##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546918_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	862309
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729406743986203	31.0	31.0	34.0	30.0	34.0
2	32.06871782620847	33.0	31.0	34.0	30.0	34.0
3	32.09831162611083	33.0	31.0	34.0	30.0	34.0
4	35.82858464889036	37.0	35.0	37.0	35.0	37.0
5	35.79904303445748	37.0	35.0	37.0	35.0	37.0
6	35.84431798809939	37.0	35.0	37.0	35.0	37.0
7	36.07338088782559	37.0	35.0	37.0	35.0	37.0
8	13.201684083083906	0.0	0.0	35.0	0.0	37.0
9	24.94116030332514	18.0	17.0	39.0	17.0	39.0
10	32.76558171142827	32.0	32.0	38.0	27.0	39.0
11	35.64602132182315	37.0	35.0	37.0	32.0	39.0
12	36.394581292784835	37.0	35.0	39.0	33.0	39.0
13	36.28534898742794	38.0	35.0	39.0	32.0	39.0
14	37.39401884939158	39.0	35.0	41.0	33.0	41.0
15	37.86441867126517	40.0	36.0	41.0	33.0	41.0
16	38.074748147125916	40.0	36.0	41.0	34.0	41.0
17	38.0679373635205	40.0	36.0	41.0	34.0	41.0
18	38.02684652485362	40.0	36.0	41.0	33.0	41.0
19	37.99200286672179	40.0	36.0	41.0	34.0	41.0
20	37.94217734014141	40.0	36.0	41.0	34.0	41.0
21	37.8145827075909	40.0	35.0	41.0	33.0	41.0
22	37.76095344012413	40.0	35.0	41.0	33.0	41.0
23	37.70150607264913	40.0	35.0	41.0	33.0	41.0
24	37.6360051907147	40.0	35.0	41.0	33.0	41.0
25	37.52575584854153	39.0	35.0	41.0	33.0	41.0
26	37.42765644333991	39.0	35.0	41.0	33.0	41.0
27	37.32027614231094	39.0	35.0	41.0	33.0	41.0
28	37.332707880817665	39.0	35.0	41.0	33.0	41.0
29	37.397553545190874	39.0	35.0	41.0	33.0	41.0
30	37.339195114512314	39.0	35.0	41.0	33.0	41.0
31	37.22301054494387	39.0	35.0	41.0	33.0	41.0
32	37.02610549118703	39.0	35.0	41.0	32.0	41.0
33	36.909254107286365	39.0	35.0	41.0	32.0	41.0
34	36.8119873502422	39.0	35.0	41.0	32.0	41.0
35	36.71766617303078	39.0	35.0	41.0	31.0	41.0
36	36.60127750029282	39.0	35.0	41.0	31.0	41.0
37	36.47124522647914	39.0	35.0	41.0	31.0	41.0
38	36.41818420079113	39.0	35.0	41.0	31.0	41.0
39	36.383210658824154	39.0	35.0	41.0	30.0	41.0
40	36.21059620159363	39.0	35.0	41.0	30.0	41.0
41	36.20718211221268	39.0	35.0	41.0	30.0	41.0
42	36.17246022017629	39.0	35.0	41.0	30.0	41.0
43	36.06893120679478	38.0	35.0	41.0	30.0	41.0
44	35.87931008489996	38.0	35.0	40.0	30.0	41.0
45	35.76237404457103	38.0	35.0	40.0	29.0	41.0
46	35.77371916563552	38.0	35.0	40.0	29.0	41.0
47	35.740188261980336	38.0	35.0	40.0	29.0	41.0
48	35.72148730907366	38.0	35.0	40.0	29.0	41.0
49	35.67617872479587	38.0	35.0	40.0	29.0	41.0
50	35.519101621344554	38.0	35.0	40.0	29.0	41.0
51	35.058875646664944	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	3.0
14	9.0
15	8.0
16	40.0
17	101.0
18	242.0
19	460.0
20	933.0
21	1636.0
22	2671.0
23	3817.0
24	5587.0
25	8014.0
26	9992.0
27	11406.0
28	12085.0
29	14041.0
30	17398.0
31	22594.0
32	30015.0
33	42830.0
34	69528.0
35	115534.0
36	84080.0
37	144324.0
38	203906.0
39	61043.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.11863844631101	25.239444329120996	27.109771555208166	14.532145669359823
2	29.056869405282793	26.958897564562122	30.160186197755095	13.824046832399986
3	28.910170252194977	25.14029193711303	32.0839745381296	13.865563272562387
4	26.352154506099325	27.853472479122914	30.815519726687302	14.978853288090463
5	22.409020432350815	31.866767017391677	30.54902592922027	15.175186621037238
6	20.605374639485383	41.30421925319114	27.50788870346941	10.582517403854071
7	86.22871847562764	4.899635745423044	7.029730641800097	1.8419151371492124
8	32.74255516294043	63.88545173481896	2.2312187394541865	1.1407743627864257
9	85.56735462577801	3.952875361384376	7.2555197730743854	3.2242502397632404
10	60.65006859490044	17.685191735213245	11.575896807292978	10.088842862593339
11	55.329701997775736	17.815191538068138	16.074748147125913	10.780358317030206
