##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546930_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1108954
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739547357239346	31.0	31.0	34.0	30.0	34.0
2	32.07647386636416	33.0	31.0	34.0	30.0	34.0
3	32.11666218797173	33.0	31.0	34.0	30.0	34.0
4	35.846185684888646	37.0	35.0	37.0	35.0	37.0
5	35.81085960283294	37.0	35.0	37.0	35.0	37.0
6	35.856987756029554	37.0	35.0	37.0	35.0	37.0
7	36.093727963468275	37.0	35.0	37.0	35.0	37.0
8	13.225985027332062	0.0	0.0	35.0	0.0	37.0
9	24.90948046537548	18.0	17.0	39.0	17.0	39.0
10	32.71405486611708	32.0	32.0	38.0	27.0	39.0
11	35.56972336093291	37.0	35.0	37.0	32.0	39.0
12	36.51072181533229	37.0	35.0	39.0	33.0	39.0
13	36.60404849975743	39.0	35.0	39.0	33.0	39.0
14	37.79132948706619	40.0	36.0	41.0	33.0	41.0
15	38.150198294969854	40.0	37.0	41.0	33.0	41.0
16	38.29143138489063	40.0	37.0	41.0	34.0	41.0
17	38.28795243084925	40.0	37.0	41.0	34.0	41.0
18	38.23387264034396	40.0	36.0	41.0	34.0	41.0
19	38.21641023883768	40.0	37.0	41.0	34.0	41.0
20	38.17562946704732	40.0	36.0	41.0	34.0	41.0
21	38.082851948773346	40.0	36.0	41.0	34.0	41.0
22	38.02332828954132	40.0	36.0	41.0	33.0	41.0
23	37.95374740521248	40.0	36.0	41.0	34.0	41.0
24	37.882963585504896	40.0	35.0	41.0	33.0	41.0
25	37.7860767894791	40.0	35.0	41.0	33.0	41.0
26	37.68287052483692	40.0	35.0	41.0	33.0	41.0
27	37.61108305664617	40.0	35.0	41.0	33.0	41.0
28	37.59586962128276	40.0	35.0	41.0	33.0	41.0
29	37.62365526433017	40.0	35.0	41.0	33.0	41.0
30	37.55721968629898	40.0	36.0	41.0	33.0	41.0
31	37.47860145686836	40.0	35.0	41.0	33.0	41.0
32	37.30668449728302	40.0	35.0	41.0	33.0	41.0
33	37.18649466073435	40.0	35.0	41.0	32.0	41.0
34	37.08165803090119	40.0	35.0	41.0	32.0	41.0
35	36.97902708317928	40.0	35.0	41.0	32.0	41.0
36	36.86252901382745	40.0	35.0	41.0	31.0	41.0
37	36.762566346304716	40.0	35.0	41.0	31.0	41.0
38	36.691177451905126	39.0	35.0	41.0	31.0	41.0
39	36.647447955460734	39.0	35.0	41.0	31.0	41.0
40	36.50289371786386	39.0	35.0	41.0	30.0	41.0
41	36.486959783724124	39.0	35.0	41.0	31.0	41.0
42	36.45150204607224	39.0	35.0	41.0	30.0	41.0
43	36.36052802911573	39.0	35.0	41.0	30.0	41.0
44	36.179012835518876	39.0	35.0	41.0	30.0	41.0
45	36.09093884868083	39.0	35.0	40.0	30.0	41.0
46	36.094483630520294	39.0	35.0	40.0	30.0	41.0
47	36.0478568092094	39.0	35.0	40.0	30.0	41.0
48	35.99178956025227	38.0	35.0	40.0	30.0	41.0
49	35.88132690805931	38.0	35.0	40.0	29.0	41.0
50	35.75362999727671	38.0	35.0	40.0	29.0	41.0
51	35.36827587077553	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	5.0
14	9.0
15	16.0
16	45.0
17	119.0
18	301.0
19	593.0
20	1032.0
21	1797.0
22	2808.0
23	4517.0
24	6564.0
25	9377.0
26	12070.0
27	13750.0
28	14702.0
29	17102.0
30	21412.0
31	27561.0
32	37190.0
33	51334.0
34	81552.0
35	128386.0
36	112950.0
37	192436.0
38	270430.0
39	100858.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.43461135448359	24.047616041783517	27.664357583813214	13.853415019919671
