	0.0
3	0.0	0.0	0.0	0.045046517153448755	0.0
4	0.0	0.0	0.0	0.07057287687373638	0.0
5	0.0	0.0	0.0	0.14653367050504212	0.0
6	0.0	0.0	0.0	0.22231781112790297	0.0
7	0.0	0.0	0.0	0.2739004895938129	0.0
8	0.0	0.0	0.0	0.3582523011262513	0.0
9	0.0	0.0	0.0	0.49816148381460973	0.0
10	0.0	0.0	0.0	0.7357597801729963	0.0
11	0.0	0.0	0.0	0.9311380075130524	0.0
12	0.0	0.0	0.0	1.1033746907468271	0.0
13	0.0	0.0	0.0	1.1780989133190185	0.0
14	0.0	0.0	0.0	1.2168742486726734	0.0
15	0.0	0.0	0.0	1.2636872959105712	0.0
16	0.0	0.0	0.0	1.3407963340967688	0.0
17	0.0	0.0	0.0	1.4246181866038725	0.0
18	0.0	0.0	0.0	1.5397959481099452	0.0
19	0.0	0.0	0.0	1.6121353550681305	0.0
20	0.0	0.0	0.0	1.6843864355220934	0.0
21	0.0	0.0	0.0	1.774037837307879	0.0
22	0.0	0.0	0.0	1.8721685834990192	0.0
23	0.0	0.0	0.0	1.9698576971690471	0.0
24	0.0	0.0	0.0	2.0557110592732672	0.0
25	0.0	0.0	0.0	2.129905322820124	0.0
26	0.0	0.0	0.0	2.202774688803644	0.0
27	0.0	0.0	0.0	2.285713276268523	0.0
28	0.0	0.0	0.0	2.3668853336489533	0.0
29	0.0	0.0	0.0	2.4553001643756245	0.0
30	0.0	0.0	0.0	2.564913356115683	0.0
31	0.0	0.0	0.0	2.661012592709707	0.0
32	0.0	0.0	0.0	2.7485441583941537	0.0
33	0.0	0.0	0.0	2.844113435962843	0.0
34	0.0	0.0	0.0	2.948338710945332	0.0
35	0.0	0.0	0.0	3.072437449377872	0.0
36	0.0	0.0	0.0	3.1797541520081474	0.0
37	0.0	0.0	0.0	3.2869825281341996	0.0
38	0.0	0.0	0.0	3.415939224298975	0.0
39	0.0	0.0	0.0	3.6039863517885675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	25	3.8907696E-5	45.0	26
CGTAAGA	70	0.0	45.0	2
ACGTAAC	25	3.8907696E-5	45.0	38
CGTTTTT	7095	0.0	43.477802	1
TACGTAG	70	0.0	41.785713	1
TCGTAAG	60	3.6379788E-12	41.250004	1
ACGTAGA	115	0.0	41.086956	2
TAGCGGA	55	6.184564E-11	40.909092	2
AGGGGAA	7645	0.0	40.232178	8
GTTTTTA	5185	0.0	40.139824	2
CGCATCG	45	1.9283107E-8	40.000004	21
CGGTCTA	180	0.0	40.000004	31
CTATACG	40	3.4581535E-7	39.375	1
TAGAGGC	585	0.0	39.23077	5
GAGCGAT	660	0.0	39.204544	7
CGTTAGA	115	0.0	39.130432	2
GAGGGGA	7780	0.0	39.129177	7
TCACGAC	185	0.0	38.91892	25
GCGCGAC	350	0.0	38.571426	9
CGCGAAC	35	6.248207E-6	38.571426	21
>>END_MODULE