##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546924_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1043926
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84236909512743	31.0	31.0	34.0	30.0	34.0
2	32.18346990112326	33.0	31.0	34.0	30.0	34.0
3	32.19869894992557	33.0	31.0	34.0	30.0	34.0
4	35.917596649570946	37.0	35.0	37.0	35.0	37.0
5	35.89671490124779	37.0	35.0	37.0	35.0	37.0
6	35.94005801177478	37.0	35.0	37.0	35.0	37.0
7	36.16462277977558	37.0	35.0	37.0	35.0	37.0
8	13.13061653795384	0.0	0.0	37.0	0.0	37.0
9	24.931197230455034	18.0	17.0	39.0	17.0	39.0
10	32.899402831235165	32.0	32.0	38.0	27.0	39.0
11	35.76051655002366	37.0	35.0	37.0	32.0	39.0
12	36.54324061284037	37.0	35.0	39.0	33.0	39.0
13	36.50497640637363	39.0	35.0	39.0	33.0	39.0
14	37.66856175629307	39.0	36.0	41.0	33.0	41.0
15	38.08609230922498	40.0	36.0	41.0	33.0	41.0
16	38.26095623636158	40.0	36.0	41.0	34.0	41.0
17	38.28083599795388	40.0	36.0	41.0	34.0	41.0
18	38.24396556844067	40.0	36.0	41.0	34.0	41.0
19	38.190152367121804	40.0	36.0	41.0	34.0	41.0
20	38.146534332893324	40.0	36.0	41.0	34.0	41.0
21	38.03545653619126	40.0	36.0	41.0	34.0	41.0
22	37.98939196839623	40.0	36.0	41.0	34.0	41.0
23	37.909595124558635	40.0	35.0	41.0	34.0	41.0
24	37.835473012454905	40.0	35.0	41.0	34.0	41.0
25	37.7184675925305	40.0	35.0	41.0	33.0	41.0
26	37.629499600546396	40.0	35.0	41.0	33.0	41.0
27	37.55145383868205	40.0	35.0	41.0	33.0	41.0
28	37.56222088538843	40.0	35.0	41.0	33.0	41.0
29	37.6353611271297	40.0	35.0	41.0	33.0	41.0
30	37.55957893567169	40.0	35.0	41.0	33.0	41.0
31	37.42791155694944	40.0	35.0	41.0	33.0	41.0
32	37.25231098756042	40.0	35.0	41.0	33.0	41.0
33	37.12038018020434	40.0	35.0	41.0	33.0	41.0
34	37.01459777800342	40.0	35.0	41.0	32.0	41.0
35	36.909840352668674	40.0	35.0	41.0	32.0	41.0
36	36.77713937577951	40.0	35.0	41.0	31.0	41.0
37	36.65729467414357	39.0	35.0	41.0	31.0	41.0
38	36.61074060805076	39.0	35.0	41.0	31.0	41.0
39	36.55707301092223	39.0	35.0	41.0	31.0	41.0
40	36.419455018842335	39.0	35.0	41.0	30.0	41.0
41	36.404812218490584	39.0	35.0	41.0	31.0	41.0
42	36.3677272143811	39.0	35.0	41.0	31.0	41.0
43	36.25743970358052	39.0	35.0	41.0	30.0	41.0
44	36.05691782750885	39.0	35.0	40.0	30.0	41.0
45	35.95402643482392	38.0	35.0	40.0	30.0	41.0
46	35.95381760776147	38.0	35.0	40.0	30.0	41.0
47	35.93163883263756	38.0	35.0	40.0	30.0	41.0
48	35.91844536873303	38.0	35.0	40.0	30.0	41.0
49	35.85704925444907	38.0	35.0	40.0	29.0	41.0
50	35.70931177114087	38.0	35.0	40.0	29.0	41.0
51	35.25472974138014	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	0.0
13	1.0
14	3.0
15	17.0
16	40.0
17	122.0
18	273.0
19	543.0
20	989.0
21	1636.0
22	2698.0
23	4284.0
24	6265.0
25	9213.0
26	11627.0
27	13004.0
28	13458.0
29	15337.0
30	18958.0
31	24862.0
32	33394.0
33	47796.0
34	80358.0
35	135094.0
36	102274.0
37	178126.0
38	260974.0
39	82551.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.60796263336673	22.694712077292838	30.359623191682168	14.337702097658264
