##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546921_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	904172
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.725766778887202	31.0	31.0	34.0	30.0	34.0
2	32.08819671478435	33.0	31.0	34.0	30.0	34.0
3	32.06477528611813	33.0	31.0	34.0	30.0	34.0
4	35.853793304813685	37.0	35.0	37.0	35.0	37.0
5	35.85960967603509	37.0	35.0	37.0	35.0	37.0
6	35.91094725339869	37.0	35.0	37.0	35.0	37.0
7	36.10403440938229	37.0	35.0	37.0	35.0	37.0
8	13.241876545613003	0.0	0.0	35.0	0.0	37.0
9	24.93354251182297	18.0	17.0	39.0	17.0	39.0
10	32.747576788487144	32.0	32.0	38.0	27.0	39.0
11	35.6108450604531	37.0	35.0	37.0	32.0	39.0
12	36.660122189141006	38.0	35.0	39.0	33.0	39.0
13	36.886225187243134	39.0	35.0	39.0	33.0	39.0
14	37.998422866445765	40.0	37.0	41.0	33.0	41.0
15	38.29102538012679	40.0	37.0	41.0	33.0	41.0
16	38.38809761859469	40.0	37.0	41.0	34.0	41.0
17	38.35929225855258	40.0	37.0	41.0	34.0	41.0
18	38.2809620293484	40.0	37.0	41.0	34.0	41.0
19	38.2232351809169	40.0	37.0	41.0	34.0	41.0
20	38.149457182925374	40.0	36.0	41.0	34.0	41.0
21	38.10047645801905	40.0	36.0	41.0	33.0	41.0
22	38.07039921607836	40.0	36.0	41.0	33.0	41.0
23	37.921270510478095	40.0	36.0	41.0	33.0	41.0
24	37.73753555739395	40.0	35.0	41.0	33.0	41.0
25	37.62705547174652	40.0	35.0	41.0	33.0	41.0
26	37.56082692231124	40.0	35.0	41.0	33.0	41.0
27	37.52611781829121	40.0	35.0	41.0	33.0	41.0
28	37.43625770318037	39.0	35.0	41.0	33.0	41.0
29	37.382346500444605	39.0	35.0	41.0	33.0	41.0
30	37.23888928212774	39.0	35.0	41.0	33.0	41.0
31	37.08001574921586	39.0	35.0	41.0	32.0	41.0
32	36.84925655738068	39.0	35.0	41.0	31.0	41.0
33	36.5863298133541	39.0	35.0	41.0	31.0	41.0
34	36.28210782904138	39.0	35.0	41.0	30.0	41.0
35	36.05297664603637	39.0	35.0	41.0	29.0	41.0
36	35.90802081904771	39.0	35.0	41.0	27.0	41.0
37	35.86144007998478	39.0	35.0	41.0	27.0	41.0
38	35.75737027910619	39.0	35.0	41.0	27.0	41.0
39	35.65940772330928	39.0	35.0	41.0	26.0	41.0
40	35.54300509195153	39.0	35.0	41.0	26.0	41.0
41	35.44052016651699	39.0	35.0	41.0	25.0	41.0
42	35.386757165672016	39.0	35.0	40.0	25.0	41.0
43	35.277313387275875	38.0	35.0	40.0	24.0	41.0
44	35.04375716124808	38.0	34.0	40.0	23.0	41.0
45	34.98854974496003	38.0	34.0	40.0	23.0	41.0
46	34.98014758253961	38.0	34.0	40.0	23.0	41.0
47	34.9314101741704	38.0	34.0	40.0	23.0	41.0
48	34.85414390182399	38.0	34.0	40.0	23.0	41.0
49	34.74847263573745	38.0	34.0	40.0	24.0	41.0
50	34.64172303499777	37.0	34.0	40.0	24.0	41.0
51	34.29751640174657	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	4.0
14	5.0
15	16.0
16	72.0
17	135.0
18	314.0
19	590.0
20	1184.0
21	1858.0
22	3122.0
23	5044.0
24	7901.0
25	12193.0
26	15743.0
27	16947.0
28	16716.0
29	17131.0
30	19613.0
31	23815.0
32	30332.0
33	42291.0
34	64388.0
35	94206.0
36	96936.0
37	158618.0
38	194848.0
39	80127.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.42881664108157	22.8741876545613	25.681949894489104	18.01504580986803
2	36.27263396787337	23.115402821587043	28.274266400640585	12.337696809899
3	29.289891746260665	23.417225926040622	34.60790646027526	12.684975867423454
4	25.738355091730337	25.692456744955606	34.11408448834956	14.455103674964498
5	22.411222643479338	30.04074446012484	33.09536238680251	14.452670509593307
6	21.427339046110696	38.43317421906451	30.34710210004291	9.792384634781879
7	82.66989024212208	3.9106497436328485	11.456448551824211	1.9630114624208668
8	31.228460956543667	63.6261684723703	4.1653579186261025	0.9800126524599303
9	79.44008440871869	4.724432961870087	12.47306928327796	3.3624133461332577
10	46.53096977123822	23.46588923346443	18.23038094521839	11.772760050078968
