##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546926_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	972573
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.799781610223604	31.0	31.0	34.0	30.0	34.0
2	32.1385983365773	33.0	31.0	34.0	30.0	34.0
3	32.16809123839547	33.0	31.0	34.0	30.0	34.0
4	35.88557465609265	37.0	35.0	37.0	35.0	37.0
5	35.87743953410181	37.0	35.0	37.0	35.0	37.0
6	35.92443343584492	37.0	35.0	37.0	35.0	37.0
7	36.155491670034024	37.0	35.0	37.0	35.0	37.0
8	13.291176086525125	0.0	0.0	37.0	0.0	37.0
9	25.034321331149435	18.0	17.0	39.0	17.0	39.0
10	32.793298806362095	32.0	32.0	38.0	27.0	39.0
11	35.67185496615678	37.0	35.0	37.0	32.0	39.0
12	36.61620772939409	37.0	35.0	39.0	33.0	39.0
13	36.736086648508646	39.0	35.0	39.0	33.0	39.0
14	37.90658696056749	40.0	37.0	41.0	33.0	41.0
15	38.26882506505938	40.0	37.0	41.0	33.0	41.0
16	38.409781065277365	40.0	37.0	41.0	34.0	41.0
17	38.40692369621612	40.0	37.0	41.0	34.0	41.0
18	38.375416549708866	40.0	37.0	41.0	34.0	41.0
19	38.32233364487807	40.0	37.0	41.0	34.0	41.0
20	38.289009668168866	40.0	37.0	41.0	34.0	41.0
21	38.198923885405	40.0	36.0	41.0	34.0	41.0
22	38.14535258535863	40.0	36.0	41.0	34.0	41.0
23	38.075213891399414	40.0	36.0	41.0	34.0	41.0
24	37.99232139901066	40.0	36.0	41.0	34.0	41.0
25	37.879975076421	40.0	35.0	41.0	33.0	41.0
26	37.80332581718802	40.0	35.0	41.0	33.0	41.0
27	37.73950541501769	40.0	35.0	41.0	33.0	41.0
28	37.72375029946338	40.0	35.0	41.0	33.0	41.0
29	37.7668185318737	40.0	36.0	41.0	33.0	41.0
30	37.69935213089403	40.0	36.0	41.0	33.0	41.0
31	37.59581542979293	40.0	35.0	41.0	33.0	41.0
32	37.44282537146312	40.0	35.0	41.0	33.0	41.0
33	37.325959079678334	40.0	35.0	41.0	33.0	41.0
34	37.20845633181263	40.0	35.0	41.0	33.0	41.0
35	37.094382632460494	40.0	35.0	41.0	32.0	41.0
36	36.977097863091	40.0	35.0	41.0	32.0	41.0
37	36.88042028721752	40.0	35.0	41.0	31.0	41.0
38	36.81653408021814	40.0	35.0	41.0	31.0	41.0
39	36.78317206009215	40.0	35.0	41.0	31.0	41.0
40	36.66096529515008	39.0	35.0	41.0	31.0	41.0
41	36.639637333135916	39.0	35.0	41.0	31.0	41.0
42	36.601057195706645	39.0	35.0	41.0	31.0	41.0
43	36.50765957928094	39.0	35.0	41.0	31.0	41.0
44	36.327263866054274	39.0	35.0	41.0	30.0	41.0
45	36.19824938590728	39.0	35.0	40.0	30.0	41.0
46	36.203787273551704	39.0	35.0	40.0	30.0	41.0
47	36.17310268740753	39.0	35.0	40.0	30.0	41.0
48	36.131976725654525	39.0	35.0	40.0	30.0	41.0
49	36.04308365541713	39.0	35.0	40.0	30.0	41.0
50	35.93311658867766	38.0	35.0	40.0	30.0	41.0
51	35.53594228916493	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	8.0
16	26.0
17	72.0
18	186.0
19	409.0
20	791.0
21	1464.0
22	2270.0
23	3656.0
24	5445.0
25	7859.0
26	10275.0
27	11560.0
28	12108.0
29	13650.0
30	17016.0
31	22562.0
32	30140.0
33	42832.0
34	68669.0
35	114273.0
36	98692.0
37	170985.0
38	245783.0
39	91810.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.13468500564996	23.555455477378047	29.878785448495897	13.431074068476093
2	31.391371136151218	24.86188697403691	31.5251400151968	12.221601874615068
3	28.708590511971856	25.02855826760562	33.304338080534826	12.958513139887701
4	24.916073137954683	27.316407097462093	32.97809007652896	14.789429688054264
5	23.46538511762099	31.771188383802553	30.437303935025955	14.3261225635505
6	19.946163424236534	41.71306421214654	27.861147697910592	10.479624665706327
7	87.96604470821214	4.288418452907905	6.179793187760714	1.5657436511192477
8	33.72836794770161	63.41385171087415	2.1197380556523777	0.7380422857718649
9	87.21741195776563	3.684659146408547	6.798461400840862	2.299467494984952
10	51.45156199071946	22.78636153789998	13.344705230352888	12.417371241027665
