##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546929_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	858899
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71742544816096	31.0	31.0	34.0	30.0	34.0
2	32.06252772444723	33.0	31.0	34.0	30.0	34.0
3	32.10023297267781	33.0	31.0	34.0	30.0	34.0
4	35.84451256783394	37.0	35.0	37.0	35.0	37.0
5	35.82791690291874	37.0	35.0	37.0	35.0	37.0
6	35.8758713189793	37.0	35.0	37.0	35.0	37.0
7	36.10141937526997	37.0	35.0	37.0	35.0	37.0
8	13.282605987432747	0.0	0.0	35.0	0.0	37.0
9	24.953785020124602	18.0	17.0	39.0	17.0	39.0
10	32.64851396962856	32.0	32.0	37.0	27.0	39.0
11	35.541547958491044	37.0	35.0	37.0	32.0	39.0
12	36.566557883988686	37.0	35.0	39.0	33.0	39.0
13	36.75602602867159	39.0	35.0	39.0	33.0	39.0
14	37.935518611617894	40.0	37.0	41.0	33.0	41.0
15	38.24553643676381	40.0	37.0	41.0	33.0	41.0
16	38.34390772372537	40.0	37.0	41.0	34.0	41.0
17	38.32613846331175	40.0	37.0	41.0	34.0	41.0
18	38.26242084342862	40.0	37.0	41.0	34.0	41.0
19	38.2516081634744	40.0	37.0	41.0	34.0	41.0
20	38.22015277698542	40.0	37.0	41.0	34.0	41.0
21	38.14489596564905	40.0	37.0	41.0	34.0	41.0
22	38.09117952168998	40.0	36.0	41.0	33.0	41.0
23	37.991812774261	40.0	36.0	41.0	33.0	41.0
24	37.904313545597326	40.0	36.0	41.0	33.0	41.0
25	37.79633460977367	40.0	35.0	41.0	33.0	41.0
26	37.72681421214834	40.0	35.0	41.0	33.0	41.0
27	37.67345753109504	40.0	35.0	41.0	33.0	41.0
28	37.60515730021807	40.0	36.0	41.0	33.0	41.0
29	37.61748820292025	40.0	36.0	41.0	33.0	41.0
30	37.52921822007011	40.0	36.0	41.0	33.0	41.0
31	37.46646578934194	40.0	35.0	41.0	33.0	41.0
32	37.322005264879806	40.0	35.0	41.0	33.0	41.0
33	37.18989660018233	40.0	35.0	41.0	32.0	41.0
34	37.025075125247554	40.0	35.0	41.0	31.0	41.0
35	36.896517518357804	40.0	35.0	41.0	31.0	41.0
36	36.79376504105838	40.0	35.0	41.0	31.0	41.0
37	36.72091596334377	40.0	35.0	41.0	31.0	41.0
38	36.63959673954679	40.0	35.0	41.0	31.0	41.0
39	36.56389167992977	39.0	35.0	41.0	30.0	41.0
40	36.4500808593327	39.0	35.0	41.0	30.0	41.0
41	36.39847525727705	39.0	35.0	41.0	30.0	41.0
42	36.345672774098006	39.0	35.0	41.0	30.0	41.0
43	36.24324396698564	39.0	35.0	41.0	30.0	41.0
44	36.077266360771176	39.0	35.0	40.0	29.0	41.0
45	35.987084628111106	39.0	35.0	40.0	29.0	41.0
46	35.94669687588413	38.0	35.0	40.0	29.0	41.0
47	35.91050519327651	38.0	35.0	40.0	29.0	41.0
48	35.834293671316416	38.0	35.0	40.0	29.0	41.0
49	35.74918005493079	38.0	35.0	40.0	29.0	41.0
50	35.62923580071696	38.0	35.0	40.0	28.0	41.0
51	35.26049512224371	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	8.0
15	15.0
16	37.0
17	84.0
18	227.0
19	454.0
20	837.0
21	1512.0
22	2364.0
23	3631.0
24	5349.0
25	7934.0
26	9963.0
27	11400.0
28	11928.0
29	13730.0
30	16438.0
31	21066.0
32	28252.0
33	39076.0
34	60714.0
35	91609.0
36	92256.0
37	156551.0
38	201318.0
39	82126.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.37633644933805	22.250695366975627	28.005504721742604	14.367463461943721
2	32.80816487153903	24.943328610232403	29.367830210536976	12.88067630769159
3	30.165362865715295	24.847042550986785	31.49543776392801	13.492156819369914
4	27.020639213691016	26.14207258362159	31.62118013875904	15.216108063928354
5	23.85134922732475	31.428840876517494	29.78883430997125	14.930975586186502
6	21.948331526756927	40.99876702615791	27.150922285390948	9.901979161694216
7	85.98624518133097	4.3639589753859305	7.59111373979944	2.0586821034836458
8	32.54282517502058	63.70632635501963	2.7259316869620296	1.0249167829977681
9	82.89065419799068	5.039591383853049	8.608113410307848	3.46164100784842
10	47.34875695512511	24.99933053828215	15.28282137946371	12.369091127129034
11	40.89863883879246	22.555737054065727	21.527676711697186	15.017947395444633
