##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546920_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	890952
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80077826863849	31.0	31.0	34.0	30.0	34.0
2	32.13889973870646	33.0	31.0	34.0	30.0	34.0
3	32.15013042228986	33.0	31.0	34.0	30.0	34.0
4	35.87060470148785	37.0	35.0	37.0	35.0	37.0
5	35.859085562409646	37.0	35.0	37.0	35.0	37.0
6	35.89941433433002	37.0	35.0	37.0	35.0	37.0
7	36.127171834172884	37.0	35.0	37.0	35.0	37.0
8	13.215856746491394	0.0	0.0	36.0	0.0	37.0
9	24.950400246028966	18.0	17.0	39.0	17.0	39.0
10	32.84578518259121	32.0	32.0	38.0	27.0	39.0
11	35.68845010730095	37.0	35.0	37.0	32.0	39.0
12	36.46469619014268	37.0	35.0	39.0	33.0	39.0
13	36.449971491168995	39.0	35.0	39.0	33.0	39.0
14	37.60767920157315	39.0	36.0	41.0	33.0	41.0
15	38.03351022277295	40.0	36.0	41.0	33.0	41.0
16	38.21297219154343	40.0	36.0	41.0	34.0	41.0
17	38.194227073961336	40.0	36.0	41.0	34.0	41.0
18	38.15612962314468	40.0	36.0	41.0	34.0	41.0
19	38.11114291230055	40.0	36.0	41.0	34.0	41.0
20	38.071136267722615	40.0	36.0	41.0	34.0	41.0
21	37.96128410958166	40.0	36.0	41.0	34.0	41.0
22	37.903536890876275	40.0	35.0	41.0	33.0	41.0
23	37.84921634386589	40.0	35.0	41.0	34.0	41.0
24	37.77264880711868	40.0	35.0	41.0	33.0	41.0
25	37.66151599637242	40.0	35.0	41.0	33.0	41.0
26	37.56651312304142	40.0	35.0	41.0	33.0	41.0
27	37.460302014025444	39.0	35.0	41.0	33.0	41.0
28	37.45540388258851	39.0	35.0	41.0	33.0	41.0
29	37.51635329400462	40.0	35.0	41.0	33.0	41.0
30	37.439752085409765	40.0	35.0	41.0	33.0	41.0
31	37.31760072372025	40.0	35.0	41.0	33.0	41.0
32	37.1547715252898	39.0	35.0	41.0	33.0	41.0
33	37.03979338954287	40.0	35.0	41.0	32.0	41.0
34	36.91203342043118	40.0	35.0	41.0	32.0	41.0
35	36.819763578733756	40.0	35.0	41.0	32.0	41.0
36	36.67592417997827	39.0	35.0	41.0	31.0	41.0
37	36.54005378516463	39.0	35.0	41.0	31.0	41.0
38	36.48690052887249	39.0	35.0	41.0	31.0	41.0
39	36.456512808770846	39.0	35.0	41.0	31.0	41.0
40	36.31197191318949	39.0	35.0	41.0	30.0	41.0
41	36.296251649920535	39.0	35.0	41.0	30.0	41.0
42	36.237609882462806	39.0	35.0	41.0	30.0	41.0
43	36.12063837333549	39.0	35.0	41.0	30.0	41.0
44	35.93732322279988	38.0	35.0	40.0	30.0	41.0
45	35.82016876330038	38.0	35.0	40.0	29.0	41.0
46	35.81113348418321	38.0	35.0	40.0	30.0	41.0
47	35.78016773069705	38.0	35.0	40.0	29.0	41.0
48	35.72222746006519	38.0	35.0	40.0	29.0	41.0
49	35.64427825516975	38.0	35.0	40.0	29.0	41.0
50	35.5155025186542	38.0	35.0	40.0	29.0	41.0
51	35.07981799243955	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	24.0
16	31.0
17	98.0
18	220.0
19	451.0
20	850.0
21	1448.0
22	2341.0
23	3571.0
24	5432.0
25	7879.0
26	10128.0
27	11443.0
28	12165.0
29	14307.0
30	17365.0
31	22417.0
32	30027.0
33	42548.0
34	69911.0
35	116101.0
36	90218.0
37	153097.0
38	212514.0
39	66328.0
40	30.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.62773752121326	24.252933940324507	28.3724600202929	13.746868518169327
