##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546931_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1287971
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.709782285470713	31.0	31.0	34.0	30.0	34.0
2	32.059849173622695	33.0	31.0	34.0	30.0	34.0
3	32.056529223095865	33.0	31.0	34.0	30.0	34.0
4	35.829055933712795	37.0	35.0	37.0	35.0	37.0
5	35.828995373343034	37.0	35.0	37.0	35.0	37.0
6	35.875749531627655	37.0	35.0	37.0	35.0	37.0
7	36.06311555151475	37.0	35.0	37.0	35.0	37.0
8	13.204419975294474	0.0	0.0	35.0	0.0	37.0
9	24.879161875539122	18.0	17.0	38.0	17.0	39.0
10	32.63353677994303	32.0	32.0	37.0	27.0	39.0
11	35.53991898885922	37.0	35.0	37.0	32.0	39.0
12	36.61792074510994	37.0	35.0	39.0	33.0	39.0
13	36.85081729324651	39.0	35.0	39.0	33.0	39.0
14	38.000437121643266	40.0	37.0	41.0	33.0	41.0
15	38.29161293227875	40.0	37.0	41.0	33.0	41.0
16	38.391363625423246	40.0	37.0	41.0	34.0	41.0
17	38.372855444726625	40.0	37.0	41.0	34.0	41.0
18	38.293012808518206	40.0	37.0	41.0	34.0	41.0
19	38.25804307705686	40.0	37.0	41.0	34.0	41.0
20	38.20883389455197	40.0	37.0	41.0	34.0	41.0
21	38.12185289886185	40.0	37.0	41.0	34.0	41.0
22	38.082288343448724	40.0	36.0	41.0	33.0	41.0
23	37.96414670827216	40.0	36.0	41.0	33.0	41.0
24	37.82671737174206	40.0	35.0	41.0	33.0	41.0
25	37.716075129020766	40.0	35.0	41.0	33.0	41.0
26	37.638706927407526	40.0	35.0	41.0	33.0	41.0
27	37.58983082693632	40.0	35.0	41.0	33.0	41.0
28	37.50078612018438	40.0	35.0	41.0	33.0	41.0
29	37.48821440855423	40.0	35.0	41.0	33.0	41.0
30	37.37309923903566	40.0	35.0	41.0	33.0	41.0
31	37.27027704816335	40.0	35.0	41.0	33.0	41.0
32	37.08589867318441	40.0	35.0	41.0	32.0	41.0
33	36.86897608719451	40.0	35.0	41.0	31.0	41.0
34	36.63090783876345	40.0	35.0	41.0	31.0	41.0
35	36.443892758454965	40.0	35.0	41.0	30.0	41.0
36	36.31423844170404	40.0	35.0	41.0	30.0	41.0
37	36.244646036284976	39.0	35.0	41.0	30.0	41.0
38	36.154326456108095	39.0	35.0	41.0	29.0	41.0
39	36.06790913770574	39.0	35.0	41.0	29.0	41.0
40	35.94807569425088	39.0	35.0	41.0	28.0	41.0
41	35.8858755360175	39.0	35.0	41.0	28.0	41.0
42	35.82029331405754	39.0	35.0	41.0	28.0	41.0
43	35.7067340801928	39.0	35.0	40.0	27.0	41.0
44	35.5217143864264	38.0	35.0	40.0	27.0	41.0
45	35.43147788265419	38.0	35.0	40.0	26.0	41.0
46	35.39555626640662	38.0	35.0	40.0	26.0	41.0
47	35.36244837810789	38.0	35.0	40.0	26.0	41.0
48	35.286682697048306	38.0	35.0	40.0	26.0	41.0
49	35.18466021362282	38.0	34.0	40.0	26.0	41.0
50	35.08544524682621	38.0	34.0	40.0	26.0	41.0
51	34.711922861617225	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	1.0
12	4.0
13	8.0
14	14.0
15	27.0
16	54.0
17	165.0
18	381.0
19	822.0
20	1561.0
21	2511.0
22	4240.0
23	6456.0
24	9942.0
25	14969.0
26	19293.0
27	21018.0
28	21352.0
29	22421.0
30	26190.0
31	32809.0
32	42714.0
33	58946.0
34	89985.0
35	134595.0
36	140395.0
37	232228.0
38	285947.0
39	118901.0
40	20.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.323709151836496	23.20060001350962	27.86755291850515	16.60813791614873
2	34.596664055324226	23.467453847951543	29.13737964597029	12.798502450753938
3	29.599424210638283	24.16475215668676	32.794760130468774	13.441063502206182
4	26.503547051913433	26.526373652822926	31.735574791668448	15.23450450359519
5	23.009058433769084	30.939050646326667	31.360178140656892	14.691712779247359
6	21.515701828690244	39.551045792180105	28.830928646685365	10.102323732444287
7	83.63332714789385	4.377427752643499	9.791214243177835	2.1980308562848077
8	31.367709366127034	63.78854803407841	3.6513244475224984	1.192418152272062
9	79.4118035266322	5.699584850901146	11.164381806733225	3.7242298157334286
10	45.03595189643245	25.81828317563051	17.171815203913752	11.973949724023289
11	38.059552583093875	22.525351890686977	24.13198744381667	15.283108082402478
12	33.8718030141983	21.176175550536463	28.882870809979416	16.06915062528582
