##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546927_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1077085
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731315541484655	31.0	31.0	34.0	30.0	34.0
2	32.091701212067754	33.0	31.0	34.0	30.0	34.0
3	32.05901391255101	33.0	31.0	34.0	30.0	34.0
4	35.83557008035577	37.0	35.0	37.0	35.0	37.0
5	35.83942400089129	37.0	35.0	37.0	35.0	37.0
6	35.890492393822214	37.0	35.0	37.0	35.0	37.0
7	36.109566097383215	37.0	35.0	37.0	35.0	37.0
8	13.249518840202954	0.0	0.0	36.0	0.0	37.0
9	24.926331719409333	18.0	17.0	39.0	17.0	39.0
10	32.6608150703055	32.0	32.0	38.0	27.0	39.0
11	35.56806751556284	37.0	35.0	37.0	32.0	39.0
12	36.66418342099277	37.0	35.0	39.0	33.0	39.0
13	36.90555527186805	39.0	37.0	39.0	33.0	39.0
14	38.120332192909565	40.0	37.0	41.0	33.0	41.0
15	38.40186800484641	40.0	38.0	41.0	34.0	41.0
16	38.47735044123723	40.0	38.0	41.0	34.0	41.0
17	38.455573144180825	40.0	38.0	41.0	34.0	41.0
18	38.369128713147056	40.0	37.0	41.0	34.0	41.0
19	38.32816165855063	40.0	37.0	41.0	34.0	41.0
20	38.273578222702945	40.0	37.0	41.0	34.0	41.0
21	38.202460344355366	40.0	37.0	41.0	34.0	41.0
22	38.14243536953908	40.0	37.0	41.0	34.0	41.0
23	38.00250769437881	40.0	36.0	41.0	34.0	41.0
24	37.81007998440234	40.0	35.0	41.0	33.0	41.0
25	37.6872410255458	40.0	35.0	41.0	33.0	41.0
26	37.614928255430165	40.0	35.0	41.0	33.0	41.0
27	37.55708695228324	40.0	35.0	41.0	33.0	41.0
28	37.4720955170669	40.0	35.0	41.0	33.0	41.0
29	37.445321399889515	40.0	35.0	41.0	33.0	41.0
30	37.31677815585585	40.0	35.0	41.0	33.0	41.0
31	37.19825361972361	40.0	35.0	41.0	32.0	41.0
32	36.96743618191693	40.0	35.0	41.0	31.0	41.0
33	36.70836006443317	40.0	35.0	41.0	31.0	41.0
34	36.41494960936231	40.0	35.0	41.0	30.0	41.0
35	36.19614050887349	40.0	35.0	41.0	29.0	41.0
36	36.04757099021897	39.0	35.0	41.0	28.0	41.0
37	35.968724845300045	39.0	35.0	41.0	27.0	41.0
38	35.86980971789599	39.0	35.0	41.0	27.0	41.0
39	35.78458896001708	39.0	35.0	41.0	27.0	41.0
40	35.666990998853386	39.0	35.0	41.0	26.0	41.0
41	35.60855271403835	39.0	35.0	41.0	26.0	41.0
42	35.538314060635884	39.0	35.0	41.0	25.0	41.0
43	35.4483471592307	39.0	35.0	41.0	25.0	41.0
44	35.263269844069875	38.0	35.0	40.0	24.0	41.0
45	35.17717821713235	38.0	35.0	40.0	24.0	41.0
46	35.14909036891239	38.0	35.0	40.0	24.0	41.0
47	35.11639564194098	38.0	35.0	40.0	24.0	41.0
48	35.01999656480222	38.0	34.0	40.0	23.0	41.0
49	34.93090610304665	38.0	34.0	40.0	24.0	41.0
50	34.810444858112405	38.0	34.0	40.0	24.0	41.0
51	34.44954205099876	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	7.0
14	22.0
15	27.0
16	53.0
17	146.0
18	360.0
19	772.0
20	1427.0
21	2362.0
22	3761.0
23	6100.0
24	9633.0
25	14542.0
26	18961.0
27	19887.0
28	18910.0
29	19338.0
30	21634.0
31	27133.0
32	34707.0
33	47623.0
34	72298.0
35	109288.0
36	113026.0
37	190481.0
38	241660.0
39	102902.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.39419358732133	22.314951930441886	27.623910833406836	17.666943648829943
2	35.86151510790699	23.598508938477465	27.743028637479867	12.796947316135682
3	28.567847477218606	23.75578529085448	34.738483963661174	12.937883268265734
4	25.878273302478448	26.167294131846603	33.44239312589071	14.512039439784234
5	22.736645668633397	30.22741937730077	32.57774456055	14.458190393515832
6	20.962969496372153	38.63789765895913	30.366034249850287	10.033098594818423
7	81.95787704777247	4.473834469888635	11.524160117353784	2.044128364985122
8	30.875650482552448	63.803599530213496	4.193726586109731	1.127023401124331
9	77.8925525840579	5.700292920243063	12.94224689787714	3.464907597821899
10	43.217387671353706	25.277392220669675	19.612936769150068	11.892283338826555
11	36.20995557453683	22.62458394648519	25.907054689277075	15.258405789700905