2	30.136507014718372	26.05888071101236	30.63165830142639	13.172953972842876
3	30.495854652221823	25.741193953942183	30.545811638715403	13.217139755120591
4	28.09268914670942	26.983355486341182	30.08005742348195	14.843897943467448
5	24.779837576671348	32.24173410258676	28.928431657219328	14.049996663522563
6	21.9820659828992	42.10841928520029	25.77645240469848	10.13306232720203
7	86.72397592686441	4.942134660229369	6.6000032463023715	1.7338861666038448
8	32.706316041963866	63.94007325822352	2.2827817925720995	1.0708289072405168
9	83.19100702103063	6.355177942457487	7.544406711189103	2.9094083253227816
10	52.3702516064688	25.33766053416102	12.0417979465334	10.25028991283678
11	46.881385521852124	20.90203921893965	18.946683090551996	13.26989216865623
12	41.743300443480976	22.115344730259327	22.2391550957028	13.902199730556902
13	22.408503869412076	37.98759912494116	23.49835971555177	16.10553729009499
14	15.590998364224303	42.60952212625591	26.53617733467754	15.263302174842238
15	14.532974316337738	24.658822638269935	46.00948280992719	14.798720235465131
16	17.20278749163626	19.319015937541142	44.86038194550901	18.617814625313585
17	17.448875246403368	19.37420307785535	28.02451679690952	35.15240487883177
18	21.653648392990153	25.791150940435763	31.90042147825789	20.654779188316198
19	29.34648326260602	25.416563716799796	24.842509247453005	20.394443773141177
20	32.13794260176707	23.879349368864712	23.820284700717977	20.16242332865024
21	23.972229686713785	27.59230770618078	26.99237299292847	21.443089614176962
22	22.83268737927813	25.081833872279645	25.796471269322264	26.289007479119963
23	21.453099046488852	30.484763119119457	24.77018884462295	23.291948989768738
24	21.17887667116941	25.524773795847256	35.00947739942324	18.286872133560095
25	19.70271084283027	25.99260203759579	32.52623643541572	21.778450684158223
26	17.532918407796895	35.215347074811035	26.155007331232856	21.09672718615921
27	20.28046248987785	32.578988848951354	28.758632008180683	18.381916652990114
28	15.92212120610954	30.041823195551842	36.60557606537331	17.430479532965297
29	17.339222366301936	27.52945568526738	34.747338482930765	20.383983465499924
30	19.646261251593845	29.00517063827715	30.950968209682276	20.39759990044673
31	30.165543385929443	27.018343411899863	23.124854592706278	19.691258609464416
32	29.77797095280778	28.55519705957145	24.750981555592027	16.91585043202874
33	26.924020292996826	28.700919966022038	24.09739267814535	20.27766706283579
34	20.635662074351146	29.13430133260712	27.174436450925825	23.055600142115903
35	20.992574985076025	26.569992984379876	28.935375137291537	23.50205689325256
36	30.027034484748693	26.452855573810997	25.01393204767736	18.506177893762953
37	22.12860046494264	32.65347345336236	26.414711520946767	18.803214560748234
38	21.440654887398395	33.50797237757382	22.03734329827928	23.014029436748505
39	22.174138873208445	29.70420774892376	25.87934215485944	22.24231122300835