12	49.36374315935471	19.78501905929313	18.85437818693763	11.99685959441453
13	21.21861188970543	46.122561633938645	20.144171057010883	12.514655419345038
14	12.248741460427759	49.62374276506449	25.280612866153547	12.846902908354199
15	10.252357333624024	22.83404208932065	53.43815268076757	13.475447896287756
16	12.966813520443369	15.55463296799639	53.15461162993776	18.32394188162248
17	13.898730037608328	16.629537671530738	27.78377588544246	41.687956405418475
18	20.372975348743896	22.652784558667484	35.08707435501659	21.88716573757203
19	30.276965681675595	24.23064122025863	23.440205309233697	22.05218778883208
20	33.78162584409996	22.16977904672223	22.59572844537167	21.45286666380613
21	21.559904860090757	29.106503585141752	26.9905567493787	22.343034805388786
22	21.991188773397933	23.468617398171652	24.642210622874167	29.897983205556244
23	18.94170187253061	31.41600052881276	23.058091704945674	26.584205893710955
24	19.803921796015118	22.389769792498974	39.989029454638654	17.817278956847254
25	15.958896404885023	23.45446933755765	38.155232057186	22.431402200371327
26	15.204642419364752	37.402137748765234	26.5560257401929	20.837194091677112
27	16.557753659071167	36.20314759558349	28.223293506156143	19.015805239189202
28	13.111541222462018	28.118110793230734	42.28692962731457	16.48341835699268
29	13.118035414219264	23.209777469561374	40.835245834149944	22.83694128206942
30	17.17446994058974	30.631826874125167	31.894483300069933	20.299219885215162
31	31.07586723552694	25.42997927657023	22.28041224201533	21.213741245887498
32	31.841833959752247	26.73148488534852	24.25789363209708	17.168787522802152
33	28.61549630120989	28.169716424158857	22.75541598197398	20.459371292657274
34	18.34330848918427	28.778083030560968	26.65494619678097	26.223662283473793
35	18.540801499230554	26.515553009420056	31.238222029458118	23.70542346189127
36	31.694207064984823	23.41156128487584	25.370255905945548	19.523975744193788
37	19.544502028855085	32.741395485840926	28.60842227090289	19.1056802144011
38	19.621504588262447	33.5508501012978	22.171982433211294	24.655662877228465
39	19.93055853528144	32.03967487292838	26.527497683544993	21.50226890824519
40	24.124646733363562	25.716651455568712	24.456778254662773	25.701923556404953
41	16.29033211992453	23.688028305398646	26.73681940000626	33.284820174670564
42	23.135674102902787	25.079176953968936	22.66728052241134	29.117868420716935
43	23.320990503404232	26.329424834948956	23.923094853469003	26.426489808177813
44	20.791850717086334	30.801487633783246	26.87563274881742	21.531028900312997
45	16.375220483608544	39.630225360050744	20.332734553390956	23.661819602949755
46	22.849697730164014	33.62159040436781	24.33118522478601	19.19752664068217
47	22.114810352205534	27.437612271239196	25.376866065412745	25.070711311142524
48	23.606271069883302	22.562445712615776	30.5675807628124	23.263702454688516
49	20.994214370950555	21.925087178725956	32.720869201179624	24.359829249143868
50	18.737830638437032	33.432447069437984	26.054001523815707	21.775720768309274
51	17.07334609751261	35.94790266598169	22.41365914074885	24.565092095756857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1040.0
1	1170.0
2	1300.0
3	3356.5
4	5413.0
5	4159.5
6	2906.0
7	3175.5
8	3445.0
9	3812.0
10	4179.0
11	4265.0
12	4351.0
13	4184.0
14	4017.0
15	3864.0
16	3711.0
17	3410.0
18	3109.0
19	2963.0
20	2817.0
21	2798.0
22	2779.0
23	3081.0
24	3383.0
25	3693.0
26	4718.0
27	5433.0
28	6304.0
29	7175.0
30	8347.5
31	9520.0
32	11310.5
33	13101.0
34	14101.0
35	15101.0
36	19371.5
37	23642.0
38	35928.5
39	48215.0
40	64270.0
41	80325.0
42	88448.0
43	96571.0
44	94948.5
45	93326.0
46	89450.5
47	85575.0
48	79241.0
49	72907.0
50	66060.0
51	59213.0
52	52092.5
53	44972.0
54	41058.5
55	37145.0
56	35003.5
57	32862.0
58	30271.5