12	31.85198939730848	21.963076266032857	30.191489065394094	15.99344527126457
13	22.635725128471755	29.516704809741107	30.968586508957046	16.878983552830093
14	17.889024543095484	32.281946178806685	33.02283478091329	16.80619449718453
15	17.33827878022626	24.50038762028995	42.064461022110606	16.09687257737319
16	20.66800670327783	21.715370653198214	40.15616223417836	17.460460409345597
17	20.73615359976232	21.614542956219797	31.46483332327532	26.18447012074256
18	22.755585678010558	23.961804314422725	33.920999735396926	19.36161027216979
19	26.167479818213046	25.199775319496602	29.911009808882305	18.721735053408043
20	27.25513770965151	24.758213140095723	29.217378387035374	18.76927076321739
21	24.411536693947088	25.380819526778296	32.09690971464648	18.11073406462814
22	22.843972388437308	22.873125147968825	31.839269881207148	22.44363258238672
23	21.224880116239667	25.847913581565056	30.747712576073383	22.179493726121894
24	21.21308903197055	24.3933394300357	35.56460260796502	18.828968930028736
25	20.529856046644415	24.672518881982388	34.093966585738364	20.703658485634836
26	19.463273557797205	29.409099560387524	30.687085977429824	20.440540904385447
27	19.75656517359354	28.16500090522104	32.43773704025216	19.64069688093326
28	18.316381715463496	26.345831573181318	36.6896763022417	18.648110409113485
29	19.40589647056639	25.061624662863192	35.773592613396346	19.758886253174076
30	20.648509634801336	26.219193471267353	33.74552611910852	19.386770774822786
31	26.25317407632638	24.594901980809315	29.6751881235	19.4767358193643
32	25.861282999948937	26.115859008341957	29.672031455270474	18.35082653643863
33	25.13348528667654	26.128299994893624	29.02287191818659	19.71534280024325
34	20.611279518329564	27.57061884623776	30.477538912899167	21.340562722533505
35	20.993422060468767	27.058588690771852	30.71995246429019	21.228036784469193
36	25.981607765403847	26.507750084719405	28.70284146562249	18.80780068425426
37	22.00383442346704	29.451714581486137	29.086655185059673	19.45779580998714
38	21.583626176207076	30.637693403956046	26.631138675220615	21.147541744616255
39	21.65632238866942	29.195374552611913	28.097503911019096	21.05079914769958
40	22.490518389913518	26.578310903967655	28.475282823546888	22.455887882571943
41	20.305546915981562	25.94177803980187	29.186554450205882	24.566120594010687
42	22.315601832724436	26.88896419502639	27.42829024635939	23.367143725889786
43	22.085443581518636	26.84040721020161	27.490216649568044	23.58393255871171
44	20.831596392113898	28.690307635887603	29.04218330029663	21.435912671701864
45	20.60895843874903	30.93989796534164	27.03194269718732	21.419200898722014
46	21.8785889693014	29.218771034783696	28.972365226514157	19.930274769400743
47	21.962983422849636	27.312607640065544	28.907560684625633	21.816848252459184
48	22.057126410636116	26.39986630581616	30.65041292005738	20.892594363490346
49	22.060375922048863	25.39233208149775	30.53343050919844	22.013861487254953
50	20.505159759907528	28.87033056815386	29.67221714163692	20.952292530301694
51	20.262281992600396	29.801269166314636	28.323577062163153	21.612871778921814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	958.0
1	1540.0
2	2122.0
3	13733.5
4	25345.0
5	17431.0
6	9517.0
7	9392.5
8	9268.0
9	9272.5
10	9277.0
11	9106.5
12	8936.0
13	8550.5
14	8165.0
15	7716.5
16	7268.0
17	7020.0
18	6772.0
19	6511.0
20	6250.0
21	6435.5
22	6621.0
23	7245.0
24	7869.0
25	8600.0
26	10512.0
27	11693.0
28	13800.5
29	15908.0
30	18502.0
31	21096.0
32	23822.0
33	26548.0
34	28714.0
35	30880.0
36	34176.5
37	37473.0
38	45917.0
39	54361.0
40	62093.5
41	69826.0
42	75169.5
43	80513.0
44	79844.0
45	79175.0
46	78932.5
47	78690.0
48	77203.5
49	75717.0
50	71436.0
51	67155.0
