##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546925_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	813568
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69829073906545	31.0	31.0	33.0	30.0	34.0
2	32.066117398914415	33.0	31.0	34.0	30.0	34.0
3	32.10627261642542	33.0	31.0	34.0	30.0	34.0
4	35.855295439348644	37.0	35.0	37.0	35.0	37.0
5	35.83481036618943	37.0	35.0	37.0	35.0	37.0
6	35.87043246538703	37.0	35.0	37.0	35.0	37.0
7	36.11125191747955	37.0	35.0	37.0	35.0	37.0
8	13.265561084015104	0.0	0.0	36.0	0.0	37.0
9	25.00333223528949	18.0	17.0	39.0	17.0	39.0
10	32.47724713853052	32.0	32.0	37.0	27.0	39.0
11	35.466383879405285	37.0	34.0	37.0	32.0	39.0
12	36.50488588538389	37.0	35.0	39.0	33.0	39.0
13	36.66743160989616	39.0	35.0	39.0	33.0	39.0
14	37.826533246145374	40.0	36.0	41.0	33.0	41.0
15	38.174477118077405	40.0	37.0	41.0	33.0	41.0
16	38.30310926683449	40.0	37.0	41.0	34.0	41.0
17	38.30048010737885	40.0	37.0	41.0	34.0	41.0
18	38.23565577800503	40.0	37.0	41.0	34.0	41.0
19	38.225949152375705	40.0	37.0	41.0	34.0	41.0
20	38.195060523521086	40.0	36.0	41.0	34.0	41.0
21	38.11233111430145	40.0	36.0	41.0	34.0	41.0
22	38.061294200361864	40.0	36.0	41.0	33.0	41.0
23	37.98969477658905	40.0	36.0	41.0	34.0	41.0
24	37.91148373584015	40.0	36.0	41.0	34.0	41.0
25	37.80559707363121	40.0	35.0	41.0	33.0	41.0
26	37.73313109660164	40.0	35.0	41.0	33.0	41.0
27	37.67612172553493	40.0	35.0	41.0	33.0	41.0
28	37.65068808016048	40.0	35.0	41.0	33.0	41.0
29	37.68737216803021	40.0	36.0	41.0	33.0	41.0
30	37.60785453705161	40.0	36.0	41.0	33.0	41.0
31	37.53687583582442	40.0	35.0	41.0	33.0	41.0
32	37.393132473253615	40.0	35.0	41.0	33.0	41.0
33	37.26984714246382	40.0	35.0	41.0	32.0	41.0
34	37.13228396397105	40.0	35.0	41.0	32.0	41.0
35	37.01124921334173	40.0	35.0	41.0	32.0	41.0
36	36.91642001651982	40.0	35.0	41.0	31.0	41.0
37	36.84887188286658	40.0	35.0	41.0	31.0	41.0
38	36.780372384361236	40.0	35.0	41.0	31.0	41.0
39	36.73346911382945	40.0	35.0	41.0	31.0	41.0
40	36.619615078272496	39.0	35.0	41.0	31.0	41.0
41	36.60681836060415	39.0	35.0	41.0	31.0	41.0
42	36.56876622482694	39.0	35.0	41.0	31.0	41.0
43	36.4787958720107	39.0	35.0	41.0	30.0	41.0
44	36.30221198473883	39.0	35.0	41.0	30.0	41.0
45	36.206330632473254	39.0	35.0	40.0	30.0	41.0
46	36.2142279440686	39.0	35.0	40.0	30.0	41.0
47	36.18303817259282	39.0	35.0	40.0	30.0	41.0
48	36.12923812146004	39.0	35.0	40.0	30.0	41.0
49	36.06409298300818	39.0	35.0	40.0	30.0	41.0
50	35.94805597073631	38.0	35.0	40.0	29.0	41.0
51	35.56734901077722	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	5.0
15	17.0
16	22.0
17	73.0
18	211.0
19	381.0
20	718.0
21	1256.0
22	2079.0
23	3234.0
24	4711.0
25	7161.0
26	8983.0
27	9980.0
28	10505.0
29	12116.0
30	14896.0
31	19667.0
32	26486.0
33	36563.0
34	57653.0
35	91513.0
36	83277.0
37	146561.0
38	197546.0
39	77933.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.88067868942731	23.649528988357456	30.445641913152926	14.024150409062303
2	30.97380919603524	25.14381096601636	31.422081497797354	12.460298340151038
3	28.80988436123348	25.077436674008812	32.99761052548773	13.115068439269981
4	25.48969477658905	27.78464738042794	32.23197077564506	14.493687067337948
5	23.234566747954688	32.13499056010069	30.477231159534295	14.153211532410321
6	20.29160438955318	41.277680538074264	27.995693045940843	10.435022026431719
7	88.66253343297672	3.9093228838892387	6.061079098489616	1.3670645846444305
8	33.91566531623663	63.40920488514789	2.000201581183134	0.6749282174323473
9	87.67048359817495	3.1749036343612334	6.905015929830081	2.249596837633732
10	48.65690390969163	19.639784258967904	19.368755899937067	12.334555931403399
