##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546915_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2099117
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731174107970162	31.0	31.0	33.0	30.0	34.0
2	32.097680119783696	33.0	31.0	34.0	30.0	34.0
3	32.15082818156396	33.0	31.0	34.0	30.0	34.0
4	35.88224858357109	37.0	35.0	37.0	35.0	37.0
5	35.85922366404541	37.0	35.0	37.0	35.0	37.0
6	35.91194297411721	37.0	35.0	37.0	35.0	37.0
7	36.142958682150635	37.0	35.0	37.0	35.0	37.0
8	13.281434050603183	0.0	0.0	37.0	0.0	37.0
9	25.01030052160027	18.0	17.0	39.0	17.0	39.0
10	32.52395554892843	32.0	32.0	37.0	27.0	39.0
11	35.49711664476063	37.0	34.0	37.0	32.0	39.0
12	36.58332432160761	37.0	35.0	39.0	33.0	39.0
13	36.79061576843978	39.0	35.0	39.0	33.0	39.0
14	37.95796851723844	40.0	37.0	41.0	33.0	41.0
15	38.29880135314039	40.0	37.0	41.0	33.0	41.0
16	38.41090372761499	40.0	37.0	41.0	34.0	41.0
17	38.39507087980327	40.0	37.0	41.0	34.0	41.0
18	38.3592758288366	40.0	37.0	41.0	34.0	41.0
19	38.35105189467762	40.0	37.0	41.0	34.0	41.0
20	38.319824954969164	40.0	37.0	41.0	34.0	41.0
21	38.24396496241039	40.0	37.0	41.0	34.0	41.0
22	38.19979639057756	40.0	37.0	41.0	34.0	41.0
23	38.12019101365002	40.0	37.0	41.0	34.0	41.0
24	38.027216682062026	40.0	36.0	41.0	34.0	41.0
25	37.92977856879821	40.0	36.0	41.0	33.0	41.0
26	37.86371984029475	40.0	36.0	41.0	33.0	41.0
27	37.807211794292556	40.0	36.0	41.0	33.0	41.0
28	37.782604304571876	40.0	36.0	41.0	33.0	41.0
29	37.79804365359339	40.0	36.0	41.0	33.0	41.0
30	37.714344174240885	40.0	36.0	41.0	33.0	41.0
31	37.6369020878779	40.0	36.0	41.0	33.0	41.0
32	37.52145545007734	40.0	35.0	41.0	33.0	41.0
33	37.41008385907027	40.0	35.0	41.0	33.0	41.0
34	37.27514569221249	40.0	35.0	41.0	33.0	41.0
35	37.16262885775305	40.0	35.0	41.0	32.0	41.0
36	37.0485485087301	40.0	35.0	41.0	32.0	41.0
37	36.98806307604578	40.0	35.0	41.0	31.0	41.0
38	36.891460552222675	40.0	35.0	41.0	31.0	41.0
39	36.845132024560804	40.0	35.0	41.0	31.0	41.0
40	36.72190306686097	40.0	35.0	41.0	31.0	41.0
41	36.66084644162284	39.0	35.0	41.0	31.0	41.0
42	36.632693175273225	39.0	35.0	41.0	31.0	41.0
43	36.52442574663537	39.0	35.0	41.0	31.0	41.0
44	36.296898648336416	39.0	35.0	41.0	30.0	41.0
45	36.238233504849894	39.0	35.0	40.0	30.0	41.0
46	36.237221174427155	39.0	35.0	40.0	30.0	41.0
47	36.187536950060434	39.0	35.0	40.0	30.0	41.0
48	36.145042891844525	39.0	35.0	40.0	30.0	41.0
49	36.05683580286377	39.0	35.0	40.0	30.0	41.0
50	35.938295483291306	38.0	35.0	40.0	29.0	41.0
51	35.55459748075024	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	2.0
14	10.0
15	28.0
16	71.0
17	190.0
18	456.0
19	936.0
20	1834.0
21	3120.0
22	5067.0
23	7765.0
24	12012.0
25	17347.0
26	21862.0
27	24435.0
28	26512.0
29	30164.0
30	37589.0
31	49171.0
32	66218.0
33	91856.0
34	145382.0
35	226873.0
36	217110.0
37	382615.0
38	519751.0
39	210695.0
40	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.12660371003617	23.93830358193469	30.151249311019825	13.783843397009313
2	30.481578682846166	25.492957276797817	31.02885641915148	12.996607621204534
3	29.882755463368643	25.63053893613362	31.475615699363114	13.011089901134621
4	26.34855513056204	27.214204829935635	31.13318600154255	15.304054037959771
5	22.81959509641435	32.896927612896285	29.968362887823787	14.315114402865586
6	20.428732652824973	42.40154312503782	26.826613285490996	10.34311093664622
7	89.54384152955743	3.7395247620785312	5.483353238528391	1.2332804698356499
8	34.128493075898106	63.33767960528165	1.8431559555756063	0.6906713632446405
9	87.7299359683143	3.616282465436657	6.43341938538919	2.2203621808598566
10	45.85270854364001	23.16092909542441	18.906187697017366	12.08017466391821
11	41.20137181491075	21.976526320352797	20.46269931595047	16.35940254878599
12	36.656079675406374	23.00105234724887	23.68548299118153	16.65738498616323
13	24.131956436920856	35.18770035210043	24.124477101562228	16.555866109416485