2	31.464994747191767	25.71765931273514	30.130130467185662	12.687215472887429
3	30.54036581095278	24.930187035889702	31.57397929405849	12.955467859099032
4	26.68336790309691	28.305565282978208	29.89319289928077	15.117873914644111
5	24.349459903563826	31.567244924530165	29.261733516508187	14.821561655397822
6	21.622376963068717	41.09301062234554	26.75239519076224	10.532217223823507
7	87.06417405202525	4.903294453573256	6.363081288329786	1.6694502060717078
8	32.98269715989189	63.80579425154217	2.2779004929558493	0.933608095610089
9	85.1437563415313	5.0741229606084275	7.31509666065063	2.4670240372096366
10	60.45656780612199	17.862802934389283	11.45819303396816	10.222436225520566
11	52.915869766272486	18.483824044392964	15.677612261940038	12.92269392739452
12	45.858362740080274	21.077341989242967	19.803648232452478	13.260647038224283
13	21.102932593450603	43.076619166913595	21.744044572547118	14.076403667088687
14	14.402122673275327	45.85140389156767	26.523875584767755	13.222597850389247
15	13.35380581669944	22.452051288958327	50.5509836669091	13.643159227433127
16	16.49639935709219	16.404362973538415	48.64178990562903	18.45744776374036
17	16.606169580403883	16.945694044123588	27.511021918128026	38.9371144573445
18	22.60851314099974	22.665194084529805	34.21912740529231	20.50716536917814
19	30.717704208532	24.56619436288375	23.504184288266934	21.211917140317325
20	33.77263870556438	23.640330792231232	22.356984439116808	20.230046063087574
21	23.680175811940487	27.511358636604445	27.845383365209347	20.963082186245725
22	23.68859377385089	23.50530668318832	24.715921845396835	28.090177697563956
23	19.92598927888371	30.321498801282225	23.194291050471854	26.55822086936221
24	21.2196616652749	23.674339358349272	37.46071617775144	17.645282798624393
25	18.390440786933524	24.249678995052484	35.98790956190682	21.371970656107177
26	16.762631432445293	35.30134058849411	26.49839722005226	21.437630759008343
27	18.471140981781286	34.06434914563299	28.993481130296583	18.471028742289146
28	15.242796469394534	29.390359974499187	39.086168502904755	16.280675053201517
29	16.358905979222225	24.658230746437518	37.97881367346389	21.004049600876364
30	19.86605339008162	29.965026174249566	31.485422334761022	18.683498100907794
31	32.61881672637807	26.181769612728857	22.105231258249603	19.094182402643465
32	32.546758972424996	26.987985884761468	24.831977480268296	15.633277662545234
33	29.29821135145328	28.440926110497532	23.138844741355314	19.122017796693875
34	20.143172696172186	29.338842047607507	26.46854151514335	24.04944374107696
35	20.41860840988067	26.365168942883564	30.120028912893176	23.09619373434259
36	33.45208271601612	23.792190825094956	24.93748260287872	17.8182438560102
37	22.094231788020004	32.38749113308012	27.17856854241306	18.339708536486814
38	21.906904075640437	33.89991828964972	20.64106708329966	23.552110551410177
39	21.798256247250134	31.436598155680667	25.910374520737367	20.854771076331836