11	44.90097938955318	20.937278752359976	18.724925267463814	15.436816590623033
12	40.130511524543735	22.633018997797357	21.63359424166142	15.602875235997482
13	23.377763137193202	36.26666732221523	23.951040355569543	16.404529185022028
14	18.115142188483325	38.89840800031467	26.6952485840151	16.29120122718691
15	16.797612492133418	24.49911992605412	43.015826581183134	15.687441000629326
16	18.606926526117054	19.295129601950915	42.803797592825674	19.294146279106357
17	19.169878854625548	19.719556324732537	27.92132925582127	33.18923556482064
18	23.128982457520454	24.90080730805538	31.63583130113279	20.33437893329138
19	28.299048143486473	26.529804515418505	25.044987020138453	20.126160320956576
20	30.522832756450597	25.057647301762113	23.690951463184394	20.728568478602895
21	24.45708287444934	27.096690135305224	28.04977580239144	20.396451187853994
22	22.866681088735053	23.330071979232223	27.47883397577093	26.3244129562618
23	20.662562932662052	29.087181403398365	26.38611646475771	23.864139199181874
24	21.462127320641912	25.288236508810574	35.013299441472626	18.23633672907489
25	20.214536461611075	25.02937676998112	33.417858126179986	21.338228642227815
26	18.742379247954688	32.69216586689742	26.34026903713027	22.22518584801762
27	18.916427391441157	31.54905306010069	30.036456694461926	19.498062853996224
28	16.54883181246067	27.7828036500944	37.63164234581498	18.036722191629956
29	17.20642896475771	26.600112098804278	35.30092137350535	20.892537562932663
30	19.655886170547515	28.303350180931403	32.027931285399625	20.01283236312146
31	29.279421019509126	25.56381273599748	25.180685572687224	19.97608067180617
32	28.98307209723096	27.37189761642542	25.680459408432977	17.964570877910635
33	26.357231356198866	26.80329118156073	25.61322470893644	21.226252753303964
34	19.938714403713025	27.947756057268723	28.192111784140973	23.92141775487728
35	20.321964482378853	25.70639454845815	30.61219221994965	23.359448749213342
36	28.363332874449338	25.233785006293267	27.05772596758968	19.345156151667716
37	21.216296609502834	30.37324476872247	28.44912779263688	19.96133082913782
38	21.30159986626809	32.139415512901195	24.538452839836374	22.020531780994336
39	21.10419780522341	29.208007197923223	26.601095421648836	23.08669957520453
40	23.231616779421017	26.10144450125865	26.65591567023285	24.011023049087477
41	18.742379247954688	24.39525645059786	28.540453901825046	28.321910399622404
42	22.690297553492762	26.578478996224042	24.455362059471366	26.275861390811833
43	22.539726242920075	26.498952761170546	25.052484856828194	25.90883613908118
44	20.88196684235368	29.956438797986156	27.738799952800502	21.422794406859662
45	19.949899701069857	33.96876474984267	23.324172042164886	22.75716350692259
46	23.36030915670233	30.45559805695406	26.037282685651352	20.146810100692257
47	22.892616228760225	27.549387389867842	26.37468533668974	23.18331104468219
48	22.69140379169289	25.418403870358713	29.4708002281309	22.419392109817498
49	21.559107536186282	24.824722702957835	30.304658000314667	23.31151176054122
50	20.05634439899308	30.45338558055381	28.019292794210195	21.470977226242923
51	19.96944324260541	31.924559471365637	25.641618156073005	22.464379129955947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	456.0
1	735.5
2	1015.0
3	3658.5
4	6302.0
5	4636.0
6	2970.0
7	3072.5
8	3175.0
9	3376.5
10	3578.0
11	3582.0
12	3586.0
13	3375.5
14	3165.0
15	3029.5
16	2894.0
17	2786.0
18	2678.0
19	2570.0
20	2462.0
21	2465.5
22	2469.0
23	2663.5
24	2858.0
25	3399.5
26	4745.5
27	5550.0
28	7036.5
29	8523.0
30	10621.0
31	12719.0
32	14405.5
33	16092.0
34	17762.0
35	19432.0
36	22674.0
37	25916.0
38	35171.5
39	44427.0
40	55331.0
41	66235.0
42	70748.0
