FastQCFastQC Report
Thu 26 May 2016
SRR1546928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546928_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences909746
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28240.3104163140041286No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15490.17026730537974336No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9540.10486443468836355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA302.1650012E-645.0000042
TAGCGTG302.1650012E-645.0000041
TCTAGCG302.1650012E-645.0000041
GACGATT302.1650012E-645.0000047
CACGTCA351.2115561E-745.00000434
GTCGCCC207.0325093E-445.016
CGAACTG207.0325093E-445.012
ACGCATG207.0325093E-445.01
AAACGCC207.0325093E-445.044
CTAACGT207.0325093E-445.031
TATTCGG253.8901628E-545.034
TCGTATA207.0325093E-445.015
ACGCTAA253.8901628E-545.039
CTACCGA207.0325093E-445.02
CGGTCTA800.045.031
CCGGTAC207.0325093E-445.020
AATAGAC253.8901628E-545.027
ACGACTA207.0325093E-445.021
TACGTAG650.044.9999961
CGTAAGA1000.042.752