FastQCFastQC Report
Thu 26 May 2016
SRR1546910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546910_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences635899
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.2583743644824099No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13340.20978174206910216No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.15081011292673838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC850.045.00000425
TTAGCGA650.045.0000042
CACGACG850.045.00000426
TGTGCGA900.045.0000042
ATGTACG453.8380676E-1045.0000041
CTTGCGA453.8380676E-1045.0000042
TCGTAGA650.045.0000042
CGTAAGA551.8189894E-1245.0000042
CGGTCTA850.045.00000431
GCTAATG551.8189894E-1245.0000041
ACGAGAT551.8189894E-1245.0000045
AACCGTG253.8888327E-545.029
AGCCGTT406.8066583E-945.045
ATCTCGT253.8888327E-545.019
CTCACGT800.045.045
GGTACGA207.030906E-445.02
CTACTTA207.030906E-445.021
CTCCGAT253.8888327E-545.028
TCCGATA253.8888327E-545.029
CGTGCCG253.8888327E-545.041