FastQCFastQC Report
Thu 26 May 2016
SRR1546914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546914_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1343774
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63250.4706892676893584No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34250.25487916866973165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA207.033712E-445.0000046
TGCGATA207.033712E-445.0000044
ACGTCGA207.033712E-445.0000042
CGAAGTA207.033712E-445.00000435
GCGTATG551.8189894E-1245.01
CGTTTTT63100.043.003171
CGTAGGA900.042.52
CGTATGG650.041.538462
GTTACGA650.041.538462
GAGCGAT9500.040.2631577
CGTTTGA1350.040.0000042
CCGCTCG451.9286745E-840.019
GCGCGAC3900.039.8076939
CGGTCTA1250.039.60000231
GTTTTTA46800.039.5673072
CGCATCG403.4588084E-739.37500421
TTTAGCG1200.039.3749961
GAGGGGA89850.038.7145237
ACCCGTA356.24908E-638.57142633
TTAGCGA2700.038.3333362