2	30.25702971283405	26.258949389132948	30.823736548376036	12.660284349656967
3	28.73948919751017	25.326699402064897	33.099280983517986	12.834530416906945
4	25.680172732550012	28.000356347097398	31.51880497276627	14.800665947586323
5	24.08245412031121	31.148472209716015	30.486164728151227	14.282908941821546
6	21.628544552008474	40.52030507909564	27.18162015315262	10.669530215743261
7	87.69031521391362	4.329713025635916	6.295561179623843	1.68441058082661
8	32.981456540022954	64.00463251226618	2.1119313054756756	0.9019796422351776
9	86.08158049516919	4.571971576529371	6.907194571262713	2.439253357038717
10	58.46084875747898	21.254763268660806	11.088717016340238	9.195670957519978
11	53.07368529953273	18.664732940840633	16.940472792132777	11.321108967493865
12	46.6098171709489	21.3415510294791	19.873726681776294	12.174905117795706
13	20.621959794085022	46.45942336908938	20.12288227326458	12.795734563561018
14	13.555845912449731	46.81433358303175	26.51069137084429	13.119129133674226
15	12.348001678279878	23.11897586610545	51.94573178558633	12.587290670028336
16	13.974553751894291	17.75087506202547	49.82604130944147	18.448529876638766
17	14.7264269689614	16.970934721426616	27.3346003452352	40.968037964376784
18	22.419022037960545	22.37064696156624	34.29304375980673	20.917287240666486
19	28.890170376061135	24.71899349187586	24.899561846337768	21.491274285725233
20	33.49308284303677	22.692892024913643	23.171661592871526	20.642363539178064
21	22.259719558666035	27.84095807557241	27.961081532599053	21.938240833162503
22	22.752474792274548	24.136768314995507	25.045836582286483	28.06492031044346
23	18.804972766268875	30.138055762573206	23.972867808637776	27.084103662520143
24	20.433536476723447	23.120604334023675	39.90761797292145	16.538241216331425
25	15.603309046809832	25.01163875600378	37.313564371420966	22.07148782576543
26	15.481365537403994	37.22486076599299	26.72861869519487	20.565155001408144
27	16.35949291424871	35.67408034669124	29.42210463193751	18.544322107122536
28	13.093265231443606	29.039510463385337	41.29066619664612	16.57655810852493
29	13.861135751001507	24.663242413734306	39.615355877715466	21.860265957548716
30	17.974837296896524	29.930952960267298	32.99026942522746	19.10394031760872
31	30.862053440569543	26.456856137312414	23.75771845897123	18.923371963146813
32	30.828813536591674	27.5547308908869	26.411642204524078	15.20481336799735
33	28.034841550071555	28.76717315211998	24.49129536001594	18.706689937792525
34	19.777742866831556	29.20484785320032	27.531357586648863	23.486051693319258
35	19.549278397127765	26.786572994637552	30.68531677532699	22.978831832907694
36	32.234085557788575	23.498313098821182	26.939553186720133	17.32804815667011
37	20.457867703266324	31.905326622768282	29.598266543797163	18.03853913016823
38	20.723212181706366	32.71237616459404	23.112461994432557	23.451949659267036
39	20.234959182930588	31.591128106781515	27.74142994809977	20.432482762188126
