FastQCFastQC Report
Thu 26 May 2016
SRR1546924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546924_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1043926
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.24743133133957768No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18620.1783651331607796No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13820.13238486252857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10520.10077342646892595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA207.0329884E-445.00000426
GATACGA453.8562575E-1045.0000042
GGTACGC207.0329884E-445.00000445
GGTACGA207.0329884E-445.0000042
CGTATTT207.0329884E-445.00000423
TTAATCG406.8139343E-945.00000410
ACGTTGT207.0329884E-445.00000420
ACCGACA207.0329884E-445.00000427
CGACATA207.0329884E-445.00000429
AAGCGAT207.0329884E-445.00000445
CGTTCCC207.0329884E-445.00000419
TCGGCCA207.0329884E-445.00000413
GTTACAC207.0329884E-445.00000433
TTCCCGC207.0329884E-445.00000429
GCCGAAC207.0329884E-445.00000437
GTTAACG207.0329884E-445.00000427
TACGAAC207.0329884E-445.00000434
TCGTATT207.0329884E-445.00000422
AACTCCG302.1653086E-645.00000443
CGCACGA302.1653086E-645.00000439