FastQCFastQC Report
Thu 26 May 2016
SRR1546917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546917_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1043731
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.3363893570278166No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22750.2179680396577279No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.19286578629934342No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13270.12714003895639778No Hit
AAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12140.11631349456900292No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11640.111522988202899No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG11110.10644505145482887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.032987E-445.0000046
GTCGAAA253.890558E-545.00000440
GGTCGAA253.890558E-545.00000439
ACGTTAG253.890558E-545.0000041
ACGTTAC351.2117926E-745.00000427
CGTTGGC253.890558E-545.00000424
ATTACGC253.890558E-545.00000419
CAATTCG302.1653086E-645.00000444
CGATTAC406.8139343E-945.00000410
CCGCTCG502.1827873E-1145.00000419
CGTTCCG207.032987E-445.00000437
TCGGCGC351.2117926E-745.00000418
TTATACG502.1827873E-1145.0000041
GTTACGA302.1653086E-645.0000042
CCGTACA253.890558E-545.00000430
ACTCGTT302.1653086E-645.00000439
CGTACGT351.2117926E-745.00000412
GTATCGA302.1653086E-645.0000042
GCGATAC1250.045.0000049
CGTAAGA600.045.0000042