40	24.112916049605047	25.691572007977577	25.540411868274187	24.655100074143185
41	17.288102796558377	23.424936250270616	28.457572663196434	30.829388289974574
42	23.746127599082694	24.966807992137376	24.298561392282593	26.98850301649734
43	23.514789362464388	26.094378337161828	25.327369947678285	25.0634623526955
44	19.355394922628616	32.48898868310589	27.5358598214816	20.619756572783896
45	17.3847571571165	39.84429930857168	20.7855729237513	21.985370610560516
46	23.961564325440694	31.91394792351182	25.270660947231892	18.853826803815597
47	22.62775330818468	28.577025574609692	26.30914451790644	22.486076599299185
48	24.60749133559275	23.720263696852076	29.51090402959597	22.161340937959203
49	21.068160003678422	23.05460348722036	32.22009989213795	23.65713661696327
50	20.04184204627531	33.23770075656704	26.840216643708466	19.88024055344919
51	17.917457750836746	35.002097849847594	23.96482126127714	23.115623138038522
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1327.0
1	1676.5
2	2026.0
3	4628.5
4	7231.0
5	5557.0
6	3883.0
7	4193.5
8	4504.0
9	4913.5
10	5323.0
11	5301.5
12	5280.0
13	5047.0
14	4814.0
15	4601.5
16	4389.0
17	4107.0
18	3825.0
19	3931.0
20	4037.0
21	4010.0
22	3983.0
23	4412.5
24	4842.0
25	5633.0
26	7001.5
27	7579.0
28	8887.5
29	10196.0
30	12019.0
31	13842.0
32	16285.5
33	18729.0
34	21259.5
35	23790.0
36	28950.0
37	34110.0
38	48526.5
39	62943.0
40	81312.0
41	99681.0
42	107947.0
43	116213.0
44	112119.5
45	108026.0
46	102580.0
47	97134.0
48	90390.5
49	83647.0
50	77398.0
51	71149.0
52	63783.0
53	56417.0
54	49703.5
55	42990.0
56	39021.0
57	35052.0
58	32258.0
59	29464.0
60	26550.5
61	23637.0
62	20233.5
63	16830.0
64	14151.0
65	11472.0
66	9288.5
67	7105.0
68	5835.5
69	4566.0
70	3769.0
71	2972.0
72	2550.0
73	2128.0
74	1753.5
75	1011.0
76	643.0
77	431.5
78	220.0
79	138.0
80	56.0
81	38.5
82	21.0
83	15.0
84	9.0
85	15.5
86	22.0
87	16.5
88	11.0
89	6.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1043926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.65060049726194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55917581614479	15.633855798544852
2	5.339752635003081	2.0985869156929464
3	1.7607832874057434	1.0380134682919746
4	0.9526200626992609	0.7487822511111919
5	0.6644334513000966	0.6528258154257572
6	0.5564646568656084	0.6560918797747213
7	0.466717528750686	0.6419895791784367
8	0.37708274076354265	0.5927921834525572
9	0.33909173153970207	0.5997020533570333
>10	7.386257307263336	38.40664054535162
>50	2.3133528476015504	30.957713085333843
>100	0.2823074661477116	7.310483337970373
>500	0.0	0.0
>1k	0.001960468514914664	0.6625230865146986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2583	0.24743133133957768	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1862	0.1783651331607796	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1382	0.13238486252857	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1052	0.10077342646892595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9158446096753984E-4	0.0	0.0	0.017146809256594817	0.0
