##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546922_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	629436
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.220192362686596	33.0	31.0	34.0	31.0	34.0
2	32.61230847933706	34.0	31.0	34.0	31.0	34.0
3	32.633201786996615	34.0	31.0	34.0	31.0	34.0
4	36.253266416283786	37.0	35.0	37.0	35.0	37.0
5	36.267893796986506	37.0	35.0	37.0	35.0	37.0
6	36.31611633271691	37.0	37.0	37.0	35.0	37.0
7	36.41645377766763	37.0	37.0	37.0	35.0	37.0
8	14.700946561683793	0.0	0.0	37.0	0.0	37.0
9	25.9214312495631	19.0	17.0	39.0	17.0	39.0
10	33.61337451305613	32.0	32.0	39.0	27.0	39.0
11	36.32247281693453	37.0	35.0	39.0	34.0	39.0
12	37.23035701802884	39.0	37.0	39.0	35.0	39.0
13	37.33622798823073	39.0	37.0	39.0	35.0	39.0
14	38.62762695492473	40.0	38.0	41.0	35.0	41.0
15	38.91153826600321	40.0	38.0	41.0	35.0	41.0
16	39.01798912041892	40.0	38.0	41.0	35.0	41.0
17	39.02347339523001	40.0	38.0	41.0	35.0	41.0
18	38.98026646076805	40.0	38.0	41.0	35.0	41.0
19	38.95057956646903	40.0	38.0	41.0	35.0	41.0
20	38.912389821999376	40.0	38.0	41.0	35.0	41.0
21	38.847279151494355	40.0	38.0	41.0	35.0	41.0
22	38.797858717963386	40.0	38.0	41.0	35.0	41.0
23	38.69160168786024	40.0	38.0	41.0	35.0	41.0
24	38.595750799128105	40.0	37.0	41.0	35.0	41.0
25	38.50780063421857	40.0	37.0	41.0	35.0	41.0
26	38.465489740021226	40.0	37.0	41.0	35.0	41.0
27	38.43708335716419	40.0	37.0	41.0	35.0	41.0
28	38.41769615973665	40.0	37.0	41.0	35.0	41.0
29	38.452816489682824	40.0	37.0	41.0	35.0	41.0
30	38.38340037748079	40.0	37.0	41.0	35.0	41.0
31	38.31001881049066	40.0	37.0	41.0	35.0	41.0
32	38.14317420675017	40.0	37.0	41.0	34.0	41.0
33	38.00021288899904	40.0	37.0	41.0	34.0	41.0
34	37.872083897330306	40.0	37.0	41.0	34.0	41.0
35	37.74213105065487	40.0	36.0	41.0	34.0	41.0
36	37.644310144319675	40.0	36.0	41.0	34.0	41.0
37	37.56658500625957	40.0	36.0	41.0	33.0	41.0
38	37.508900031138985	40.0	36.0	41.0	33.0	41.0
39	37.46520694717175	40.0	36.0	41.0	33.0	41.0
40	37.3508172395605	40.0	36.0	41.0	33.0	41.0
41	37.33396564543496	40.0	36.0	41.0	33.0	41.0
42	37.284130554973025	40.0	36.0	41.0	33.0	41.0
43	37.19733856976722	40.0	35.0	41.0	33.0	41.0
44	37.014041141593424	40.0	35.0	41.0	33.0	41.0
45	36.93547874605202	40.0	35.0	41.0	33.0	41.0
46	36.90830203547303	40.0	35.0	41.0	33.0	41.0
47	36.867840415864364	40.0	35.0	41.0	33.0	41.0
48	36.8347107569316	40.0	35.0	41.0	32.0	41.0
49	36.78189680920697	40.0	35.0	41.0	32.0	41.0
50	36.66469188289199	39.0	35.0	41.0	32.0	41.0
51	36.27543229176596	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	2.0
14	2.0
15	1.0
16	17.0
17	52.0
18	68.0
19	130.0
20	272.0
21	524.0
22	921.0
23	1664.0
24	2728.0
25	4271.0
26	5841.0
27	6162.0
28	5653.0
29	5550.0
30	6096.0
31	7827.0
32	11126.0
33	17557.0
34	34305.0
35	66509.0
36	55143.0
37	117650.0
38	198040.0
39	81305.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.58833622481078	24.179900736532385	30.992666450600222	14.23909658805661
2	30.016236757986515	26.17184272904632	30.642193964120263	13.169726548846905
3	28.843281922228787	25.065773168360245	33.21830972489657	12.872635184514392
4	25.394956754936164	27.83396564543496	33.080885109844374	13.690192489784506
5	23.95557292560324	30.986788172268508	31.11086750678385	13.946771395344404
6	20.45211903990239	41.16987271144326	27.686055452818074	10.691952795836272
7	87.98257487655616	3.8793459541557835	6.730946434585883	1.4071327347021778
8	36.946091421526575	59.970036667746996	2.2769590554083337	0.8069128553180942
9	87.29926473859138	3.712529947445014	6.502170196811114	2.4860351171524986
10	49.01324360220896	25.694272332691487	13.716883050858227	11.575601014241322