11	38.315497493839665	21.62232407108382	25.09566763845817	14.966510796618342
12	35.41472197767682	19.471074087673585	29.182611273076365	15.93159266157324
13	26.221117221059707	25.449361404688485	30.139177059232093	18.190344315019708
14	19.089067124396685	27.53856567113337	34.03146746415505	19.340899740314896
15	18.925049658693258	22.01948301871768	41.09903867848153	17.956428644107536
16	24.83067380985034	19.44021712682985	37.57128068553328	18.15782837778653
17	24.203580734638987	20.554938662112963	30.81095189853258	24.430528704715474
18	25.758926398959492	22.29078095760541	33.0614086700318	18.88888397340329
19	27.584353419482134	24.731245824909422	28.791424640444518	18.89297611516393
20	28.01557668231266	25.363868821418933	28.32757484195485	18.29297965431356
21	26.12091504713705	25.228164552762085	30.98027808868224	17.670642311418625
22	24.322916436253276	21.7398901978827	32.417283437222125	21.519909928641894
23	22.877505607340197	24.163544104440305	31.93430011104082	21.02465017717868
24	24.128926797113824	22.969965891445433	34.452405073371	18.448702238069746
25	24.095857867750826	22.64447472383573	31.907756488809653	21.351910919603792
26	20.355419101675345	26.15940329937224	30.318899501422298	23.166278097530117
27	19.444862260720306	24.922470503399797	34.28020332414629	21.352463911733608
28	18.88822038284751	24.51922864233796	35.21918396057387	21.373367014240653
29	20.808762049698508	25.772972399056815	33.435452546639354	19.98281300460532
30	23.68631189640909	24.137664072764917	33.13141747366652	19.04460655715948
31	28.198285282003866	22.714041133766585	29.87141826997518	19.21625531425437
32	28.983091712638746	23.540432572563628	28.906668200298174	18.569807514499452
33	27.83076671252815	23.97917652835965	28.323482700194212	19.866574058917994
34	22.41055905292356	25.28169419092827	31.301787712957267	21.0059590431909
35	21.887317899691652	26.89366624934194	31.003835553412408	20.215180297554006
36	26.254739142552523	27.50947828510505	27.4567228359206	18.779059736421832
37	23.521077848020067	29.3345735103498	27.0726145025504	20.071734139079734
38	23.84236627544317	29.662608441756653	25.744769800436202	20.750255482363976
39	23.11319085306778	27.444778205916577	26.63044199554952	22.811588945466127
40	25.115575355131547	24.26595824688223	27.244373858071253	23.374092539914972
41	21.151064178054618	24.23001375844419	28.743646120428412	25.87527594307278
42	22.01716045177245	25.541932287219687	27.327433275969614	25.113473985038244
43	21.569568621899375	24.503081272147334	27.878987626248104	26.04836247970519
44	21.056613122282045	26.05533018054087	29.252730675137034	23.635326022040054
45	21.836995615878394	28.337528700291536	26.65742801148454	23.168047672345526
46	23.50570466681118	27.175581637122143	27.809089421039364	21.509624275027317
47	21.703724512592736	26.274204465522043	29.424158235379995	22.597912786505223
48	21.56072074782232	25.359113089102514	31.119410908543948	21.960755254531218
49	23.559676698681226	23.704118242989168	30.970324230345554	21.765880827984056
50	20.860743309901213	26.084970558699013	30.36369186393739	22.690594267462387
51	20.147162265586633	27.52684223798127	29.849740978486395	22.4762545179457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	608.0
1	1427.5
2	2247.0
3	13817.5
4	25388.0
5	16677.0
6	7966.0
7	7652.0
8	7338.0
9	7251.0
10	7164.0
11	6959.5
12	6755.0
13	6402.5
14	6050.0
15	5726.5
16	5403.0
17	4991.0
18	4579.0
19	4490.0
20	4401.0
21	4443.0
22	4485.0
23	4831.5
24	5178.0
25	5790.0
26	7191.0
27	7980.0
28	9590.5
29	11201.0
30	12767.0
31	14333.0
32	15973.5
33	17614.0
34	18993.0
35	20372.0
