FastQCFastQC Report
Thu 26 May 2016
SRR1546929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546929_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences858899
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.38817136822839476No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17100.19909209348247No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8610.1002446154902963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.0322887E-445.0000041
TCGGTAT207.0322887E-445.00000422
TAGTACG700.045.0000041
GTAGCGC253.8899794E-545.034
ACGGTCT1350.045.030
CGGTCTA1450.043.4482831
CGTTTTT31850.042.315541
TTAACGA800.042.1875042
TTTAGCG700.041.7857171
TTAGCGA1050.040.7142872
CACGACG1500.040.50000426
CTTGCGA501.0804797E-940.52
TTCGCAT501.0804797E-940.525
AGGGGAA72600.040.2272728
ACGGGCG451.9274012E-840.05
CGAGGTA451.9274012E-840.06
AGTACGA900.040.02
GAGGGGA73200.039.9897547
GAGCGAT5650.039.424787
CGAATGA800.039.3750042