FastQCFastQC Report
Thu 26 May 2016
SRR1546913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546913_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences842648
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85081.0096742649362487No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.5581215406670401No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13240.1571237337535958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC302.1648084E-645.00000444
ACACGTG302.1648084E-645.00000442
GCGACAC302.1648084E-645.00000432
GTCGTTT302.1648084E-645.0000049
GTCGATG253.889916E-545.01
ACGCATT207.032215E-445.039
CGGCAAC253.889916E-545.036
GCACGGT207.032215E-445.031
GCGCCTA207.032215E-445.035
CGCCTAG207.032215E-445.036
TGCGCGT351.2114106E-745.037
TCTACGA351.2114106E-745.02
TGCGATT207.032215E-445.039
TAATTCG207.032215E-445.038
TATTACG207.032215E-445.01
ACTCGCG207.032215E-445.011
TCATCGG207.032215E-445.040
CGTAAGA900.045.02
TGTACGT207.032215E-445.015
CGATCTA207.032215E-445.010