FastQCFastQC Report
Thu 26 May 2016
SRR1546927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546927_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1077085
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99410.9229540844037378No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.5184363351081855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGG207.03309E-445.01
CTATACG351.211829E-745.01
CGCATCG351.211829E-745.021
CGCGTAG207.03309E-445.01
TCGAACA207.03309E-445.041
TAGTACG406.8139343E-945.01
TATAGCG302.165374E-644.9999961
CGTTTTT82700.043.938941
GCGTAAG900.042.51
TCACGAC2450.042.244925
TACGCGA650.041.538462
TTACGAG1200.041.2499963
CGTAAGA1050.040.7142872
ACGACGG2500.040.50000427
GTTTTTA59300.040.4089362
TAACGGG900.040.03
TGGTTCG451.9281288E-840.037
TATACGA451.9281288E-840.02
ACGTAGA850.039.7058832
CGGTCTA2500.039.60000231