##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546919_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	899318
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.723497138943067	31.0	31.0	34.0	30.0	34.0
2	32.0734234164111	33.0	31.0	34.0	30.0	34.0
3	32.11117313341888	33.0	31.0	34.0	30.0	34.0
4	35.85679036781205	37.0	35.0	37.0	35.0	37.0
5	35.83891237582257	37.0	35.0	37.0	35.0	37.0
6	35.8862882762271	37.0	35.0	37.0	35.0	37.0
7	36.10693214191198	37.0	35.0	37.0	35.0	37.0
8	13.299618155090858	0.0	0.0	36.0	0.0	37.0
9	24.9961871106772	18.0	17.0	39.0	17.0	39.0
10	32.68055793390102	32.0	32.0	38.0	27.0	39.0
11	35.56744332927841	37.0	35.0	37.0	32.0	39.0
12	36.55377185823035	37.0	35.0	39.0	33.0	39.0
13	36.7309327735017	39.0	35.0	39.0	33.0	39.0
14	37.86282160481609	40.0	37.0	41.0	33.0	41.0
15	38.20124583295342	40.0	37.0	41.0	33.0	41.0
16	38.304983331813666	40.0	37.0	41.0	34.0	41.0
17	38.27888355398202	40.0	37.0	41.0	34.0	41.0
18	38.237697899964196	40.0	37.0	41.0	34.0	41.0
19	38.21258553704029	40.0	37.0	41.0	34.0	41.0
20	38.177023033009455	40.0	37.0	41.0	34.0	41.0
21	38.092411138218075	40.0	36.0	41.0	34.0	41.0
22	38.05127441016415	40.0	36.0	41.0	33.0	41.0
23	37.96523476678995	40.0	36.0	41.0	33.0	41.0
24	37.86963120942759	40.0	36.0	41.0	33.0	41.0
25	37.764293609157164	40.0	35.0	41.0	33.0	41.0
26	37.71268338896809	40.0	35.0	41.0	33.0	41.0
27	37.65803531120249	40.0	35.0	41.0	33.0	41.0
28	37.59854133910363	40.0	35.0	41.0	33.0	41.0
29	37.599643285245044	40.0	35.0	41.0	33.0	41.0
30	37.5041831699132	40.0	35.0	41.0	33.0	41.0
31	37.441269940110175	40.0	35.0	41.0	33.0	41.0
32	37.302723841844596	40.0	35.0	41.0	33.0	41.0
33	37.172316132891815	40.0	35.0	41.0	32.0	41.0
34	37.00751569522683	40.0	35.0	41.0	32.0	41.0
35	36.87153932201957	40.0	35.0	41.0	31.0	41.0
36	36.761189034357145	40.0	35.0	41.0	31.0	41.0
37	36.69964906740441	40.0	35.0	41.0	31.0	41.0
38	36.59758950671509	39.0	35.0	41.0	31.0	41.0
39	36.53159282923282	39.0	35.0	41.0	31.0	41.0
40	36.41673468116951	39.0	35.0	41.0	30.0	41.0
41	36.35486446396047	39.0	35.0	41.0	30.0	41.0
42	36.32003473743437	39.0	35.0	41.0	30.0	41.0
43	36.220927413884745	39.0	35.0	41.0	30.0	41.0
44	35.97583279774229	39.0	35.0	40.0	29.0	41.0
45	35.91139285547493	38.0	35.0	40.0	29.0	41.0
46	35.914387346856174	38.0	35.0	40.0	29.0	41.0
47	35.86331531226996	38.0	35.0	40.0	29.0	41.0
48	35.79704843003253	38.0	35.0	40.0	29.0	41.0
49	35.699055284115296	38.0	35.0	40.0	29.0	41.0
50	35.58551702512348	38.0	35.0	40.0	28.0	41.0
51	35.19562045905898	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	7.0
15	19.0
16	35.0
17	94.0
18	198.0
19	446.0
20	850.0
21	1437.0
22	2293.0
23	3608.0
24	5383.0
25	7873.0
26	10313.0
27	11676.0
28	12629.0
29	14319.0
30	17627.0
31	22735.0
32	29996.0
33	42227.0
34	65619.0
35	99918.0
36	97428.0
37	163646.0
38	208074.0
39	80846.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.81517994747131	22.537300487702904	29.319551037564022	14.32796852726177
2	32.42623854965652	24.063123389057044	30.48710244874449	13.023535612541949
3	30.332874467096175	24.295188131450722	32.420678781031846	12.951258620421251
4	26.382658859268915	26.903386788655403	31.30027420778857	15.413680144287115
5	23.11373729870858	31.96922556870873	30.375239904016155	14.541797228566535
6	21.273342688570672	40.09004601264514	28.033131773188124	10.603479525596063
7	87.5614632421457	4.110225748845235	6.710863120720369	1.6174478882886811
8	33.48092665775621	63.31186521341727	2.359009827447021	0.8481983013794898
9	85.53681789978627	3.997918422626924	7.584747553145828	2.880516124440965
10	48.8984986400806	21.7950713763096	15.326391776879813	13.980038206729988