12	36.452248751017294	22.41474259488019	25.30437222537225	15.82863642873027
13	23.49088775280912	32.2554805629067	26.610695786116878	17.642935898167305
14	18.473301284551503	35.1786414933537	28.79581883318062	17.55223838891418
15	17.339524204825015	24.573669313854133	41.13976148534345	16.947044995977407
16	20.328816310183154	21.56586513664587	39.74867824971272	18.356640303458267
17	21.13694392472223	21.35594522755295	28.234518843309864	29.272592004414953
18	23.891167646021245	24.17536869876435	31.50545058266455	20.42801307254986
19	28.00969613423697	25.57949188437756	26.10202130867541	20.30879067271006
20	30.525475055856393	24.83865972599805	25.136366441223007	19.49949877692255
21	25.75506549664163	25.919345580795877	28.54747764288933	19.778111279673162
22	24.110751089476178	22.928772766064462	28.52593843979327	24.43453770466609
23	22.18782417956011	27.52814940988405	27.27107611022949	23.01295030032635
24	23.0796636158617	24.459569751507455	33.20716405537787	19.25360257725297
25	20.790453825187825	24.75005792299211	32.233359219186426	22.22612903263364
26	19.89279298264406	31.094692158216507	27.18841214159057	21.82410271754886
27	20.43197162879454	29.328244648090173	30.087705306444647	20.152078416670644
28	18.355708878459517	27.191672129086193	35.72899723948916	18.723621752965133
29	19.771707732806767	25.59567539373081	34.062212204228906	20.57040466923352
30	21.347562402564215	26.542468904958554	32.32720028781033	19.782768404666907
31	27.868352390676904	24.940185050861626	26.42126722699642	20.77019533146505
32	28.40846246182613	26.386920930167573	26.789063673377196	18.4155529346291
33	26.628858573592474	25.934015524526167	26.827019242076194	20.61010665980517
34	21.758087970762567	27.318113072666282	28.59055604908144	22.333242907489705
35	21.369916602534175	26.76950374840348	29.36398808241714	22.496591566645204
36	27.794420531401247	25.969060390104076	26.530243951849986	19.706275126644694
37	22.241963257612362	30.081767472077626	27.553880025474477	20.12238924483554
38	21.959625054866756	31.363873982854795	24.28364685486885	22.3928541074096
39	22.02214695790774	28.479600046105535	27.106213885451027	22.3920391105357
40	23.71221761813671	25.789062509095945	27.04369198241004	23.455027890357304
41	20.13414848544474	25.05277104758534	28.288425065112428	26.524655401857494
42	22.811063931847634	26.16826891171139	25.623036003069043	25.397631153371936
43	22.81746747871403	26.257336427216703	25.72619132168043	25.19900477238884
44	21.03518574360897	28.367596190006044	28.652728667747894	21.944489398637092
45	21.24475636832736	31.55283682947588	25.232303216094092	21.970103586102674
46	22.83225385056916	29.780335056857677	26.931105985686326	20.45630510688684
47	22.723626410090127	27.657501056585232	26.74994382342976	22.868928709894874
48	23.10679136895025	25.453865937671367	29.26467489192559	22.17466780145279
49	22.137876514002226	24.85437752285193	30.181779231318234	22.825966731827606
50	20.713261978416554	29.281440541903063	28.137301359065503	21.86799612061488
51	20.304599260215696	30.903750033473088	26.367943145818078	22.423707560493142
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	1101.0
2	1622.0
3	5395.5
4	9169.0
5	6519.5
6	3870.0
7	3935.0
8	4000.0
9	4138.5
10	4277.0
11	4085.5
12	3894.0
13	3754.0
14	3614.0
15	3546.5
16	3479.0
17	3298.5
18	3118.0
19	3168.5
20	3219.0
21	3152.5
22	3086.0
23	3667.0
24	4248.0
25	5015.5
26	6850.5
27	7918.0
28	9565.5
29	11213.0
30	12462.0
31	13711.0
32	15612.5
33	17514.0
34	20177.5
35	22841.0
36	26561.0
37	30281.0
38	37742.5
39	45204.0
40	52939.0
41	60674.0
42	65025.0
43	69376.0
44	68713.0
45	68050.0
46	68906.0
47	69762.0
48	68086.0
49	66410.0
50	62465.5
51	58521.0
52	53971.5