52	62548.5
53	57942.0
54	54696.5
55	51451.0
56	49172.0
57	46893.0
58	43923.0
59	40953.0
60	37621.0
61	34289.0
62	30111.5
63	25934.0
64	22091.0
65	18248.0
66	15361.0
67	12474.0
68	10384.0
69	8294.0
70	7327.0
71	6360.0
72	5169.5
73	3979.0
74	2915.5
75	1340.0
76	828.0
77	629.0
78	430.0
79	329.5
80	229.0
81	171.0
82	113.0
83	74.0
84	35.0
85	24.0
86	13.0
87	14.5
88	16.0
89	11.0
90	6.0
91	4.5
92	3.0
93	4.0
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1077085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.312928344759854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27371430941307	21.90827923070883
2	6.559957587448255	3.9770304858596535
3	3.2687757035459697	2.9725849103004283
4	2.2857989277074537	2.771570364244998
5	1.783338471954235	2.702910565740113
6	1.472312978025118	2.677807068236124
7	1.2353406846304358	2.621275555819828
8	1.0883436782513312	2.639270714664398
9	0.990880008277188	2.7032827220245306
>10	8.89333993188393	50.064268112722274
>50	0.12452336913448436	2.305897263550584
>100	0.02244451337940107	1.0649947183921726
>500	3.074590872817074E-4	0.049582321698727325
>1k	3.074590872817074E-4	0.0943182510509625
>5k	6.149181745634148E-4	1.4469277149863564
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9941	0.9229540844037378	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5584	0.5184363351081855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007891670573817294	0.0
2	0.0	0.0	0.0	0.02321079580534498	0.0
3	0.0	0.0	0.0	0.037044430105330595	0.0
4	0.0	0.0	0.0	0.05802698951336246	0.0
5	0.0	0.0	0.0	0.11475417446162559	0.0
6	0.0	0.0	0.0	0.20202676668972272	0.0
7	0.0	0.0	0.0	0.252440615178932	0.0
8	0.0	0.0	0.0	0.3409201687889071	0.0
9	0.0	0.0	0.0	0.47294317532970936	0.0
10	0.0	0.0	0.0	0.6511092439315375	0.0
11	0.0	0.0	0.0	0.838931003588389	0.0
12	0.0	0.0	0.0	0.9781029352372376	0.0
13	0.0	0.0	0.0	1.04151482937744	0.0
14	0.0	0.0	0.0	1.0702962161760678	0.0
15	0.0	0.0	0.0	1.1037197621357646	0.0
16	0.0	0.0	0.0	1.172238031353143	0.0
17	0.0	0.0	0.0	1.2531972871221864	0.0
18	0.0	0.0	0.0	1.3672087161180408	0.0
19	0.0	0.0	0.0	1.429785021609251	0.0
20	0.0	0.0	0.0	1.4973748589944156	0.0
21	0.0	0.0	0.0	1.5805623511607718	0.0
22	0.0	0.0	0.0	1.670991611618396	0.0
23	0.0	0.0	0.0	1.7724692108793643	0.0
24	0.0	0.0	0.0	1.8536141530148502	0.0
25	0.0	0.0	0.0	1.9283529155080612	0.0
26	0.0	0.0	0.0	1.9973354006415465	0.0
27	9.284318322137993E-5	0.0	0.0	2.0689174949052305	0.0
28	9.284318322137993E-5	0.0	0.0	2.149691064307831	0.0
29	9.284318322137993E-5	0.0	0.0	2.2378920883681417	0.0
30	9.284318322137993E-5	0.0	0.0	2.3404838058277666	0.0
31	9.284318322137993E-5	0.0	0.0	2.4428898369209486	0.0
32	9.284318322137993E-5	0.0	0.0	2.5349902746765576	0.0
33	9.284318322137993E-5	0.0	0.0	2.6336825784408844	0.0
34	9.284318322137993E-5	0.0	0.0	2.738131159564937	0.0
35	9.284318322137993E-5	0.0	0.0	2.8546493545077687	0.0
36	9.284318322137993E-5	0.0	0.0	2.9618832311284624	0.0
37	9.284318322137993E-5	0.0	0.0	3.0696741668484844	0.0
38	9.284318322137993E-5	0.0	0.0	3.1918557959678204	0.0
39	9.284318322137993E-5	0.0	0.0	3.341519007320685	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGG	20	7.03309E-4	45.0	1
CTATACG	35	1.211829E-7	45.0	1
CGCATCG	35	1.211829E-7	45.0	21
CGCGTAG	20	7.03309E-4	45.0	1
TCGAACA	20	7.03309E-4	45.0	41
TAGTACG	40	6.8139343E-9	45.0	1
TATAGCG	30	2.165374E-6	44.999996	1
CGTTTTT	8270	0.0	43.93894	1
GCGTAAG	90	0.0	42.5	1
TCACGAC	245	0.0	42.2449	25
TACGCGA	65	0.0	41.53846	2
TTACGAG	120	0.0	41.249996	3
CGTAAGA	105	0.0	40.714287	2
ACGACGG	250	0.0	40.500004	27
GTTTTTA	5930	0.0	40.408936	2
TAACGGG	90	0.0	40.0	3
TGGTTCG	45	1.9281288E-8	40.0	37
TATACGA	45	1.9281288E-8	40.0	2
ACGTAGA	85	0.0	39.705883	2
CGGTCTA	250	0.0	39.600002	31
>>END_MODULE