11	43.102662239608236	21.99933727557994	19.433615250667728	15.464385234144096
12	37.86469302293516	21.80085353567926	23.73042683455685	16.604026606828732
13	23.83695200140551	32.20217987408236	24.72139999421784	19.23946813029429
14	18.665922398973443	35.22235738637501	28.629250165125132	17.48247004952642
15	19.017188580680024	23.901000535961696	40.05802174536704	17.023789137991233
16	21.39510162145092	19.643663309307723	39.1032982771389	19.85793679210246
17	22.172134884434648	19.881843797188537	27.618261838415332	30.32775947996148
18	25.46996724184326	23.730760420674333	30.294400868213465	20.504871469268934
19	28.991858274826033	25.798883153678677	24.499898812211033	20.709359759284258
20	31.169730840481343	25.011953502543037	23.399398210644065	20.41891744633155
21	26.105337600270428	26.16338158471197	27.591797339761907	20.139483475255695
22	24.28584772016128	22.066721671310926	28.21593696556724	25.431493642960557
23	23.48312832613158	26.36197651998514	26.4836242574929	23.671270896390375
24	23.805261320244895	24.392706473127415	32.674871402551716	19.127160804075977
25	22.717214600397188	24.004745818498016	31.129478115638744	22.148561465466056
26	19.88951627789058	30.426612166108097	26.230654785070463	23.453216770930858
27	20.02194996653019	29.406728209598832	30.054886035862733	20.51643578800825
28	17.860311925258916	27.550766247311852	34.61990085820589	19.969020969223344
29	20.40890986280715	25.9932526647971	32.98944311133548	20.60839436106027
30	22.052488663631774	26.946530593182832	31.420809991571392	19.580170751614002
31	29.399611705759256	24.755314582828323	25.21455147122597	20.63052224018645
32	29.875639095403407	25.749178822174134	25.479863629995176	18.895318452427283
33	27.623043239432548	25.976684554295588	24.87140255171141	21.528869654560456
34	21.635839602899086	27.56099622158124	28.07471884250065	22.72844533301902
35	22.198043406225608	26.074536482089762	28.779475113363684	22.94794499832095
36	28.782699779165988	26.148703795542843	25.81745278088507	19.251143644406092
37	23.4492137375211	29.304873248394898	26.664761519284614	20.581151494799393
38	23.781354315158822	30.43261671622274	23.258958455184928	22.527070513433515
39	22.711432441027533	27.946399382643293	25.632090094938608	23.710078081390566
40	24.206676614945994	24.918326998903613	26.211862767119083	24.66313361903131
41	21.21196284295433	24.181435265389993	26.968547276936523	27.638054614719152
42	24.04633288781054	26.26234546623108	24.115162823383944	25.576158822574442
43	23.02133394416658	25.436386239350263	25.414480750969066	26.12779906551409
44	22.242966336712932	28.0049993439473	27.046828819171857	22.705205500167907
45	21.255329038226744	31.86314518335005	24.251488350060825	22.63003742836238
46	24.699605701209137	28.345590769894518	25.597953115583145	21.3568504133132
47	23.411629701618335	27.63861059158162	25.776199297690027	23.173560409110014
48	23.65826103780865	25.936320634080488	28.35115053851919	22.05426778959167
49	23.188127002906647	24.578069159073877	28.87732704115786	23.35647679686162
50	21.679316993543996	28.172237184177344	27.259879152869175	22.888566669409485
51	20.42203091676137	31.233556984292544	25.476416573447878	22.867995525498213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	601.0
1	1054.5
2	1508.0
3	5786.0
4	10064.0
5	6947.0
6	3830.0
7	3826.0
8	3822.0
9	3957.0
10	4092.0
11	4059.5
12	4027.0
13	3890.5
14	3754.0
15	3464.5
16	3175.0
17	3034.5
18	2894.0
19	2847.0
20	2800.0
21	2983.0
22	3166.0
23	3238.5
24	3311.0
25	3823.5
26	5190.5
27	6045.0
28	6799.5
29	7554.0
30	8798.5
31	10043.0
32	11476.0
33	12909.0
34	15055.5
35	17202.0
36	21330.0
37	25458.0
38	33213.0
39	40968.0
40	50599.5
41	60231.0
42	64630.5