13	24.26584138928594	28.717649698634517	29.32830009371329	17.688208818366252
14	18.902366590552116	32.0852721062819	31.485258596660948	17.527102706505037
15	17.968727556753993	24.261726389802256	41.04906088724047	16.720485166203275
16	21.864389803807693	21.061731979990235	38.739536837397736	18.334341378804336
17	21.79994735906321	21.027880286124454	29.998501519055942	27.173670835756397
18	24.153494139231395	23.70658966700337	32.64297099857062	19.496945195194613
19	27.437186085711556	24.96989450849437	28.392331814924404	19.20058759086967
20	28.76539922094519	24.653660680248237	27.099057354552237	19.481882744254335
21	25.778453086288433	25.06081270463388	30.798519531883873	18.36221467719382
22	23.95807048450625	22.650820554189497	30.616838422604236	22.774270538700016
23	22.046614403585174	25.965491459046824	29.862939460593445	22.124954676774557
24	22.827299682989757	24.006596421813846	34.00550167666819	19.16060221852821
25	21.874172632768907	24.463982496500307	32.3612099961878	21.300634874542983
26	20.031429279075383	28.58410631916402	29.118435120045405	22.266029281715195
27	20.064038708946086	27.160704705307804	32.24280670915727	20.53244987658884
28	18.98940271170702	26.425206778724053	35.526343372638046	19.05904713693088
29	21.03859481308197	25.714243566043027	33.86737744871585	19.379784172159155
30	21.993197051796972	25.79747525371301	32.50702073260967	19.702306961880353
31	27.35690477502987	24.487585512406724	28.29077673332707	19.864732979236333
32	27.91506951631675	25.505543214870517	28.33169380366483	18.247693465147897
33	26.2644888743613	25.76750563483184	27.65706681283973	20.310938677967126
34	21.344347038869664	26.93787360119133	30.281970634431985	21.43580872550702
35	21.503201547239804	26.94975275064423	30.292374595390736	21.25467110672523
36	27.119942917969425	25.88521014836514	27.51669098139632	19.47815595226911
37	22.808044591066103	29.356173392102775	27.731292086545427	20.104489930285695
38	22.229460135360192	30.06449679379427	25.65779819576683	22.04824487507871
39	22.621549708805556	27.956685360151745	26.672106747745094	22.749658183297605
40	23.17971445009243	25.946935140620404	27.513274755409867	23.3600756538773
41	20.458768093380986	24.98255007294419	28.45646369367012	26.10221814000471
42	22.88079467627765	26.348652260027595	26.498655637432833	24.271897426261926
43	21.783797927127242	26.35261197651189	27.158918950814886	24.70467114554598
44	21.82952877044592	27.698139166176876	28.646763009415583	21.82556905396162
45	21.262745822693212	29.809910316303707	26.732201268506824	22.195142592496257
46	22.85222260439094	28.553205002286543	27.802256417264054	20.792315976058468
47	22.085668077930325	26.932205771713804	28.545363210817637	22.436762939538234
48	22.830793550475903	25.54187943672645	29.664643070379693	21.962683942417957
49	22.41269407463367	24.805449812146392	30.406585241437888	22.375270871782053
50	20.838124460876838	28.10956147304559	28.92347731431841	22.128836751759163
51	20.70442579840695	29.253143122011288	27.699459071671644	22.342972007910117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	936.0
1	1918.5
2	2901.0
3	14212.0
4	25523.0
5	17313.5
6	9104.0
7	8957.0
8	8810.0
9	8825.0
10	8840.0
11	8613.0
12	8386.0
13	8122.5
14	7859.0
15	7396.5
16	6934.0
17	6636.0
18	6338.0
19	6377.0
20	6416.0
21	6461.0
22	6506.0
23	7054.5
24	7603.0
25	8761.0
26	11519.5
27	13120.0
28	14406.0
29	15692.0
30	18081.5
31	20471.0
32	24166.5
33	27862.0
34	31084.0
35	34306.0
36	38263.0
37	42220.0
38	52068.0
39	61916.0
40	71082.5
41	80249.0
42	85384.5
43	90520.0
44	91949.5
45	93379.0
46	94336.0
47	95293.0
48	93578.5
49	91864.0
50	88104.5
51	84345.0
52	79648.0
53	74951.0
54	71335.0
55	67719.0
56	65777.5
57	63836.0
58	60530.0
59	57224.0
60	52446.5
61	47669.0
62	42270.0
63	36871.0
64	31306.5
65	25742.0
66	21343.5
67	16945.0
68	14289.0
69	11633.0
70	9779.5
71	7926.0
72	6399.0
73	4872.0
74	3827.0
75	2049.0
76	1316.0