59	27681.0
60	24962.0
61	22243.0
62	18552.5
63	14862.0
64	12242.5
65	9623.0
66	7804.5
67	5986.0
68	4998.5
69	4011.0
70	3324.0
71	2637.0
72	2168.5
73	1700.0
74	1382.5
75	798.0
76	531.0
77	400.5
78	270.0
79	184.5
80	99.0
81	63.5
82	28.0
83	18.0
84	8.0
85	12.5
86	17.0
87	12.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	862309.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.93550161421064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.17258886143006	17.147549491569237
2	5.807015337992551	2.547595886405631
3	2.0465282095683315	1.3467486853354125
4	1.2367345192466386	1.0851356817313862
5	0.8330822911513849	0.9137038971160749
6	0.6669812200985892	0.8778340578072471
7	0.5682308974570967	0.8725100837890153
8	0.4451475083453508	0.7811627110297028
9	0.44147950894135574	0.8715667032921626
>10	7.988038142763286	44.33969453951425
>50	1.6429282448120228	23.831056377117733
>100	0.14858248956322226	4.536289436013618
>500	0.0010651074520661094	0.2155298210096087
>1k	0.0015976611780991641	0.6336226282688985
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2331	0.27032073189541106	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1983	0.2299639688325183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1110	0.12872415804543383	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	992	0.11503996827123456	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0339785390155965	0.0
2	0.0	0.0	0.0	0.11492400056128371	0.0
3	0.0	0.0	0.0	0.16397834187048957	0.0
4	0.0	0.0	0.0	0.26637782975708246	0.0
5	0.0	0.0	0.0	0.4730322888894816	0.0
6	0.0	0.0	0.0	0.6540578841227449	0.0
7	0.0	0.0	0.0	0.7609801126974205	0.0
8	0.0	0.0	0.0	0.9837540835129867	0.0
9	0.0	0.0	0.0	1.149355973322788	0.0
10	0.0	0.0	0.0	1.5528076362417649	0.0
11	0.0	0.0	0.0	1.8912014138783197	0.0
12	0.0	0.0	0.0	2.2157950340307244	0.0
13	0.0	0.0	0.0	2.3321106471114184	0.0
14	0.0	0.0	0.0	2.374554828953426	0.0
15	0.0	0.0	0.0	2.452833033170244	0.0
16	0.0	0.0	0.0	2.594429607020221	0.0
17	0.0	0.0	0.0	2.735562310030395	0.0
18	0.0	0.0	0.0	2.967961600771881	0.0
19	0.0	0.0	0.0	3.0723325397276384	0.0
20	0.0	0.0	0.0	3.178442994332658	0.0
21	0.0	0.0	0.0	3.337666660095163	0.0
22	0.0	0.0	0.0	3.4847137163128297	0.0
23	0.0	0.0	0.0	3.6631880219271746	0.0
24	0.0	0.0	0.0	3.783678472566099	0.0
25	0.0	0.0	0.0	3.885730057322839	0.0
26	0.0	0.0	0.0	3.9804756763526763	0.0
27	0.0	0.0	0.0	4.075801133932268	0.0
28	1.1596770995084129E-4	0.0	0.0	4.174489655100434	0.0
29	1.1596770995084129E-4	0.0	0.0	4.279092529476093	0.0
30	1.1596770995084129E-4	0.0	0.0	4.411527654239953	0.0
31	1.1596770995084129E-4	0.0	0.0	4.543730843583912	0.0
32	1.1596770995084129E-4	0.0	0.0	4.659582585824803	0.0
33	1.1596770995084129E-4	0.0	0.0	4.765924975849725	0.0
34	1.1596770995084129E-4	0.0	0.0	4.882124621220467	0.0
35	1.1596770995084129E-4	0.0	0.0	5.026040549269461	0.0
36	1.1596770995084129E-4	0.0	0.0	5.152793256245731	0.0
37	1.1596770995084129E-4	0.0	0.0	5.2742114485642615	0.0
38	1.1596770995084129E-4	0.0	0.0	5.39110690019471	0.0
39	1.1596770995084129E-4	0.0	0.0	5.514148640452553	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	205	0.0	45.000004	25
GTCTAAT	35	1.211447E-7	45.000004	33
ATAGACG	35	1.211447E-7	45.000004	1
TCCAATC	70	0.0	45.000004	23
CGGTCTA	205	0.0	45.000004	31
GTAACGA	35	1.211447E-7	45.000004	2
GCCCGAC	35	1.211447E-7	45.000004	9
AATCGGT	70	0.0	45.000004	26
TCTGCGT	20	7.0323056E-4	45.0	16
GTCATCG	20	7.0323056E-4	45.0	43
CGTGGTA	20	7.0323056E-4	45.0	37
ATCTCGA	20	7.0323056E-4	45.0	2
AGATCGA	25	3.8899918E-5	45.0	8
TCCGCGA	20	7.0323056E-4	45.0	2
CTCGTAG	25	3.8899918E-5	45.0	1
AGATACC	20	7.0323056E-4	45.0	8
CACGGAC	25	3.8899918E-5	45.0	3
CGACGGT	210	0.0	45.0	28
TCACACG	20	7.0323056E-4	45.0	45
CGCATCG	50	2.1827873E-11	45.0	21
>>END_MODULE