40	24.457209816017027	26.277397660031067	23.246819132792787	26.018573391159123
41	18.089975666478104	23.461869999730624	26.692347062468013	31.75580727132326
42	23.313489391123206	26.132384236187807	23.21707566737602	27.337050705312972
43	23.487797322414675	25.51046521024702	25.171052986019447	25.830684481318862
44	20.015780872594707	30.366057879661305	27.04186084098807	22.57630040675592
45	18.45621312932683	37.79092476362363	21.90746527310113	21.845396833948406
46	24.893933679928885	31.78308146791297	24.757113739011753	18.565871113146386
47	23.734387486643502	27.141080552038716	25.218754770178414	23.905777191139364
48	24.997755210157223	23.85987123885462	29.118628164031286	22.023745386956872
49	21.771879966597528	23.03446201366628	31.587111314638722	23.606546705097468
50	21.188459086460327	32.30331151397606	26.19591178873834	20.312317610825275
51	19.838891432984042	35.170132622183914	22.460357011376594	22.53061893345545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1071.0
1	1407.5
2	1744.0
3	4705.5
4	7667.0
5	5586.5
6	3506.0
7	3723.0
8	3940.0
9	4079.5
10	4219.0
11	4283.5
12	4348.0
13	4118.5
14	3889.0
15	3754.0
16	3619.0
17	3368.0
18	3117.0
19	3083.0
20	3049.0
21	3000.5
22	2952.0
23	3074.5
24	3197.0
25	3837.5
26	5657.0
27	6836.0
28	8215.5
29	9595.0
30	10811.0
31	12027.0
32	12531.5
33	13036.0
34	13382.5
35	13729.0
36	18242.5
37	22756.0
38	34217.0
39	45678.0
40	60843.0
41	76008.0
42	84265.5
43	92523.0
44	89263.5
45	86004.0
46	81450.5
47	76897.0
48	72986.5
49	69076.0
50	64804.5
51	60533.0
52	55367.0
53	50201.0
54	47099.0
55	43997.0
56	41837.0
57	39677.0
58	37171.0
59	34665.0
60	30993.0
61	27321.0
62	24072.5
63	20824.0
64	17174.5
65	13525.0
66	11164.5
67	8804.0
68	7423.5
69	6043.0
70	5133.0
71	4223.0
72	3524.5
73	2826.0
74	2354.5
75	1336.0
76	789.0
77	578.5
78	368.0
79	288.0
80	208.0
81	141.0
82	74.0
83	46.5
84	19.0
85	10.5
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	890952.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.9232471119191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89048265122297	16.29721816178186
2	5.016652305670116	2.0992931173222913
3	1.807715309552238	1.1346982238928243
4	1.0952552707110752	0.916651867188787
5	0.8329119501495141	0.8713611277724364
6	0.6369854296406898	0.7996682130638467
7	0.5536906025539438	0.8109503710548485
8	0.5171025640949188	0.8655571784611977
9	0.47515308068813616	0.8947570790904756
>10	9.526212521404736	49.871267085736235
>50	1.5171774781178946	20.546569495428223
>100	0.12580154889731712	3.8972033695843886
>500	0.0037794456750266945	0.5004525168780463
>1k	0.0010798416214361985	0.49435219274454417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2804	0.3147195359570437	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1572	0.17644048164210865	No Hit
AAGGAAGAAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	939	0.10539288311828246	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1223949213874597E-4	0.0	0.0	0.012683062611678295	0.0