14	17.511553667565934	38.4041956689408	28.008443550311867	16.0758071131814
15	16.822216198525382	25.667078109509855	41.348671846304896	16.162033845659867
16	20.19896937617103	20.859294646272694	40.249066631350225	18.69266934620605
17	19.431837291584987	21.36989029196562	27.64066986261366	31.557602553835736
18	22.318813100937206	25.797942658746507	31.803658395411023	20.079585844905264
19	27.77124857737801	26.962956328780148	24.806621069716456	20.45917402412538
20	29.336716343109988	26.076250156613472	23.930681329339908	20.656352170936636
21	24.425460800898662	27.067809941037112	29.221382133535194	19.28534712452903
22	23.305132586701934	22.95617633509709	27.508661975487787	26.230029102713186
23	20.025658407797184	29.398361310970277	26.589227756242266	23.98675252499027
24	20.552546618411455	26.777830868884394	34.32729095138575	18.3423315613184
25	20.64148877837681	26.30687093668433	32.39457352782146	20.6570667571174
26	18.490679652444335	32.8128446389601	25.489336706815298	23.207139001780273
27	18.622020592468168	31.3276010817882	29.722640519799516	20.327737805944118
28	16.533761576891617	28.31409587936261	36.47767132560977	18.674471218136006
29	18.37444030037392	27.08348319793513	34.1635554378341	20.37852106385685
30	20.18944156042755	28.231346799630515	32.20968626331929	19.369525376622647
31	28.164270976796434	26.729620121222396	24.63897915170998	20.467129750271184
32	28.139260460469806	27.678542930193984	25.797132794408316	18.385063814927896
33	26.02051243451413	26.564884187017686	25.88412175214626	21.530481626321926
34	19.797371942583478	28.52513699808062	28.089811096761164	23.58767996257474
35	20.04590501625207	26.20006412219995	29.590870828067228	24.163160033480743
36	27.814885973483133	25.905130585860626	26.752343961770592	19.527639478885643
37	21.00945302238989	30.660177588957644	27.47240863658386	20.857960752068607
38	21.04389607630256	32.488470151973424	23.998471738354745	22.46916203336927
39	20.49890501577568	29.57757952510508	25.819570800484204	24.103944658635037
40	22.996669552006868	25.659551135072512	26.14913794705107	25.19464136586955
41	19.3321286998295	23.98622849512438	28.362497183339467	28.31914562170665
42	22.502414110314003	27.057234065561854	24.421792591837423	26.01855923228672
43	22.493791437066157	26.70232292911734	25.668650199107528	25.135235434708974
44	20.4457874430058	29.783618540557764	27.498467212642268	22.27212680379417
45	19.925139951703503	33.147318610634855	24.12976503929986	22.797776398361787
46	23.42446847888898	29.495354475238873	26.88411365350288	20.19606339236927
47	21.90635395740209	28.43538497377707	26.183819196357327	23.474441872463515
48	22.593452389742925	27.020027945083573	28.315239217251825	22.071280447921673
49	21.836133955372663	25.204073903455598	29.394788380066476	23.565003761105267
50	21.24045491509049	29.831686370983608	27.19152862846616	21.73633008545974
51	19.420737386243836	31.76049739009307	26.264757991098165	22.55400723256493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1442.0
1	2150.0
2	2858.0
3	9425.0
4	15992.0
5	11756.5
6	7521.0
7	7824.0
8	8127.0
9	8771.0
10	9415.0
11	9359.5
12	9304.0
13	8950.5
14	8597.0
15	8308.0
16	8019.0
17	7752.5
18	7486.0
19	7387.5
20	7289.0
21	7864.5
22	8440.0
23	9156.0
24	9872.0
25	11399.0
26	15679.0
27	18432.0
28	22300.5
29	26169.0
30	29739.5
31	33310.0
32	37326.5
33	41343.0
34	47830.0
35	54317.0
36	63915.0
37	73513.0
38	92987.5
39	112462.0
40	137505.0
41	162548.0
42	175376.5
43	188205.0
44	189458.0
45	190711.0
46	192124.5
47	193538.0
48	186248.5
49	178959.0
50	165941.0
51	152923.0
52	140019.0
53	127115.0
54	117291.0
55	107467.0
56	99139.0
57	90811.0
58	80922.5
59	71034.0
60	62366.0
61	53698.0
62	45359.0
63	37020.0
64	31293.0
65	25566.0
66	20648.5
67	15731.0
68	12892.5
69	10054.0
70	8651.0
71	7248.0
72	5867.0
73	4486.0
74	3325.0
75	1748.0
76	1332.0
77	1037.5
78	743.0
79	590.5
80	438.0
81	364.0
82	290.0
83	216.0
