FastQCFastQC Report
Thu 26 May 2016
SRR1546926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546926_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences972573
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.3398202499966584No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18300.1881606830541255No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13170.13541399977173948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC351.2116652E-745.0000046
GTGTCGA351.2116652E-745.0000042
CCTTACG207.032752E-445.039
ACACGTA207.032752E-445.010
ACCGGCA207.032752E-445.031
CGAACAG253.8903614E-545.016
ACCGCGA207.032752E-445.02
TCGATAC453.8380676E-1045.031
TCTAGCG207.032752E-445.01
CCGTCTA253.8903614E-545.016
ATCCGAT207.032752E-445.041
GCGTAAG551.8189894E-1245.01
CACGATA302.1651558E-644.99999635
TTCGCGA650.044.9999962
CGACGGT1850.043.78378328
TCACGAC1800.043.7525
AACGCCG1200.043.12499613
ATTGCGA1200.043.1249962
CGTAAGA1050.042.8571432
CGTTTTT30250.042.8429761