##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546914_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1343774
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74937005776269	31.0	31.0	33.0	30.0	34.0
2	32.12302068651425	33.0	31.0	34.0	30.0	34.0
3	32.14442383912771	33.0	31.0	34.0	30.0	34.0
4	35.893630923057	37.0	35.0	37.0	35.0	37.0
5	35.88610212729224	37.0	35.0	37.0	35.0	37.0
6	35.92810547011626	37.0	35.0	37.0	35.0	37.0
7	36.16671032480164	37.0	35.0	37.0	35.0	37.0
8	13.262494288474103	0.0	0.0	37.0	0.0	37.0
9	25.002140985016826	18.0	17.0	39.0	17.0	39.0
10	32.549992781524274	32.0	32.0	37.0	27.0	39.0
11	35.49752041637954	37.0	34.0	37.0	32.0	39.0
12	36.66257049176424	37.0	35.0	39.0	33.0	39.0
13	36.95994936648573	39.0	37.0	39.0	33.0	39.0
14	38.13515888832497	40.0	37.0	41.0	33.0	41.0
15	38.407030497687856	40.0	38.0	41.0	34.0	41.0
16	38.490572075363865	40.0	38.0	41.0	34.0	41.0
17	38.456590914841335	40.0	37.0	41.0	34.0	41.0
18	38.39170053892991	40.0	37.0	41.0	34.0	41.0
19	38.379830239311076	40.0	37.0	41.0	34.0	41.0
20	38.35459682952639	40.0	37.0	41.0	34.0	41.0
21	38.29859931804009	40.0	37.0	41.0	34.0	41.0
22	38.23914958914222	40.0	37.0	41.0	34.0	41.0
23	38.13907770205407	40.0	37.0	41.0	34.0	41.0
24	38.042394777693275	40.0	36.0	41.0	34.0	41.0
25	37.93972275099831	40.0	36.0	41.0	33.0	41.0
26	37.88684630004748	40.0	36.0	41.0	33.0	41.0
27	37.83556609965664	40.0	36.0	41.0	33.0	41.0
28	37.77919352510169	40.0	36.0	41.0	33.0	41.0
29	37.79919614458979	40.0	36.0	41.0	33.0	41.0
30	37.686557412183895	40.0	36.0	41.0	33.0	41.0
31	37.60882335868978	40.0	36.0	41.0	33.0	41.0
32	37.463918783962185	40.0	36.0	41.0	33.0	41.0
33	37.30970460806653	40.0	36.0	41.0	33.0	41.0
34	37.11871490295243	40.0	35.0	41.0	32.0	41.0
35	36.95623222357331	40.0	35.0	41.0	31.0	41.0
36	36.83468871997821	40.0	35.0	41.0	31.0	41.0
37	36.7855859690692	40.0	35.0	41.0	31.0	41.0
38	36.693881560440964	40.0	35.0	41.0	31.0	41.0
39	36.61331072040388	40.0	35.0	41.0	31.0	41.0
40	36.50384141976255	40.0	35.0	41.0	30.0	41.0
41	36.43894881133286	39.0	35.0	41.0	30.0	41.0
42	36.39810414548875	39.0	35.0	41.0	30.0	41.0
43	36.302611153363586	39.0	35.0	41.0	30.0	41.0
44	36.13035897405367	39.0	35.0	41.0	30.0	41.0
45	36.03040020122431	39.0	35.0	40.0	29.0	41.0
46	36.00404829978851	39.0	35.0	40.0	29.0	41.0
47	35.95679630652178	39.0	35.0	40.0	29.0	41.0
48	35.8836753799374	39.0	35.0	40.0	29.0	41.0
49	35.78510002426003	39.0	35.0	40.0	28.0	41.0
50	35.677061023654275	38.0	35.0	40.0	28.0	41.0
51	35.32364817298147	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	4.0
14	15.0
15	22.0
16	53.0
17	87.0
18	261.0
19	597.0
20	1136.0
21	2070.0
22	3250.0
23	5301.0
24	8312.0
25	12731.0
26	16432.0
27	18068.0
28	18654.0
29	20180.0
30	24162.0
31	31354.0
32	41773.0
33	58335.0
34	90426.0
35	135983.0
36	142822.0
37	253057.0
38	321085.0
39	137562.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.22550815836592	22.73157539883939	29.58667156828455	15.456244874510148
2	32.4256906295255	24.71137259688013	30.543826566074355	12.319110207520016
3	29.03672790216212	23.95573958120934	33.749499543822104	13.258032972806438
4	25.8085064899306	26.351008428500627	33.38001777084539	14.460467310723379
5	23.831090644706624	31.212168117555482	31.225563227149806	13.73117801058809
6	21.44802623060128	40.116046299452144	27.90119469494126	10.53473277500532
7	87.61465841726361	3.5658525912839507	7.38003563099152	1.4394533604609108
8	33.327628008876495	63.384467923921726	2.5768469995698684	0.7110570676319083
9	85.50678908804605	3.5508203016281015	8.621166952180948	2.321223658144896
10	43.72372139958058	22.570089910952287	20.682718969112365	13.023469720354763
11	38.06242716409158	22.997542741562196	22.42936684293639	16.510663251409834
12	34.007057734410694	22.62820980313654	25.66465789634269	17.700074566110075
13	23.96280922238412	29.615173384810245	27.247587764013893	19.17442962879175