40	23.692416457310223	26.517511096041858	25.654084840308975	24.135987606338947
41	17.715162215925996	26.22065477918832	27.702952512006814	28.361230492878875
42	23.203126549883947	27.605202740600603	23.29077671391239	25.900893995603063
43	23.836606387640966	27.484638677528554	24.262232698560986	24.416522236269493
44	21.32351747682952	31.34350027142695	26.769370055024826	20.563612196718708
45	19.362390144225998	35.42184797565995	22.521493226950803	22.694268653163252
46	23.24604988123944	31.791489998683442	24.821227931907007	20.141232188170115
47	23.569417667459604	27.79664440544874	25.85382261121742	22.780115315874237
48	24.360703870494177	24.700573693769083	28.886319901456687	22.052402534280052
49	21.039285669198183	25.46165124973624	30.018377678424894	23.480685402640685
50	19.57303909810506	31.857227621704777	26.74826908961057	21.821464190579594
51	19.45013048332032	32.71380057243132	24.28982626871809	23.546242675530273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	990.0
1	1557.5
2	2125.0
3	5539.5
4	8954.0
5	6589.0
6	4224.0
7	4348.0
8	4472.0
9	4708.0
10	4944.0
11	4951.0
12	4958.0
13	4668.5
14	4379.0
15	4109.0
16	3839.0
17	3806.5
18	3774.0
19	3818.0
20	3862.0
21	4140.0
22	4418.0
23	4713.0
24	5008.0
25	6749.5
26	10802.0
27	13113.0
28	14973.5
29	16834.0
30	17994.0
31	19154.0
32	20750.0
33	22346.0
34	23577.0
35	24808.0
36	30086.0
37	35364.0
38	46814.5
39	58265.0
40	74705.5
41	91146.0
42	97493.0
43	103840.0
44	100542.0
45	97244.0
46	95267.5
47	93291.0
48	88601.0
49	83911.0
50	79548.0
51	75185.0
52	69373.5
53	63562.0
54	58655.0
55	53748.0
56	51904.5
57	50061.0
58	47569.0
59	45077.0
60	40057.0
61	35037.0
62	29103.5
63	23170.0
64	18859.0
65	14548.0
66	12018.5
67	9489.0
68	7923.0
69	6357.0
70	5171.5
71	3986.0
72	3273.0
73	2560.0
74	1976.0
75	1009.5
76	627.0
77	444.5
78	262.0
79	165.0
80	68.0
81	49.0
82	30.0
83	23.0
84	16.0
85	15.5
86	15.0
87	11.5
88	8.0
89	4.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1108954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.843920804359676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06032566010373	16.83299650877472
2	5.613775867946807	2.4525375094571116
3	2.002656345273587	1.3123759981351388
4	1.1961936413966485	1.0451823667738804
5	0.8980624600779492	0.9808602627655572
6	0.7307183377094967	0.9577052099531757
7	0.6300886865751169	0.9634525158488902
8	0.5630904931830574	0.984008331102283
9	0.49874253184992295	0.9805043130745996
>10	9.41795730146301	50.404764974462054
>50	1.2756367026442217	18.035483865498897
>100	0.1065110877297286	3.6557921895834227
>500	0.0045766483008867756	0.6926237980563148
>1k	0.0016642357457770094	0.7017121565139492
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3070	0.27683745222975886	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1812	0.16339721936166873	No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1598	0.14409975526487123	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1241	0.11190725674825104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8035013174577124E-4	0.0	0.0	0.014969060934899013	0.0
