FastQCFastQC Report
Thu 26 May 2016
SRR1546923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546923_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1442650
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57420.398017537171178No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32700.22666620455411915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA351.2122109E-745.02
CTATCGT207.0338853E-445.037
CGTAGTC207.0338853E-445.018
TCGGTAT207.0338853E-445.026
CGCTTAT207.0338853E-445.018
CGTTTTT51600.042.950581
CGGTCTA2800.041.78571331
AGGGGAA143050.041.7284168
GAGGGGA142050.041.610357
CTAGCGA650.041.538462
TCGTTAG603.6379788E-1241.2500041
ACGTAAG556.184564E-1140.9090921
TATAGCG1050.040.7142871
GCGAAGA3250.040.1538472
CGTAAGA900.040.02
GTAACGA451.9288564E-840.02
AGACTTA10050.039.8507468
GAGACCG4300.039.767447
TTTAGCG1250.039.6000021
GTTGCGA1650.039.5454562
Produced by FastQC (version 0.11.5)