FastQCFastQC Report
Thu 26 May 2016
SRR1546911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546911_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences805645
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.6115596819939303No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27160.33712118861285056No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8530.10587789907465447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTAT207.0320297E-445.035
ATTCGCG351.2113196E-745.01
GCGTTGA207.0320297E-445.02
CCGAACT207.0320297E-445.012
ATCGGTA207.0320297E-445.045
ATACCGG207.0320297E-445.012
TAGACGA600.044.9999962
CGTTTTT39900.043.1390951
CGTTGAG650.041.538463
TGCGTAG603.6379788E-1241.2499961
CGGTCTA1050.040.71428731
GAGGGGA67450.040.530027
TTCGCGA501.0804797E-940.52
TAACGCC451.9270374E-840.012
AGGGGAA68850.040.08
TACGTAG451.9270374E-840.01
GTTTTTA29750.039.4789922
AACGCGA403.4564982E-739.3752
CGGTAGG3150.039.28571331
GAGCGAT4650.039.193557