11	42.18745035237895	26.005503339497583	19.571966014018898	12.235080294104565
12	37.24747234031736	23.680088205949453	24.976804631447834	14.095634822285346
13	20.553320750640257	38.035638253928916	26.428262762218875	14.982778233211954
14	14.970386186999155	40.627164636277556	29.528657401229037	14.873791775494253
15	13.933902731969571	25.402900374303343	45.626878665980335	15.036318227746746
16	16.512719323330728	22.160950438170044	43.81239713012919	17.513933108370033
17	17.243691177498587	21.71642549838268	28.889990404107806	32.14989292001093
18	21.299862098767786	25.365247618502913	34.094332068709136	19.24055821402017
19	25.878564302010055	26.663235023100047	27.56086401159133	19.897336663298574
20	28.9074663667156	25.376050940842276	26.091929918212497	19.624552774229628
21	22.64138053749706	27.849694011781978	30.22277086153318	19.28615458918778
22	22.599755972013043	24.129379317357124	28.776237774769793	24.49462693586004
23	19.380207042495186	28.908578473427006	28.041929600467718	23.66928488361009
24	19.481091008458364	25.84742531409071	37.06190939190005	17.609574285550874
25	17.59352817442917	26.58062138168138	36.247370661989464	19.578479781899986
26	16.524952497156185	35.06377137627972	28.725716355594532	19.685559770969565
27	17.422104868485437	33.73782243151011	31.395884569678252	17.444188130326197
28	14.752095526789061	29.35659860573593	39.29613177511296	16.59517409236205
29	16.211497276927282	25.725729065385522	38.00592911749566	20.056844540191538
30	17.941776447486323	30.084075267382225	33.66617098481816	18.307977300313297
31	28.189204303535227	26.23110212952548	26.66164629922661	18.91804726771268
32	27.695905540833383	27.638075991840317	27.991884798454493	16.674133668871814
33	25.423712657045357	27.974726580621383	27.209438290787308	19.392122471545957
34	19.79772367643414	29.216314287711537	28.393037576497054	22.592924459357267
35	19.568629693884684	27.1179277956774	30.86858711608488	22.444855394353038
36	27.80060244409281	25.162526452252493	28.884906487712808	18.15196461594189
37	20.43400758774522	31.169332545326295	29.630176856741592	18.766483010186896
38	19.810751212196315	32.927891000832496	24.80839990086363	22.452957886107562
39	19.97740834651974	30.440584904581243	28.502818396151476	21.07918835274754
40	23.155332710553576	26.85912467669469	26.64623567765428	23.339306935097454
41	17.860116040391716	25.27246614429426	29.258256597970245	27.609161217343782
42	22.126316257729144	26.71566291092343	26.073818466055325	25.084202365292104
43	22.41784708850463	26.71073786691578	26.855788356560478	24.015626688019115
44	19.439307570587	30.455995526153572	29.49831277524641	20.606384128013016
45	18.361835039622772	36.09056361568134	24.80919426280035	20.738407081895538
46	22.783730196556917	31.39318373909341	26.9655691762149	18.857516888134775
47	21.525460888795685	28.246080618204232	27.74197853316302	22.486479959837062
48	22.380829822253574	26.160245044770235	30.3227015931723	21.13622353980389
49	20.59589855044834	24.911349207862276	32.17976092883153	22.31299131285786
50	20.198717582089362	30.834906169968036	28.96767900151882	19.998697246423784
51	19.253109132620313	33.097884455290135	26.573789869025603	21.07521654306395
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	760.0
1	991.5
2	1223.0
3	3680.5
4	6138.0
5	4422.5
6	2707.0
7	2874.5
8	3042.0
9	3188.0
10	3334.0
11	3396.0
12	3458.0
13	3334.0
14	3210.0
15	3031.0
16	2852.0
17	2810.5
18	2769.0
19	2960.5
20	3152.0
21	3495.5
22	3839.0
23	4418.5
24	4998.0
25	5725.5
26	7549.0
27	8645.0
28	10008.5
29	11372.0
30	12938.0
31	14504.0
32	16506.5
33	18509.0
34	20165.0
35	21821.0
36	24343.5
37	26866.0