84	142.0
85	86.5
86	31.0
87	22.0
88	13.0
89	8.5
90	4.0
91	4.5
92	5.0
93	5.5
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2099117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.912756411848306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85222517890503	15.303396396960512
2	6.129771568897508	2.441212962234586
3	2.317032230981768	1.3841549524182417
4	1.3558106380088548	1.0799170790105186
5	0.9997765468432651	0.9954153421784393
6	0.7535233684934215	0.9002836372466948
7	0.6374133566588149	0.8884859833363897
8	0.5407022269980476	0.8613497390044824
9	0.4826131884699465	0.8649142976832733
>10	7.607972891414182	37.87923439885032
>50	2.0043300761478346	27.298950075027996
>100	0.3105852492327043	8.280422707905936
>500	0.005818926316733735	0.8103718433757651
>1k	0.0021820973687751506	0.7305175074244088
>5k	2.4245526319723894E-4	0.2813730773425058
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5828	0.2776405507649169	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3137	0.14944378993643517	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2970	0.14148806379063197	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01100462718371582	0.0
2	0.0	0.0	0.0	0.028726364466582853	0.0
3	0.0	0.0	0.0	0.04382795241999374	0.0
4	0.0	0.0	0.0	0.06988652847840306	0.0
5	0.0	0.0	0.0	0.126005363207482	0.0
6	0.0	0.0	0.0	0.19350993775001585	0.0
7	0.0	0.0	0.002000841306130149	0.2335267638726188	0.0
8	0.0	0.0	0.002000841306130149	0.32204017212951924	0.0
9	0.0	0.0	0.002000841306130149	0.4348495105322857	0.0
10	0.0	0.0	0.002000841306130149	0.6255963817166933	0.0
11	0.0	0.0	0.002000841306130149	0.8297298340206858	0.0
12	0.0	0.0	0.002000841306130149	1.008900409076769	0.0
13	0.0	0.0	0.002000841306130149	1.0817881995143672	0.0
14	0.0	0.0	0.002000841306130149	1.1109433156894066	0.0
15	0.0	0.0	0.002000841306130149	1.1509601418120095	0.0
16	0.0	0.0	0.002000841306130149	1.2423795338706705	0.0
17	0.0	0.0	0.002000841306130149	1.346185086395851	0.0
18	0.0	0.0	0.002000841306130149	1.50029750604659	0.0
19	0.0	0.0	0.002000841306130149	1.576758227387992	0.0
20	0.0	0.0	0.002000841306130149	1.654552842933481	0.0
21	0.0	0.0	0.002000841306130149	1.7627411907006614	0.0
22	0.0	0.0	0.002000841306130149	1.873740244112167	0.0
23	0.0	0.0	0.002000841306130149	2.00246103480654	0.0
24	0.0	0.0	0.002000841306130149	2.0969293279031134	0.0
25	0.0	0.0	0.002000841306130149	2.179249655926754	0.0
26	0.0	0.0	0.002000841306130149	2.260045533431438	0.0
27	0.0	0.0	0.002000841306130149	2.3438903119740346	0.0
28	0.0	0.0	0.002000841306130149	2.4373105453388257	0.0
29	0.0	0.0	0.002000841306130149	2.5339225969776815	0.0
30	0.0	0.0	0.002000841306130149	2.6490662502376	0.0
31	0.0	0.0	0.002000841306130149	2.762113784033953	0.0
32	0.0	0.0	0.002000841306130149	2.858582918436657	0.0
33	0.0	0.0	0.002000841306130149	2.970344197107641	0.0
34	0.0	0.0	0.002000841306130149	3.0786754621109735	0.0
35	0.0	0.0	0.002000841306130149	3.221973810892866	0.0
36	4.76390787173845E-5	0.0	0.002000841306130149	3.3377844112548276	0.0
37	4.76390787173845E-5	0.0	0.002000841306130149	3.4559769655526584	0.0
38	4.76390787173845E-5	0.0	0.002000841306130149	3.592844038707704	0.0
39	4.76390787173845E-5	0.0	0.002000841306130149	3.7614863773672456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCGA	20	7.034621E-4	45.0	19
CGATACG	20	7.034621E-4	45.0	10
ACGTTAG	65	0.0	44.999996	1
CGTTTTT	6425	0.0	43.354088	1
CGCATCG	405	0.0	43.333332	21
CACGACG	1485	0.0	43.18182	26
TAGTGCG	215	0.0	42.906975	1
CGGTCTA	1515	0.0	42.772274	31
CGACGGT	1510	0.0	42.615894	28
CGTTAGA	250	0.0	42.300003	2
AGGGGAA	20200	0.0	42.126236	8
ACGACGG	1530	0.0	41.911762	27
GAGGGGA	19865	0.0	41.885227	7
TCTCACG	1545	0.0	41.650486	23
ATTGCGA	245	0.0	41.32653	2
ACGGTCT	1560	0.0	41.250004	30
TCACGAC	1580	0.0	40.87025	25
TAACGCC	460	0.0	40.597828	12
GACGGTC	1595	0.0	40.485893	29
CTCACGA	1590	0.0	40.4717	24
>>END_MODULE