FastQCFastQC Report
Thu 26 May 2016
SRR1546915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546915_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2099117
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58280.2776405507649169No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.14944378993643517No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29700.14148806379063197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCGA207.034621E-445.019
CGATACG207.034621E-445.010
ACGTTAG650.044.9999961
CGTTTTT64250.043.3540881
CGCATCG4050.043.33333221
CACGACG14850.043.1818226
TAGTGCG2150.042.9069751
CGGTCTA15150.042.77227431
CGACGGT15100.042.61589428
CGTTAGA2500.042.3000032
AGGGGAA202000.042.1262368
ACGACGG15300.041.91176227
GAGGGGA198650.041.8852277
TCTCACG15450.041.65048623
ATTGCGA2450.041.326532
ACGGTCT15600.041.25000430
TCACGAC15800.040.8702525
TAACGCC4600.040.59782812
GACGGTC15950.040.48589329
CTCACGA15900.040.471724
Produced by FastQC (version 0.11.5)