38	32876.5
39	38887.0
40	46974.0
41	55061.0
42	57465.5
43	59870.0
44	57566.0
45	55262.0
46	53877.5
47	52493.0
48	49359.5
49	46226.0
50	42384.0
51	38542.0
52	34964.0
53	31386.0
54	28392.5
55	25399.0
56	22948.0
57	20497.0
58	18642.0
59	16787.0
60	15258.0
61	13729.0
62	11626.5
63	9524.0
64	7695.5
65	5867.0
66	4878.5
67	3890.0
68	3272.5
69	2655.0
70	2186.0
71	1717.0
72	1370.5
73	1024.0
74	791.5
75	400.0
76	241.0
77	164.0
78	87.0
79	63.0
80	39.0
81	34.5
82	30.0
83	18.5
84	7.0
85	3.5
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	629436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.070715461632705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.09767751438966	16.873012499701627
2	5.523226128227175	2.658960091256233
3	2.7627419466092884	1.9950352587224833
4	1.920475085205434	1.8490883730853929
5	1.6266338961765294	1.957712083755612
6	1.3556373130137347	1.957869601843555
7	1.244246153188392	2.096492658234019
8	1.1975265777073278	2.30602572077887
9	1.075445777477204	2.3298074373661324
>10	13.005369009461724	61.880740668226906
>50	0.1664797577584382	2.521716982301908
>100	0.02122451094928296	0.751007070892572
>500	0.001989797901495277	0.26342722512175676
>1k	0.001326531934330185	0.5591043287129649
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2081	0.33061343806201104	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1421	0.2257576624152416	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007943619367179506	0.0
2	0.0	0.0	0.0	0.03272771179277957	0.0
3	0.0	0.0	0.0	0.04845607813979499	0.0
4	0.0	0.0	0.0	0.0886507921377233	0.0
5	0.0	0.0	0.0	0.17444188130326196	0.0
6	0.0	0.0	0.0	0.23894407056475955	0.0
7	0.0	0.0	0.0	0.28326946663362124	0.0
8	0.0	0.0	0.0	0.37096702444728297	0.0
9	0.0	0.0	0.0	0.440712002491119	0.0
10	0.0	0.0	0.0	0.6656753029696426	0.0
11	0.0	0.0	0.0	0.8625181908883508	0.0
12	0.0	0.0	0.0	1.0646038675893974	0.0
13	0.0	0.0	0.0	1.1311713978863618	0.0
14	0.0	0.0	0.0	1.1580208313474285	0.0
15	0.0	0.0	0.0	1.2067946542619106	0.0
16	0.0	0.0	0.0	1.2914736367160442	0.0
17	0.0	0.0	0.0	1.387273686284229	0.0
18	0.0	0.0	0.0	1.5226329603009678	0.0
19	0.0	0.0	0.0	1.5963497480283937	0.0
20	0.0	0.0	0.0	1.6743560902140964	0.0
21	0.0	0.0	0.0	1.7707916293316557	0.0
22	0.0	0.0	0.0	1.862937613990938	0.0
23	0.0	0.0	0.0	1.9839983731467536	0.0
24	0.0	0.0	0.0	2.0674063765021384	0.0
25	0.0	0.0	0.0	2.132385182925667	0.0
26	0.0	0.0	0.0	2.202130160969503	0.0
27	0.0	0.0	0.0	2.27235175617537	0.0
28	0.0	0.0	0.0	2.3503580983610726	0.0
29	0.0	0.0	0.0	2.4297942920328675	0.0
30	0.0	0.0	0.0	2.5254354692137087	0.0
31	0.0	0.0	0.0	2.6287025209870425	0.0
32	0.0	0.0	0.0	2.7157645892513296	0.0
33	0.0	0.0	0.0	2.80870493584733	0.0
34	0.0	0.0	0.0	2.9095889018105097	0.0
35	0.0	0.0	0.0	3.0339859811005407	0.0
36	0.0	0.0	0.0	3.1356643090004384	0.0
37	0.0	0.0	0.0	3.240520084647208	0.0
38	0.0	0.0	0.0	3.347123456554757	0.0
39	0.0	0.0	0.0	3.4434001232849725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.030851E-4	45.000004	39
CGAACAC	20	7.030851E-4	45.000004	44
ACGTTAG	40	6.8066583E-9	45.000004	1
GTTTACG	60	0.0	45.000004	1
TTCACGA	40	6.8066583E-9	45.000004	2
TCGCTCA	20	7.030851E-4	45.000004	29
GTCGTCA	20	7.030851E-4	45.000004	15
ACGTCGA	30	2.1639298E-6	45.000004	2
TATAGCG	30	2.1639298E-6	45.000004	1
CCGAAAT	20	7.030851E-4	45.000004	44
ATACGAA	60	0.0	45.000004	19
TTCGCGA	30	2.1639298E-6	45.000004	2
TGTAACG	20	7.030851E-4	45.000004	1
GTAACGA	20	7.030851E-4	45.000004	2
AGTCGAT	20	7.030851E-4	45.000004	43
TTGGCGA	35	1.2107557E-7	45.0	2
CCGATCG	25	3.8887883E-5	45.0	40
TACGCTA	25	3.8887883E-5	45.0	1
GTAGCGA	50	2.1827873E-11	45.0	2
AGCGATA	25	3.8887883E-5	45.0	8
>>END_MODULE