43	69030.0
44	69815.5
45	70601.0
46	72838.0
47	75075.0
48	74382.0
49	73689.0
50	70400.0
51	67111.0
52	63849.0
53	60587.0
54	57140.5
55	53694.0
56	50666.0
57	47638.0
58	44643.0
59	41648.0
60	37377.5
61	33107.0
62	29071.0
63	25035.0
64	21070.5
65	17106.0
66	14592.0
67	12078.0
68	10185.0
69	8292.0
70	7160.0
71	6028.0
72	4762.5
73	3497.0
74	2599.5
75	1272.5
76	843.0
77	621.5
78	400.0
79	298.0
80	196.0
81	144.0
82	92.0
83	64.5
84	37.0
85	31.0
86	25.0
87	17.0
88	9.0
89	6.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	899318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.126279797741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.62880597625815	18.452679467160817
2	6.352149281420279	3.3191605888681126
3	3.0003416721888088	2.3516329804928073
4	2.1235304830533575	2.2191980623713645
5	1.7308406620411392	2.2610213710897034
6	1.434459151603977	2.2486248691941406
7	1.2874893716614246	2.3546115292450884
8	1.148152276310378	2.3997558097038536
9	1.0121780120220933	2.3800001352489413
>10	11.075433060311566	56.87851679492984
>50	0.1766129919646393	2.843357671614352
>100	0.02786369965244439	1.3603049357822279
>500	8.573446046905958E-4	0.14189909053483302
>1k	0.0012860169070358935	0.7892366937639844
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3819	0.42465512755221174	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2158	0.23995961384071038	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1070	0.1189790485679148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0068941130945894555	0.0
2	0.0	0.0	0.0	0.023462223596102824	0.0
3	0.0	0.0	0.0	0.03680566829530822	0.0
4	0.0	0.0	0.0	0.059823110401437535	0.0
5	0.0	0.0	0.0	0.10952744190597764	0.0
6	0.0	0.0	0.0	0.17646705614699137	0.0
7	0.0	0.0	0.0	0.22316911259421027	0.0
8	0.0	0.0	0.0	0.31579485788119444	0.0
9	0.0	0.0	0.0	0.42888055170696016	0.0
10	0.0	0.0	0.0	0.6130200885559947	0.0
11	0.0	0.0	0.0	0.801829831049751	0.0
12	0.0	0.0	0.0	0.9522771700332919	0.0
13	0.0	0.0	0.0	1.0189943935293189	0.0
14	0.0	0.0	0.0	1.0568008201770676	0.0
15	0.0	0.0	0.0	1.0956080051772565	0.0
16	0.0	0.0	0.0	1.1814508327421447	0.0
17	0.0	0.0	0.0	1.2739653826566353	0.0
18	0.0	0.0	0.0	1.4139603566257986	0.0
19	0.0	0.0	0.0	1.4797880171418787	0.0
20	0.0	0.0	0.0	1.5523985953800548	0.0
21	0.0	0.0	0.0	1.6571446362688171	0.0
22	0.0	0.0	0.0	1.7637809984899668	0.0
23	0.0	0.0	0.0	1.8858735174876962	0.0
24	0.0	0.0	0.0	1.972828298777518	0.0
25	0.0	0.0	0.0	2.046884416858108	0.0
26	0.0	0.0	0.0	2.1260555220733934	0.0
27	0.0	0.0	0.0	2.2107863959133476	0.0
28	0.0	0.0	0.0	2.303078555082852	0.0
29	0.0	0.0	0.0	2.4090477450690413	0.0
30	0.0	0.0	0.0	2.5173520378775915	0.0
31	0.0	0.0	0.0	2.622654055628821	0.0
32	0.0	0.0	0.0	2.720283592678007	0.0
33	0.0	0.0	0.0	2.8248072428217825	0.0
34	0.0	0.0	0.0	2.941673579312323	0.0
35	0.0	0.0	0.0	3.078777473596659	0.0
36	0.0	0.0	0.0	3.196755763812133	0.0
37	0.0	0.0	0.0	3.3235184884545843	0.0
38	1.1119537249337831E-4	0.0	0.0	3.4855301461774366	0.0
39	1.1119537249337831E-4	0.0	0.0	3.7284920350754684	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	35	1.2115379E-7	45.000004	4
TCGATAG	30	2.164972E-6	45.000004	1
GCTACGA	30	2.164972E-6	45.000004	2
GCGTAAG	30	2.164972E-6	45.000004	1
TACCCGT	25	3.890127E-5	45.0	30
TTAGCGA	105	0.0	45.0	2
TATGCGA	80	0.0	45.0	2
ACGGGTA	20	7.0324674E-4	45.0	5
TAGCCGT	100	0.0	45.0	44
TCATACG	25	3.890127E-5	45.0	1
GTAACGA	25	3.890127E-5	45.0	2
CGGTCTA	185	0.0	43.783783	31
CGTTTTT	3330	0.0	43.513515	1
TAACGCC	105	0.0	42.857143	12
ATAACGC	105	0.0	42.857143	11
CACGACG	195	0.0	42.692307	26
AGACTTA	740	0.0	41.959457	8
TCACGAC	200	0.0	41.625	25
TAAGCGA	65	0.0	41.538464	2
GAGGGGA	7870	0.0	41.026047	7
>>END_MODULE