43	75261.0
44	75506.0
45	75751.0
46	76313.0
47	76875.0
48	73071.0
49	69267.0
50	65183.0
51	61099.0
52	56214.0
53	51329.0
54	47250.0
55	43171.0
56	39566.5
57	35962.0
58	32535.0
59	29108.0
60	24646.0
61	20184.0
62	16949.0
63	13714.0
64	11126.0
65	8538.0
66	6748.5
67	4959.0
68	4125.5
69	3292.0
70	2712.5
71	2133.0
72	1700.5
73	1268.0
74	946.0
75	453.5
76	283.0
77	211.5
78	140.0
79	111.0
80	82.0
81	61.5
82	41.0
83	38.0
84	35.0
85	20.0
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	813568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.889246307623942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.41809739484286	18.489607142769625
2	6.167911264100639	3.193651478397392
3	3.11101606063282	2.4162558318209104
4	2.0916673791479807	2.1660676788953728
5	1.6716440291465864	2.1638802004622435
6	1.4326746545246452	2.2254520205807147
7	1.2114063520314051	2.195367819845287
8	1.0962235979599526	2.270432218865195
9	0.9589803734539681	2.2344551183274275
>10	10.5065959169288	55.589135791837904
>50	0.29706684974475484	4.719062921019327
>100	0.033855130538607905	1.5610053266284407
>500	0.001430498473462306	0.21751612380540233
>1k	0.001430498473462306	0.5581103267447355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2218	0.272626258653241	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1202	0.1477442573945878	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1101	0.1353298064820642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007252005978602895	0.0
2	0.0	0.0	0.0	0.02913093926998112	0.0
3	0.0	0.0	0.0	0.04609325833857772	0.0
4	0.0	0.0	0.0	0.07608460509754562	0.0
5	0.0	0.0	0.0	0.14381096601636248	0.0
6	0.0	0.0	0.0	0.22702466173694147	0.0
7	0.0	0.0	0.0	0.27483873505349277	0.0
8	0.0	0.0	0.0	0.3764897341095028	0.0
9	0.0	0.0	0.0	0.47887822529893015	0.0
10	0.0	0.0	0.0	0.6786156780994336	0.0
11	0.0	0.0	0.0	0.8768781466331026	0.0
12	0.0	0.0	0.0	1.0417076384518564	0.0
13	0.0	0.0	0.0	1.117300582127124	0.0
14	0.0	0.0	0.0	1.1481523363750787	0.0
15	0.0	0.0	0.0	1.191172710824418	0.0
16	0.0	0.0	0.0	1.277582205789805	0.0
17	0.0	0.0	0.0	1.3797248662680932	0.0
18	0.0	0.0	0.0	1.5299274307740718	0.0
19	0.0	0.0	0.0	1.595932976714915	0.0
20	0.0	0.0	0.0	1.6728779893014474	0.0
21	0.0	0.0	0.0	1.7869434392699812	0.0
22	0.0	0.0	0.0	1.9024838735053493	0.0
23	0.0	0.0	0.0	2.0228180066079293	0.0
24	0.0	0.0	0.0	2.1217648678414096	0.0
25	0.0	0.0	0.0	2.2094035163624923	0.0
26	0.0	0.0	0.0	2.3002379641283826	0.0
27	0.0	0.0	0.0	2.3881224433606043	0.0
28	0.0	0.0	0.0	2.4787110604153555	0.0
29	0.0	0.0	0.0	2.574339207048458	0.0
30	0.0	0.0	0.0	2.6940587633731905	0.0
31	0.0	0.0	0.0	2.809845028319698	0.0
32	0.0	0.0	0.0	2.9132168423536817	0.0
33	0.0	0.0	0.0	3.0218740166771556	0.0
34	0.0	0.0	0.0	3.127089561044682	0.0
35	0.0	0.0	0.0	3.2720067652611706	0.0
36	0.0	0.0	0.0	3.3993470736312146	0.0
37	0.0	0.0	0.0	3.5222624292007554	0.0
38	0.0	0.0	0.0	3.6494798222152296	0.0
39	0.0	0.0	0.0	3.800911540276904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAA	30	2.1647156E-6	45.000004	32
ATTACGA	30	2.1647156E-6	45.000004	2
TTCGTCG	20	7.032069E-4	45.000004	1
CGCGAGT	70	0.0	45.000004	4
TAGCCGT	20	7.032069E-4	45.000004	44
ATATACG	20	7.032069E-4	45.000004	1
TCTAACG	20	7.032069E-4	45.000004	1
GCGATAC	35	1.2113378E-7	45.000004	9
CGCACCG	20	7.032069E-4	45.000004	44
GCGTAAG	35	1.2113378E-7	45.000004	1
TCGCGGA	30	2.1647156E-6	45.000004	2
ATCGATC	20	7.032069E-4	45.000004	38
AATCGCG	20	7.032069E-4	45.000004	1
TAGTACG	40	6.8102963E-9	45.000004	1
CGACGGT	200	0.0	45.0	28
TAACGCC	85	0.0	45.0	12
CGTTAGA	55	1.8189894E-12	45.0	2
CTACGCG	25	3.8897968E-5	45.0	1
TATCGTG	50	2.1827873E-11	45.0	1
CACGACG	205	0.0	43.90244	26
>>END_MODULE