14	19.400137225456067	31.798501831409148	30.056914332320762	18.74444661081402
15	19.145258056786336	24.57585873815091	39.49235511328542	16.786528091777335
16	22.506314305828212	21.871907031986034	37.06099388736499	18.560784774820764
17	21.93791515537583	21.900111179409635	29.46522257462936	26.696751090585174
18	24.07138402737365	24.33697928371884	32.578469296176294	19.013167392731216
19	27.566912293287416	25.272776523433254	27.90238537134965	19.257925811929685
20	28.56135034611475	25.706033901534038	26.73626666388842	18.99634908846279
21	25.673513552130046	26.08757127314563	29.63422420734439	18.60469096737993
22	23.96965561173233	22.313573562221027	30.178735412353564	23.538035413693077
23	22.322578052559432	26.008688961090183	29.750836078090515	21.91789690825987
24	22.09158682933291	24.61336504501501	34.097400306896844	19.197647818755236
25	21.226560418641824	25.423620340920422	32.15719309943488	21.192626141002876
26	20.24737790729691	30.32466768965615	28.088651811986242	21.3393025910607
27	20.15123078731989	28.940134278531954	30.690503016132176	20.218131918015974
28	17.89132696420678	27.443305198642033	34.84201956579008	19.82334827136111
29	19.652039703104837	25.93695070748504	34.01338320283024	20.397626386579887
30	21.806419829524906	26.13073329294956	32.38572855256911	19.677118324956428
31	27.244387821166356	24.962754153600233	27.9602075944318	19.832650430801607
32	27.275568659610915	25.48836337062631	28.616791216380133	18.61927675338264
33	25.968057128654078	25.50525609217026	27.915631646392917	20.611055132782745
34	21.6226091589806	26.671970137835675	30.2758499569124	21.429570746271324
35	22.085707864566512	26.018884127836973	30.74683689370385	21.148571113892665
36	26.611543310110182	26.281279441334632	28.179589722676578	18.927587525878607
37	22.534816122353906	29.138902821456586	28.115069944797266	20.211211111392245
38	22.710589727141617	29.65148901526596	26.22025727540494	21.41766398218748
39	22.529160409414082	28.176389779829048	27.53096874920932	21.76348106154755
40	24.153689534103204	25.339379985027243	27.957528572512935	22.549401908356614
41	20.714643980312168	25.114416561118162	28.587024306170534	25.58391515239914
42	22.61898206097156	26.363733782615235	26.690202370339062	24.327081786074146
43	22.397962752665254	26.14055637331873	27.257931765311728	24.20354910870429
44	21.922436362066836	27.385557392835402	28.30632234289397	22.385683902203795
45	21.416622140330144	30.39863846152701	26.271158691863366	21.91358070627948
46	23.57822074247604	27.725346672878025	27.573758682635624	21.12267390201031
47	22.413739215076344	27.336739660091652	28.265392841355762	21.984128283476238
48	22.965394478535824	25.621570293814287	29.934051410430623	21.478983817219262
49	22.69927830126197	24.917508450081634	30.30494711164228	22.07826613701411
50	20.99594128179292	28.749551635914965	28.659060228877774	21.595446853414337
51	20.664412319333458	28.946087660573877	27.6731801627357	22.716319857356968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	851.0
1	1511.5
2	2172.0
3	9970.5
4	17769.0
5	12477.5
6	7186.0
7	7125.0
8	7064.0
9	7245.0
10	7426.0
11	7308.0
12	7190.0
13	6838.5
14	6487.0
15	6161.0
16	5835.0
17	5672.0
18	5509.0
19	5403.5
20	5298.0
21	5485.0
22	5672.0
23	6287.0
24	6902.0
25	8017.5
26	11161.5
27	13190.0
28	15156.0
29	17122.0
30	20126.0
31	23130.0
32	25666.0
33	28202.0
34	32051.0
35	35900.0
36	42086.5
37	48273.0
38	58172.0
39	68071.0
40	78882.0
41	89693.0
42	97111.5
43	104530.0
44	106430.0
45	108330.0
46	108855.5
47	109381.0
48	106541.0
49	103701.0
50	100213.0
51	96725.0
52	90683.5
53	84642.0
54	78248.0
55	71854.0
56	66909.5
57	61965.0
58	58198.5
59	54432.0
60	48735.5
61	43039.0
62	37157.0
63	31275.0
64	25884.5
65	20494.0
66	16720.0
67	12946.0
68	10812.5
69	8679.0
70	7323.5
71	5968.0
72	4774.5
73	3581.0
74	2934.0
75	1705.0
76	1123.0
77	772.5
78	422.0
79	307.5