77	970.0
78	624.0
79	453.0
80	282.0
81	213.5
82	145.0
83	107.5
84	70.0
85	51.0
86	32.0
87	19.5
88	7.0
89	9.0
90	11.0
91	5.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1287971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.127664359096727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.79594283791215	19.74783904010872
2	5.821511794092788	3.1584803602534435
3	2.8343253574221334	2.306658809418735
4	1.996555254619122	2.166475232867938
5	1.6106415348285714	2.1846471479824943
6	1.3661969455144434	2.2237039311803386
7	1.1749825443633237	2.231217246390001
8	1.0651890980299892	2.3116873864261223
9	0.9469262540651495	2.3119107833786514
>10	10.124185413981628	54.59888177452748
>50	0.23458107745309392	3.9153122693808764
>100	0.026667876821840162	1.3652738136632436
>500	0.0017205081717992607	0.3013543807532426
>1k	0.0	0.0
>5k	5.735027239330869E-4	1.1765578236687646
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9644	0.7487746230311086	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5481	0.4255530598126821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.552829993843029E-4	0.0	0.0	0.010248677959363992	0.0
2	1.552829993843029E-4	0.0	0.0	0.02880499638578819	0.0
3	1.552829993843029E-4	0.0	0.0	0.04472150382267924	0.0
4	1.552829993843029E-4	0.0	0.0	0.06250140725218192	0.0
5	1.552829993843029E-4	0.0	0.0	0.10691234507609255	0.0
6	1.552829993843029E-4	0.0	0.0	0.16514346984520614	0.0
7	1.552829993843029E-4	0.0	0.0	0.20512884218666413	0.0
8	1.552829993843029E-4	0.0	0.0	0.28929222785295633	0.0
9	1.552829993843029E-4	0.0	0.0	0.4061426848896443	0.0
10	1.552829993843029E-4	0.0	0.0	0.5750129467200736	0.0
11	1.552829993843029E-4	0.0	0.0	0.7604984894846235	0.0
12	1.552829993843029E-4	0.0	0.0	0.927350072323057	0.0
13	1.552829993843029E-4	0.0	0.0	1.0337965684009967	0.0
14	1.552829993843029E-4	0.0	0.0	1.0715303372513822	0.0
15	1.552829993843029E-4	0.0	0.0	1.1112827850937637	0.0
16	1.552829993843029E-4	0.0	0.0	1.1903218317803739	0.0
17	1.552829993843029E-4	0.0	0.0	1.2717677649574408	0.0
18	1.552829993843029E-4	0.0	0.0	1.3965376549627282	0.0
19	1.552829993843029E-4	0.0	0.0	1.4552346287299947	0.0
20	1.552829993843029E-4	0.0	0.0	1.5295375439353835	0.0
21	1.552829993843029E-4	0.0	0.0	1.6121480996078328	0.0
22	1.552829993843029E-4	0.0	0.0	1.701047616755346	0.0
23	1.552829993843029E-4	0.0	0.0	1.798332415869612	0.0
24	1.552829993843029E-4	0.0	0.0	1.874731651566689	0.0
25	1.552829993843029E-4	0.0	0.0	1.9463171142828526	0.0
26	1.552829993843029E-4	0.0	0.0	2.018290784497477	0.0
27	1.552829993843029E-4	0.0	0.0	2.095311152192091	0.0
28	1.552829993843029E-4	0.0	0.0	2.1781546323636167	0.0
29	1.552829993843029E-4	0.0	0.0	2.262162735030525	0.0
30	1.552829993843029E-4	0.0	0.0	2.3595251756444826	0.0
31	1.552829993843029E-4	0.0	0.0	2.453704314771062	0.0
32	1.552829993843029E-4	0.0	0.0	2.542681473418268	0.0
33	1.552829993843029E-4	0.0	0.0	2.641519102526377	0.0
34	1.552829993843029E-4	0.0	0.0	2.739968524136025	0.0
35	1.552829993843029E-4	0.0	0.0	2.8558096416767147	0.0
36	1.552829993843029E-4	0.0	0.0	2.9602374587626583	0.0
37	1.552829993843029E-4	0.0	0.0	3.0638888608516806	0.0
38	1.552829993843029E-4	0.0	0.0	3.199761485312946	0.0
39	1.552829993843029E-4	0.0	0.0	3.3853246695771877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	55	1.8189894E-12	45.0	1
CGCGATT	20	7.0336036E-4	45.0	12
CGTTTTT	7525	0.0	43.146175	1
TCACGAC	200	0.0	40.5	25
GTTTTTA	5475	0.0	40.356163	2
ACGACGG	210	0.0	39.642857	27
TAAGCGA	125	0.0	39.6	2
AGGGGAA	9035	0.0	38.92363	8
CGACGGT	220	0.0	38.863636	28
GAGGGGA	8910	0.0	38.712124	7
TATGCGA	105	0.0	38.57143	2
AGACTTA	945	0.0	38.333336	8
CGGTCTA	230	0.0	38.152172	31
ATGGGCG	95	0.0	37.894737	5
GAGCGAT	805	0.0	37.73292	7
CTCACGA	215	0.0	37.674416	24
TTATTCG	30	1.1399984E-4	37.499996	18
AATAGAG	820	0.0	37.042683	3
GTTGCGA	55	2.750312E-9	36.81818	2
CGTTAGA	135	0.0	36.666664	2
>>END_MODULE