FastQCFastQC Report
Thu 26 May 2016
SRR1546918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546918_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences862309
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23310.27032073189541106No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.2299639688325183No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.12872415804543383No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG9920.11503996827123456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC2050.045.00000425
GTCTAAT351.211447E-745.00000433
ATAGACG351.211447E-745.0000041
TCCAATC700.045.00000423
CGGTCTA2050.045.00000431
GTAACGA351.211447E-745.0000042
GCCCGAC351.211447E-745.0000049
AATCGGT700.045.00000426
TCTGCGT207.0323056E-445.016
GTCATCG207.0323056E-445.043
CGTGGTA207.0323056E-445.037
ATCTCGA207.0323056E-445.02
AGATCGA253.8899918E-545.08
TCCGCGA207.0323056E-445.02
CTCGTAG253.8899918E-545.01
AGATACC207.0323056E-445.08
CACGGAC253.8899918E-545.03
CGACGGT2100.045.028
TCACACG207.0323056E-445.045
CGCATCG502.1827873E-1145.021