FastQCFastQC Report
Thu 26 May 2016
SRR1546920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546920_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences890952
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.3147195359570437No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.17644048164210865No Hit
AAGGAAGAAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9390.10539288311828246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT253.8900966E-545.00000425
ATTTACG351.2115197E-745.0000041
TTTAGCG351.2115197E-745.0000041
AGCCGAC207.032431E-445.00000432
TCGTTAG351.2115197E-745.0000041
CGAACGT253.8900966E-545.00000437
GATCGTA207.032431E-445.00000422
GTATTCG302.1649503E-645.0000041
CACGGCG207.032431E-445.00000426
CTATCGT302.1649503E-645.00000413
ACGTTAG302.1649503E-645.0000041
ACCATAT207.032431E-445.00000445
AACCCGA302.1649503E-645.00000429
GCGAAAA351.2115197E-745.00000422
CGGTTGA207.032431E-445.0000042
ATGTGAC351.2115197E-745.00000443
TAGCGAA253.8900966E-545.0000042
CGTCTTA207.032431E-445.00000431
CACGACG1150.045.00000426
GCTTGCA207.032431E-445.00000430