53	49422.0
54	47198.0
55	44974.0
56	43011.5
57	41049.0
58	38737.0
59	36425.0
60	32994.5
61	29564.0
62	25833.0
63	22102.0
64	18562.0
65	15022.0
66	12033.5
67	9045.0
68	7497.0
69	5949.0
70	5127.0
71	4305.0
72	3514.0
73	2723.0
74	2114.5
75	1128.0
76	750.0
77	568.5
78	387.0
79	267.5
80	148.0
81	97.0
82	46.0
83	33.0
84	20.0
85	15.5
86	11.0
87	10.0
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	858899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.054874732213403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97668407689204	18.492886125313078
2	5.7513217309034	2.996999344866897
3	2.813131067154416	2.198873326800183
4	2.0796409460490226	2.167391373491562
5	1.674488280563494	2.1814291195320625
6	1.4415777213619327	2.253607616810088
7	1.3416887073196802	2.447027183918772
8	1.2032575161219077	2.508057908252044
9	1.081877501617685	2.5369364502223903
>10	11.449767282980392	57.68499726254608
>50	0.15824322782320022	2.5351810737919322
>100	0.02652372272316131	1.246272709245596
>500	8.991092448529257E-4	0.15953227725493438
>1k	8.991092448529257E-4	0.5908082279543972
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3334	0.38817136822839476	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1710	0.19909209348247	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	861	0.1002446154902963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009081393737796878	0.0
2	0.0	0.0	0.0	0.029107031210887428	0.0
3	0.0	0.0	0.0	0.04296197806726984	0.0
4	0.0	0.0	0.0	0.06624760303597978	0.0
5	0.0	0.0	0.0	0.13121449669868052	0.0
6	0.0	0.0	0.0	0.18919570287076828	0.0
7	0.0	0.0	0.0	0.22738412781945258	0.0
8	0.0	0.0	0.0	0.3098152402086858	0.0
9	0.0	0.0	0.0	0.39236278072276254	0.0
10	0.0	0.0	0.0	0.5713128086072984	0.0
11	0.0	0.0	0.0	0.7345450396379551	0.0
12	0.0	0.0	0.0	0.8903258706786246	0.0
13	0.0	0.0	0.0	0.9513342080966447	0.0
14	0.0	0.0	0.0	0.9754348299392595	0.0
15	0.0	0.0	0.0	1.0181639517568422	0.0
16	0.0	0.0	0.0	1.1008279203957625	0.0
17	0.0	0.0	0.0	1.193271851521541	0.0
18	0.0	0.0	0.0	1.3178499451041392	0.0
19	0.0	0.0	0.0	1.3851454012637108	0.0
20	0.0	0.0	0.0	1.4543037074207794	0.0
21	0.0	0.0	0.0	1.5559454604091982	0.0
22	0.0	0.0	0.0	1.6543272259019979	0.0
23	0.0	0.0	0.0	1.7699403538716427	0.0
24	0.0	0.0	0.0	1.8547000287577469	0.0
25	0.0	0.0	0.0	1.92991259740668	0.0
26	0.0	0.0	0.0	2.0060565910543615	0.0
27	0.0	0.0	0.0	2.0818513003275125	0.0
28	0.0	1.1642812484354971E-4	0.0	2.1674259720875213	0.0
29	0.0	1.1642812484354971E-4	0.0	2.2566099157176804	0.0
30	0.0	1.1642812484354971E-4	0.0	2.367216634319053	0.0
31	0.0	1.1642812484354971E-4	0.0	2.4730497998018395	0.0
32	0.0	1.1642812484354971E-4	0.0	2.5686372902983936	0.0
33	0.0	1.1642812484354971E-4	0.0	2.6677176245402543	0.0
34	0.0	1.1642812484354971E-4	0.0	2.7732015056485104	0.0
35	0.0	1.1642812484354971E-4	0.0	2.8994095929789183	0.0
36	0.0	1.1642812484354971E-4	0.0	3.002914195964834	0.0
37	0.0	1.1642812484354971E-4	0.0	3.1178287551854176	0.0
38	0.0	1.1642812484354971E-4	0.0	3.23565401752709	0.0
39	0.0	1.1642812484354971E-4	0.0	3.392133417316821	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.0322887E-4	45.000004	1
TCGGTAT	20	7.0322887E-4	45.000004	22
TAGTACG	70	0.0	45.000004	1
GTAGCGC	25	3.8899794E-5	45.0	34
ACGGTCT	135	0.0	45.0	30
CGGTCTA	145	0.0	43.44828	31
CGTTTTT	3185	0.0	42.31554	1
TTAACGA	80	0.0	42.187504	2
TTTAGCG	70	0.0	41.785717	1
TTAGCGA	105	0.0	40.714287	2
CACGACG	150	0.0	40.500004	26
CTTGCGA	50	1.0804797E-9	40.5	2
TTCGCAT	50	1.0804797E-9	40.5	25
AGGGGAA	7260	0.0	40.227272	8
ACGGGCG	45	1.9274012E-8	40.0	5
CGAGGTA	45	1.9274012E-8	40.0	6
AGTACGA	90	0.0	40.0	2
GAGGGGA	7320	0.0	39.989754	7
GAGCGAT	565	0.0	39.42478	7
CGAATGA	80	0.0	39.375004	2
>>END_MODULE