80	193.0
81	137.0
82	81.0
83	53.5
84	26.0
85	20.5
86	15.0
87	8.5
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1343774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.076017642564867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.9539596036163	18.04318760472682
2	5.875104705087572	2.946484584733837
3	2.8453875990487845	2.140529689010479
4	1.8903269263084725	1.8960748541730674
5	1.5161451177617775	1.9009440860841462
6	1.333251707297535	2.0059586000503633
7	1.1475435745488956	2.0143076044700043
8	1.0330980349948342	2.0724787641623648
9	0.9302619975515977	2.099453963653035
>10	11.125927155627483	57.99190594060343
>50	0.3231108127440438	4.927240697054888
>100	0.024395250162923585	1.0852340926654014
>500	8.925091498604581E-4	0.14883082500054196
>1k	2.97503049953486E-4	0.2555115667302537
>5k	2.97503049953486E-4	0.47185712688141745
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6325	0.4706892676893584	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3425	0.25487916866973165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.441727552400925E-5	0.0	0.0	0.011683512257269452	0.0
2	7.441727552400925E-5	0.0	0.0	0.03490170222076033	0.0
3	7.441727552400925E-5	0.0	0.0	0.05447344568357477	0.0
4	7.441727552400925E-5	0.0	0.0	0.07999857118830994	0.0
5	7.441727552400925E-5	0.0	0.0	0.13871380157675323	0.0
6	7.441727552400925E-5	0.0	0.0	0.2179682000098231	0.0
7	7.441727552400925E-5	0.0	0.0	0.265520839069665	0.0
8	7.441727552400925E-5	0.0	0.0	0.36330513910821316	0.0
9	7.441727552400925E-5	0.0	0.0	0.49762832142904984	0.0
10	7.441727552400925E-5	0.0	0.0	0.7067408656515158	0.0
11	7.441727552400925E-5	0.0	0.0	0.9187556836194182	0.0
12	7.441727552400925E-5	0.0	0.0	1.0794969987512781	0.0
13	7.441727552400925E-5	0.0	0.0	1.1539886915508113	0.0
14	7.441727552400925E-5	0.0	0.0	1.1881462210163316	0.0
15	7.441727552400925E-5	0.0	0.0	1.2271408733909124	0.0
16	7.441727552400925E-5	0.0	0.0	1.3129439920700952	0.0
17	7.441727552400925E-5	0.0	0.0	1.4166816741505641	0.0
18	7.441727552400925E-5	0.0	0.0	1.5520466983287369	0.0
19	7.441727552400925E-5	0.0	0.0	1.6293662475981825	0.0
20	7.441727552400925E-5	0.0	0.0	1.7103322433683046	0.0
21	7.441727552400925E-5	0.0	0.0	1.8224790775829864	0.0
22	7.441727552400925E-5	0.0	0.0	1.9355189191039566	0.0
23	7.441727552400925E-5	0.0	0.0	2.058381840994096	0.0
24	7.441727552400925E-5	0.0	0.0	2.159514918431224	0.0
25	7.441727552400925E-5	0.0	0.0	2.2497086563663236	0.0
26	7.441727552400925E-5	0.0	0.0	2.334172264086074	0.0
27	7.441727552400925E-5	0.0	0.0	2.422431152857549	0.0
28	7.441727552400925E-5	0.0	0.0	2.5152294954359884	0.0
29	7.441727552400925E-5	0.0	0.0	2.6195625157206495	0.0
30	7.441727552400925E-5	0.0	0.0	2.737290645599632	0.0
31	7.441727552400925E-5	0.0	0.0	2.8472049615485937	0.0
32	7.441727552400925E-5	0.0	0.0	2.9503473054248706	0.0
33	7.441727552400925E-5	0.0	0.0	3.0600383695472604	0.0
34	7.441727552400925E-5	0.0	0.0	3.1750874775073785	0.0
35	7.441727552400925E-5	0.0	0.0	3.307178141562495	0.0
36	7.441727552400925E-5	0.0	0.0	3.422822587726805	0.0
37	7.441727552400925E-5	0.0	0.0	3.540922803983408	0.0
38	7.441727552400925E-5	0.0	0.0	3.686631829459418	0.0
39	7.441727552400925E-5	0.0	0.0	3.885251537832999	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.033712E-4	45.000004	6
TGCGATA	20	7.033712E-4	45.000004	4
ACGTCGA	20	7.033712E-4	45.000004	2
CGAAGTA	20	7.033712E-4	45.000004	35
GCGTATG	55	1.8189894E-12	45.0	1
CGTTTTT	6310	0.0	43.00317	1
CGTAGGA	90	0.0	42.5	2
CGTATGG	65	0.0	41.53846	2
GTTACGA	65	0.0	41.53846	2
GAGCGAT	950	0.0	40.263157	7
CGTTTGA	135	0.0	40.000004	2
CCGCTCG	45	1.9286745E-8	40.0	19
GCGCGAC	390	0.0	39.807693	9
CGGTCTA	125	0.0	39.600002	31
GTTTTTA	4680	0.0	39.567307	2
CGCATCG	40	3.4588084E-7	39.375004	21
TTTAGCG	120	0.0	39.374996	1
GAGGGGA	8985	0.0	38.714523	7
ACCCGTA	35	6.24908E-6	38.571426	33
TTAGCGA	270	0.0	38.333336	2
>>END_MODULE