FastQCFastQC Report
Thu 26 May 2016
SRR1546919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546919_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences899318
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38190.42465512755221174No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21580.23995961384071038No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10700.1189790485679148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT351.2115379E-745.0000044
TCGATAG302.164972E-645.0000041
GCTACGA302.164972E-645.0000042
GCGTAAG302.164972E-645.0000041
TACCCGT253.890127E-545.030
TTAGCGA1050.045.02
TATGCGA800.045.02
ACGGGTA207.0324674E-445.05
TAGCCGT1000.045.044
TCATACG253.890127E-545.01
GTAACGA253.890127E-545.02
CGGTCTA1850.043.78378331
CGTTTTT33300.043.5135151
TAACGCC1050.042.85714312
ATAACGC1050.042.85714311
CACGACG1950.042.69230726
AGACTTA7400.041.9594578
TCACGAC2000.041.62525
TAAGCGA650.041.5384642
GAGGGGA78700.041.0260477