11	44.600662366732365	23.05451621626346	18.903465343989602	13.441356073014571
12	39.515491382137895	22.52602118298575	23.219439569060626	14.739047865815728
13	21.23192809177306	39.589418994769545	24.179264692727433	14.999388220729962
14	15.237519445841082	41.973815847242314	27.85590387559597	14.932760831320632
15	14.095702841843236	25.371771579099978	45.5677877136215	14.964737865435293
16	16.572329275026142	19.552876750639797	44.91251556438437	18.96227840994969
17	17.117481155656183	19.95983849027271	27.969520025746142	34.95316032832497
18	21.79044657830312	24.463253658080163	32.79075195383791	20.955547809778803
19	28.37905226651367	25.689896799520444	26.091306256702584	19.8397446772633
20	30.680781802497087	24.259464328127557	24.725341953765938	20.33441191560942
21	23.149110658017445	28.187190061825696	27.845621871057496	20.818077409099367
22	23.30344354613998	23.902987230778564	26.593170898225633	26.200398324855822
23	20.117358799802172	29.746044769904163	25.82736720019988	24.30922923009378
24	19.850849242164855	24.733361917305952	37.54196343102266	17.873825409506537
25	17.8139841430926	26.290263044522106	34.91007872930875	20.985674083076542
26	17.072445975777654	34.014104853825884	27.31938887877825	21.594060291618213
27	18.276777167369442	33.41846833091192	30.065403830869254	18.239350670849387
28	15.412313522995188	28.582224676193974	39.040462772460266	16.964999028350572
29	15.44274825642908	26.46546840185775	37.672339248570545	20.419444093142623
30	19.683663848369225	29.114626871196304	32.7287514664709	18.47295781396358
31	29.82562748503197	26.2943758463375	24.546332254751057	19.33366441387947
32	28.912791122106	27.271886017810488	26.743699444668934	17.071623415414575
33	26.48613523098009	27.494799876204667	25.584609073046444	20.4344558197688
34	20.15715015736608	27.864232299272135	28.14996920539641	23.828648337965376
35	19.768696025902425	26.039999054055585	31.087023801812308	23.104281118229686
36	30.22004517912794	24.69932848228359	26.534460652310933	18.546165686277533
37	21.195838255843004	31.087435081993846	29.060646347369296	18.65608031479385
38	20.24084567430928	32.82920665081181	24.54417303379798	22.38577464108093
39	20.44720550539651	29.877757248042048	28.076144412810145	21.598892833751297
40	22.986140886082588	25.706450826827393	26.838807986649844	24.468600300440173
41	18.00461250723596	24.499549134100988	28.541713578312372	28.954124780350675
42	22.085642928602788	26.401308693537658	24.550445056566446	26.962603321293106
43	22.0889331700551	26.922297863502276	26.001030256854758	24.987738709587866
44	20.608221696469055	30.46917814909523	28.462542143366104	20.460058011069606
45	18.581330141799125	36.24334625781304	23.6212603064243	21.554063293963537
46	23.12731280839587	31.189638207106306	26.16903821101347	19.514010773484355
47	22.078137065289702	28.361161578616723	27.06408670608787	22.496614650005707
48	23.081249428063497	25.250649565636717	30.060982568917705	21.607118437382077
49	20.722968867118457	25.68629809793198	30.811466080181127	22.779266954768435
50	19.91223280925956	31.166298056803964	28.125909314776372	20.795559819160104
51	18.876115211917256	32.85254680111416	25.604042061624167	22.667295925344423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	902.0
1	1278.5
2	1655.0
3	5112.5
4	8570.0
5	6164.5
6	3759.0
7	4076.0
8	4393.0
9	4675.5
10	4958.0
11	4823.5
12	4689.0
13	4555.0
14	4421.0
15	4305.0
16	4189.0
17	3858.5
18	3528.0
19	3569.0
20	3610.0
21	3786.0
22	3962.0
23	4443.0
24	4924.0
25	5765.0
26	8573.5
27	10541.0
28	12392.0
29	14243.0
30	15925.0
31	17607.0
32	19716.0
33	21825.0
34	24401.5
35	26978.0
36	31161.0
37	35344.0
38	47530.0
39	59716.0
40	71882.0