2	1.8035013174577124E-4	0.0	0.0	0.04544823319993435	0.0
3	1.8035013174577124E-4	0.0	0.0	0.06411447183562168	0.0
4	1.8035013174577124E-4	0.0	0.0	0.11199743181412394	0.0
5	1.8035013174577124E-4	0.0	0.0	0.21605945783143393	0.0
6	1.8035013174577124E-4	0.0	0.0	0.30443102238686187	0.0
7	1.8035013174577124E-4	0.0	0.0	0.3643974411923308	0.0
8	1.8035013174577124E-4	0.0	0.0	0.49497093657626917	0.0
9	1.8035013174577124E-4	0.0	0.0	0.629151434595123	0.0
10	1.8035013174577124E-4	0.0	0.0	0.9155474438074077	0.0
11	1.8035013174577124E-4	0.0	0.0	1.1718249810181487	0.0
12	1.8035013174577124E-4	0.0	0.0	1.4257579665161946	0.0
13	1.8035013174577124E-4	0.0	0.0	1.5193596848922497	0.0
14	1.8035013174577124E-4	0.0	0.0	1.553986910187438	0.0
15	1.8035013174577124E-4	0.0	0.0	1.6124203528730678	0.0
16	1.8035013174577124E-4	0.0	0.0	1.7284756626514715	0.0
17	1.8035013174577124E-4	0.0	0.0	1.8663533383711137	0.0
18	1.8035013174577124E-4	0.0	0.0	2.0752889659985896	0.0
19	1.8035013174577124E-4	0.0	0.0	2.1659149072008397	0.0
20	1.8035013174577124E-4	0.0	0.0	2.2628531030141916	0.0
21	1.8035013174577124E-4	0.0	0.0	2.389639245631469	0.0
22	1.8035013174577124E-4	0.0	0.0	2.5254428948360346	0.0
23	1.8035013174577124E-4	0.0	0.0	2.680724358269144	0.0
24	1.8035013174577124E-4	0.0	0.0	2.7918200394245387	0.0
25	1.8035013174577124E-4	0.0	0.0	2.8819049302315514	0.0
26	1.8035013174577124E-4	0.0	0.0	2.9716291207750727	0.0
27	1.8035013174577124E-4	0.0	0.0	3.0578364837495515	0.0
28	1.8035013174577124E-4	0.0	0.0	3.1571192312755985	0.0
29	1.8035013174577124E-4	0.0	0.0	3.2564921538675184	0.0
30	1.8035013174577124E-4	0.0	0.0	3.3881477500419312	0.0
31	1.8035013174577124E-4	0.0	0.0	3.523320173785387	0.0
32	1.8035013174577124E-4	0.0	0.0	3.6378425074439518	0.0
33	1.8035013174577124E-4	0.0	0.0	3.754529042683466	0.0
34	1.8035013174577124E-4	0.0	0.0	3.872387853779327	0.0
35	1.8035013174577124E-4	0.0	0.0	4.034883322482267	0.0
36	1.8035013174577124E-4	0.0	0.0	4.178532202417774	0.0
37	1.8035013174577124E-4	0.0	0.0	4.306670971023144	0.0
38	1.8035013174577124E-4	0.0	0.0	4.440490768778506	0.0
39	1.8035013174577124E-4	0.0	0.0	4.568359012186258	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	25	3.8907157E-5	45.000004	45
CTATACG	25	3.8907157E-5	45.000004	1
AAACGCG	25	3.8907157E-5	45.000004	1
CGCTAAC	25	3.8907157E-5	45.000004	14
AACGATC	25	3.8907157E-5	45.000004	42
TCGTAAG	30	2.1654323E-6	45.000004	1
ATACGTT	25	3.8907157E-5	45.000004	10
GTGCGTT	25	3.8907157E-5	45.000004	44
GGTACGA	35	1.2118835E-7	45.0	2
CGACGGC	20	7.0331804E-4	45.0	15
TTCACGT	20	7.0331804E-4	45.0	26
TTCACGA	35	1.2118835E-7	45.0	2
ATTCGCG	20	7.0331804E-4	45.0	1
TTTCACG	20	7.0331804E-4	45.0	25
CATACGC	20	7.0331804E-4	45.0	32
TCGTAAA	20	7.0331804E-4	45.0	38
ACGTCGA	20	7.0331804E-4	45.0	2
GTTCACG	45	3.8562575E-10	45.0	1
ATCGGCA	20	7.0331804E-4	45.0	14
TCGACAC	35	1.2118835E-7	45.0	34
>>END_MODULE