FastQCFastQC Report
Thu 26 May 2016
SRR1546922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546922_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences629436
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.33061343806201104No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.2257576624152416No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.030851E-445.00000439
CGAACAC207.030851E-445.00000444
ACGTTAG406.8066583E-945.0000041
GTTTACG600.045.0000041
TTCACGA406.8066583E-945.0000042
TCGCTCA207.030851E-445.00000429
GTCGTCA207.030851E-445.00000415
ACGTCGA302.1639298E-645.0000042
TATAGCG302.1639298E-645.0000041
CCGAAAT207.030851E-445.00000444
ATACGAA600.045.00000419
TTCGCGA302.1639298E-645.0000042
TGTAACG207.030851E-445.0000041
GTAACGA207.030851E-445.0000042
AGTCGAT207.030851E-445.00000443
TTGGCGA351.2107557E-745.02
CCGATCG253.8887883E-545.040
TACGCTA253.8887883E-545.01
GTAGCGA502.1827873E-1145.02
AGCGATA253.8887883E-545.08