41	84048.0
42	88181.5
43	92315.0
44	90383.0
45	88451.0
46	86706.0
47	84961.0
48	81025.0
49	77089.0
50	72184.0
51	67279.0
52	60360.5
53	53442.0
54	48731.0
55	44020.0
56	40706.5
57	37393.0
58	33875.0
59	30357.0
60	25847.5
61	21338.0
62	18283.5
63	15229.0
64	13198.0
65	11167.0
66	9179.0
67	7191.0
68	5959.5
69	4728.0
70	3975.0
71	3222.0
72	2644.5
73	2067.0
74	1531.0
75	711.0
76	427.0
77	324.0
78	221.0
79	184.5
80	148.0
81	93.0
82	38.0
83	29.5
84	21.0
85	12.5
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	972573.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.369527748571425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84375983576308	16.96589090202089
2	5.658152465422438	2.5314039716183006
3	2.2596903713197216	1.5164461939324854
4	1.421707518714074	1.2721170316090842
5	1.05608656108418	1.1812078816532967
6	0.8446737440874178	1.1336971654111927
7	0.7505526921515631	1.1752656491694562
8	0.7005784774878673	1.2537287753773414
9	0.5852251232555666	1.178208867044388
>10	9.624787318883246	51.261470370356996
>50	1.1655874840591245	16.72621005016176
>100	0.08642540027626647	2.9267197425627134
>500	0.0013865037477475903	0.210594797258831
>1k	0.0013865037477475903	0.6670386018232585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3305	0.3398202499966584	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1830	0.1881606830541255	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1317	0.13541399977173948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008431243721550978	0.0
2	0.0	0.0	0.0	0.03413625506774299	0.0
3	0.0	0.0	0.0	0.05295232337315554	0.0
4	0.0	0.0	0.0	0.08143347594473628	0.0
5	0.0	0.0	0.0	0.1408634621771322	0.0
6	0.0	0.0	0.0	0.20378932995261023	0.0
7	0.0	0.0	0.0	0.24615118865113467	0.0
8	0.0	0.0	0.0	0.3409512704958908	0.0
9	0.0	0.0	0.0	0.43595699243141645	0.0
10	0.0	0.0	0.0	0.6371758212494075	0.0
11	0.0	0.0	0.0	0.8737647456797587	0.0
12	0.0	0.0	0.0	1.094416563075471	0.0
13	0.0	0.0	0.0	1.177289519655594	0.0
14	0.0	0.0	0.0	1.2132765355402628	0.0
15	0.0	0.0	0.0	1.2567694147380197	0.0
16	0.0	0.0	0.0	1.3690489042981864	0.0
17	0.0	0.0	0.0	1.4921244986237536	0.0
18	0.0	0.0	0.0	1.6607493730547733	0.0
19	0.0	0.0	0.0	1.7476323114049022	0.0
20	0.0	0.0	0.0	1.8330757691196444	0.0
21	0.0	0.0	0.0	1.9586190445344462	0.0
22	0.0	0.0	0.0	2.0847792402215566	0.0
23	0.0	0.0	0.0	2.234382406256394	0.0
24	0.0	0.0	0.0	2.3369968115503927	0.0
25	0.0	0.0	0.0	2.428506651942836	0.0
26	0.0	0.0	0.0	2.5220728932429752	0.0
27	0.0	0.0	0.0	2.6060768703223305	0.0
28	0.0	0.0	0.0	2.701699512530165	0.0
29	0.0	0.0	0.0	2.8028744371887764	0.0
30	0.0	0.0	0.0	2.921014669335875	0.0
31	0.0	0.0	0.0	3.0439874436160577	0.0
32	0.0	0.0	0.0	3.15143439104314	0.0
33	0.0	0.0	0.0	3.261451839604842	0.0
34	0.0	0.0	0.0	3.3826766731134836	0.0
35	0.0	0.0	0.0	3.5389631420983307	0.0
36	0.0	0.0	0.0	3.6622443765146677	0.0
37	0.0	0.0	0.0	3.7793564082079185	0.0
38	0.0	0.0	0.0	3.905825064031183	0.0
39	0.0	0.0	0.0	4.0394911230313815	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	35	1.2116652E-7	45.000004	6
GTGTCGA	35	1.2116652E-7	45.000004	2
CCTTACG	20	7.032752E-4	45.0	39
ACACGTA	20	7.032752E-4	45.0	10
ACCGGCA	20	7.032752E-4	45.0	31
CGAACAG	25	3.8903614E-5	45.0	16
ACCGCGA	20	7.032752E-4	45.0	2
TCGATAC	45	3.8380676E-10	45.0	31
TCTAGCG	20	7.032752E-4	45.0	1
CCGTCTA	25	3.8903614E-5	45.0	16
ATCCGAT	20	7.032752E-4	45.0	41
GCGTAAG	55	1.8189894E-12	45.0	1
CACGATA	30	2.1651558E-6	44.999996	35
TTCGCGA	65	0.0	44.999996	2
CGACGGT	185	0.0	43.783783	28
TCACGAC	180	0.0	43.75	25
AACGCCG	120	0.0	43.124996	13
ATTGCGA	120	0.0	43.124996	2
CGTAAGA	105	0.0	42.857143	2
CGTTTTT	3025	0.0	42.842976	1
>>END_MODULE