FastQCFastQC Report
Thu 26 May 2016
SRR1546916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546916_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1257711
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61100.4858031773595047No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.27923744007963675No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16340.12991855839696084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG207.0335396E-445.00000431
CCGTCTA207.0335396E-445.00000430
CGTTAGA1550.045.0000042
TACGATC207.0335396E-445.00000411
TAATGCG406.8157533E-945.0000041
TAGTCGA302.1656651E-645.0000042
TAATACG253.8910162E-545.01
TATAGCG453.8562575E-1045.01
TTACGCG453.8562575E-1045.01
GTAACGA453.8562575E-1045.02
CGTTTTT53700.042.653631
CGGTCTA1800.042.531
GAGCGAT7800.040.9615367
ATATACG556.184564E-1140.9090921
AGGGGAA121200.040.6559378
GAGGGGA119850.040.5882347
GGTACGA501.0822987E-940.52
AATGCGA1750.039.8571432
CGCGACC6050.039.7933910
TAGCGGA403.458572E-739.3750042