36	23233.0
37	26094.0
38	31635.5
39	37177.0
40	42592.5
41	48008.0
42	50948.0
43	53888.0
44	55067.5
45	56247.0
46	56839.5
47	57432.0
48	57443.0
49	57454.0
50	57025.0
51	56596.0
52	55244.0
53	53892.0
54	53789.0
55	53686.0
56	53408.0
57	53130.0
58	50770.5
59	48411.0
60	44869.5
61	41328.0
62	36606.5
63	31885.0
64	27197.0
65	22509.0
66	18691.5
67	14874.0
68	12619.0
69	10364.0
70	8591.0
71	6818.0
72	5526.0
73	4234.0
74	3348.0
75	1871.5
76	1281.0
77	911.5
78	542.0
79	377.5
80	213.0
81	145.5
82	78.0
83	58.0
84	38.0
85	35.0
86	32.0
87	23.5
88	15.0
89	11.0
90	7.0
91	7.0
92	7.0
93	5.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	904172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.7848859117952
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.74467294778199	22.88746046672693
2	7.821302070782686	5.284835962861612
3	4.613421956538779	4.675918033939008
4	3.41817693431987	4.619308710093066
5	2.658679788217454	4.491159666046125
6	2.182889974693429	4.42492132518114
7	1.784275777933802	4.2197087514871185
8	1.4349956431690505	3.8784931270711627
9	1.2131682206276346	3.68880749331482
>10	7.06095415186318	37.76750702044726
>50	0.04738841220677995	1.065783498856539
>100	0.019415953935373476	1.3292757572722766
>500	0.0	0.0
>1k	0.0	0.0
>5k	6.581679299609191E-4	1.6668201867029238
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9649	1.067164212118933	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5343	0.5909273899213866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0050875275943072775	0.0
2	0.0	0.0	0.0	0.013493007967510606	0.0
3	0.0	0.0	0.0	0.02300447260034595	0.0
4	0.0	0.0	0.0	0.03649748056785656	0.0
5	0.0	0.0	0.0	0.06989820520874347	0.0
6	0.0	0.0	0.0	0.12740938670960836	0.0
7	0.0	0.0	0.0	0.17441371774396908	0.0
8	0.0	0.0	0.0	0.2498418442508726	0.0
9	0.0	0.0	0.0	0.4036842547656862	0.0
10	0.0	0.0	0.0	0.6166968231708126	0.0
11	0.0	0.0	0.0	0.8074791079573356	0.0
12	0.0	0.0	0.0	0.9436257703180368	0.0
13	0.0	0.0	0.0	1.008878841636326	0.0
14	0.0	0.0	0.0	1.0536712041514225	0.0
15	0.0	0.0	0.0	1.090721676849095	0.0
16	0.0	0.0	0.0	1.1440301181633583	0.0
17	0.0	0.0	0.0	1.1991081342930328	0.0
18	0.0	0.0	0.0	1.2799555836721332	0.0
19	0.0	0.0	0.0	1.3279553005401628	0.0
20	0.0	0.0	0.0	1.3863512694487332	0.0
21	0.0	0.0	0.0	1.4561388762315135	0.0
22	0.0	0.0	0.0	1.5280278531075946	0.0
23	0.0	0.0	0.0	1.6086541056347685	0.0
24	0.0	0.0	0.0	1.6764509407502113	0.0
25	0.0	0.0	0.0	1.7325243427135546	0.0
26	0.0	0.0	0.0	1.7936852722712051	0.0
27	0.0	0.0	0.0	1.8722101547050782	0.0
28	0.0	0.0	0.0	1.940117588246484	0.0
29	0.0	0.0	0.0	2.0273797463314502	0.0
30	0.0	0.0	0.0	2.134881416367682	0.0
31	0.0	0.0	0.0	2.2156182673208193	0.0
32	0.0	0.0	0.0	2.294253748180656	0.0
33	0.0	0.0	0.0	2.37764496135691	0.0
34	0.0	0.0	0.0	2.479284914817092	0.0
35	0.0	0.0	0.0	2.593754285689006	0.0
36	0.0	0.0	0.0	2.695173042297262	0.0
37	0.0	0.0	0.0	2.8088682241874334	0.0
38	0.0	0.0	0.0	2.995779564065244	0.0
39	0.0	0.0	0.0	3.310763881208443	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	30	2.1649867E-6	45.000004	1
CGCATCG	40	6.8121153E-9	45.0	21
TTGTACG	70	0.0	45.0	1
CGTTTTT	7115	0.0	43.41883	1
TGTACGA	115	0.0	43.043476	2
CGGTCTA	175	0.0	42.42857	31
TAGAGTA	245	0.0	42.2449	5
GTTTTTA	4960	0.0	42.00605	2
ATTACGA	45	1.927583E-8	40.000004	2
GCGTAAG	85	0.0	39.705883	1
CGCATGA	40	3.457135E-7	39.375	2
CGAGGTA	40	3.457135E-7	39.375	6
TATTTTT	5560	0.0	39.294064	7
TATGCGA	75	0.0	39.0	2
TTATTTT	5600	0.0	38.933037	6
CACGACG	185	0.0	38.91892	26
AGGGGAA	4800	0.0	38.671875	8
CTATGCG	35	6.2468116E-6	38.571426	1
CGTACGA	35	6.2468116E-6	38.571426	2
GCCCGAA	100	0.0	38.25	25
>>END_MODULE