2	1.9158446096753984E-4	0.0	0.0	0.05488894806720017	0.0
3	1.9158446096753984E-4	0.0	0.0	0.08094443475878559	0.0
4	1.9158446096753984E-4	0.0	0.0	0.12634995200809251	0.0
5	1.9158446096753984E-4	0.0	0.0	0.20346269754752733	0.0
6	1.9158446096753984E-4	0.0	0.0	0.2827786643880888	0.0
7	1.9158446096753984E-4	0.0	0.0	0.3232987778827235	0.0
8	1.9158446096753984E-4	0.0	0.0	0.40721277178650594	0.0
9	1.9158446096753984E-4	0.0	0.0	0.49764063736318476	0.0
10	1.9158446096753984E-4	0.0	0.0	0.677825822903156	0.0
11	1.9158446096753984E-4	0.0	0.0	0.9077271760642038	0.0
12	1.9158446096753984E-4	0.0	0.0	1.1102319513068934	0.0
13	1.9158446096753984E-4	0.0	0.0	1.1920385161400329	0.0
14	1.9158446096753984E-4	0.0	0.0	1.2247994589654823	0.0
15	1.9158446096753984E-4	0.0	0.0	1.280263160415585	0.0
16	1.9158446096753984E-4	0.0	0.0	1.3899452643195016	0.0
17	1.9158446096753984E-4	0.0	0.0	1.5255870626845198	0.0
18	1.9158446096753984E-4	0.0	0.0	1.7198537061056052	0.0
19	1.9158446096753984E-4	0.0	0.0	1.7970622438755237	0.0
20	1.9158446096753984E-4	0.0	0.0	1.876857171868504	0.0
21	1.9158446096753984E-4	0.0	0.0	1.985581353467583	0.0
22	1.9158446096753984E-4	0.0	0.0	2.0941139506056943	0.0
23	1.9158446096753984E-4	0.0	0.0	2.234162191572966	0.0
24	1.9158446096753984E-4	0.0	0.0	2.3259311483764176	0.0
25	1.9158446096753984E-4	0.0	0.0	2.394422593172313	0.0
26	1.9158446096753984E-4	0.0	0.0	2.469715286332556	0.0
27	1.9158446096753984E-4	0.0	0.0	2.5338002885261983	0.0
28	1.9158446096753984E-4	0.0	0.0	2.608422436073055	0.0
29	1.9158446096753984E-4	0.0	0.0	2.6855351816124897	0.0
30	1.9158446096753984E-4	0.0	0.0	2.7951214932859227	0.0
31	1.9158446096753984E-4	0.0	0.0	2.898577102208394	0.0
32	1.9158446096753984E-4	0.0	0.0	2.9858438241791085	0.0
33	1.9158446096753984E-4	0.0	0.0	3.073493715071758	0.0
34	1.9158446096753984E-4	0.0	0.0	3.164687918492307	0.0
35	1.9158446096753984E-4	0.0	0.0	3.2910378705003995	0.0
36	1.9158446096753984E-4	0.0	0.0	3.389033322285296	0.0
37	1.9158446096753984E-4	0.0	0.0	3.4861666439958388	0.0
38	1.9158446096753984E-4	0.0	0.0	3.582150458940576	0.0
39	1.9158446096753984E-4	0.0	0.0	3.6757394681232194	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.0329884E-4	45.000004	26
GATACGA	45	3.8562575E-10	45.000004	2
GGTACGC	20	7.0329884E-4	45.000004	45
GGTACGA	20	7.0329884E-4	45.000004	2
CGTATTT	20	7.0329884E-4	45.000004	23
TTAATCG	40	6.8139343E-9	45.000004	10
ACGTTGT	20	7.0329884E-4	45.000004	20
ACCGACA	20	7.0329884E-4	45.000004	27
CGACATA	20	7.0329884E-4	45.000004	29
AAGCGAT	20	7.0329884E-4	45.000004	45
CGTTCCC	20	7.0329884E-4	45.000004	19
TCGGCCA	20	7.0329884E-4	45.000004	13
GTTACAC	20	7.0329884E-4	45.000004	33
TTCCCGC	20	7.0329884E-4	45.000004	29
GCCGAAC	20	7.0329884E-4	45.000004	37
GTTAACG	20	7.0329884E-4	45.000004	27
TACGAAC	20	7.0329884E-4	45.000004	34
TCGTATT	20	7.0329884E-4	45.000004	22
AACTCCG	30	2.1653086E-6	45.000004	43
CGCACGA	30	2.1653086E-6	45.000004	39
>>END_MODULE