FastQCFastQC Report
Thu 26 May 2016
SRR1546921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546921_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences904172
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96491.067164212118933No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53430.5909273899213866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG302.1649867E-645.0000041
CGCATCG406.8121153E-945.021
TTGTACG700.045.01
CGTTTTT71150.043.418831
TGTACGA1150.043.0434762
CGGTCTA1750.042.4285731
TAGAGTA2450.042.24495
GTTTTTA49600.042.006052
ATTACGA451.927583E-840.0000042
GCGTAAG850.039.7058831
CGCATGA403.457135E-739.3752
CGAGGTA403.457135E-739.3756
TATTTTT55600.039.2940647
TATGCGA750.039.02
TTATTTT56000.038.9330376
CACGACG1850.038.9189226
AGGGGAA48000.038.6718758
CTATGCG356.2468116E-638.5714261
CGTACGA356.2468116E-638.5714262
GCCCGAA1000.038.2525
Produced by FastQC (version 0.11.5)