FastQCFastQC Report
Thu 26 May 2016
SRR1546931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546931_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1287971
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96440.7487746230311086No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54810.4255530598126821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG551.8189894E-1245.01
CGCGATT207.0336036E-445.012
CGTTTTT75250.043.1461751
TCACGAC2000.040.525
GTTTTTA54750.040.3561632
ACGACGG2100.039.64285727
TAAGCGA1250.039.62
AGGGGAA90350.038.923638
CGACGGT2200.038.86363628
GAGGGGA89100.038.7121247
TATGCGA1050.038.571432
AGACTTA9450.038.3333368
CGGTCTA2300.038.15217231
ATGGGCG950.037.8947375
GAGCGAT8050.037.732927
CTCACGA2150.037.67441624
TTATTCG301.1399984E-437.49999618
AATAGAG8200.037.0426833
GTTGCGA552.750312E-936.818182
CGTTAGA1350.036.6666642
Produced by FastQC (version 0.11.5)