2	1.1223949213874597E-4	0.0	0.0	0.03804918783503489	0.0
3	1.1223949213874597E-4	0.0	0.0	0.04960985552532572	0.0
4	1.1223949213874597E-4	0.0	0.0	0.07924108144995466	0.0
5	1.1223949213874597E-4	0.0	0.0	0.14007488618915498	0.0
6	1.1223949213874597E-4	0.0	0.0	0.19731702717991542	0.0
7	1.1223949213874597E-4	0.0	0.0	0.23458053856997907	0.0
8	1.1223949213874597E-4	0.0	0.0	0.3232497373595884	0.0
9	1.1223949213874597E-4	0.0	0.0	0.42594887266654097	0.0
10	1.1223949213874597E-4	0.0	0.0	0.6060932575492283	0.0
11	1.1223949213874597E-4	0.0	0.0	0.8378678088157386	0.0
12	1.1223949213874597E-4	0.0	0.0	1.0240731262739182	0.0
13	1.1223949213874597E-4	0.0	0.0	1.104099884168844	0.0
14	1.1223949213874597E-4	0.0	0.0	1.1340678285698893	0.0
15	1.1223949213874597E-4	0.0	0.0	1.1804227388231914	0.0
16	1.1223949213874597E-4	0.0	0.0	1.2826729161615888	0.0
17	1.1223949213874597E-4	0.0	0.0	1.3915452235361725	0.0
18	1.1223949213874597E-4	0.0	0.0	1.5594555037757365	0.0
19	1.1223949213874597E-4	0.0	0.0	1.6302786233152853	0.0
20	1.1223949213874597E-4	0.0	0.0	1.712999129021541	0.0
21	1.1223949213874597E-4	0.0	0.0	1.8208612809668758	0.0
22	1.1223949213874597E-4	0.0	0.0	1.9267031220537134	0.0
23	1.1223949213874597E-4	0.0	0.0	2.0485952105163916	0.0
24	1.1223949213874597E-4	0.0	0.0	2.1396214386409143	0.0
25	1.1223949213874597E-4	0.0	0.0	2.2077508103691335	0.0
26	1.1223949213874597E-4	0.0	0.0	2.282839030609954	0.0
27	1.1223949213874597E-4	0.0	0.0	2.345356427731236	0.0
28	1.1223949213874597E-4	0.0	0.0	2.418648816097837	0.0
29	1.1223949213874597E-4	0.0	0.0	2.4986755739927626	0.0
30	1.1223949213874597E-4	0.0	0.0	2.601935906760409	0.0
31	1.1223949213874597E-4	0.0	0.0	2.7086756637843563	0.0
32	1.1223949213874597E-4	0.0	0.0	2.8037425136258745	0.0
33	1.1223949213874597E-4	0.0	0.0	2.8981359265145596	0.0
34	1.1223949213874597E-4	0.0	0.0	2.9911824654975803	0.0
35	1.1223949213874597E-4	0.0	0.0	3.1403487505499736	0.0
36	1.1223949213874597E-4	0.0	0.0	3.2489965789402797	0.0
37	1.1223949213874597E-4	0.0	0.0	3.348777487451625	0.0
38	1.1223949213874597E-4	0.0	0.0	3.451476622758577	0.0
39	1.1223949213874597E-4	0.0	0.0	3.563716114897323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCGT	25	3.8900966E-5	45.000004	25
ATTTACG	35	1.2115197E-7	45.000004	1
TTTAGCG	35	1.2115197E-7	45.000004	1
AGCCGAC	20	7.032431E-4	45.000004	32
TCGTTAG	35	1.2115197E-7	45.000004	1
CGAACGT	25	3.8900966E-5	45.000004	37
GATCGTA	20	7.032431E-4	45.000004	22
GTATTCG	30	2.1649503E-6	45.000004	1
CACGGCG	20	7.032431E-4	45.000004	26
CTATCGT	30	2.1649503E-6	45.000004	13
ACGTTAG	30	2.1649503E-6	45.000004	1
ACCATAT	20	7.032431E-4	45.000004	45
AACCCGA	30	2.1649503E-6	45.000004	29
GCGAAAA	35	1.2115197E-7	45.000004	22
CGGTTGA	20	7.032431E-4	45.000004	2
ATGTGAC	35	1.2115197E-7	45.000004	43
TAGCGAA	25	3.8900966E-5	45.000004	2
CGTCTTA	20	7.032431E-4	45.000004	31
CACGACG	115	0.0	45.000004	26
GCTTGCA	20	7.032431E-4	45.000004	30
>>END_MODULE