##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546910_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	635899
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.808842284702443	31.0	31.0	34.0	30.0	34.0
2	32.144839038903974	33.0	31.0	34.0	30.0	34.0
3	32.17690230681288	33.0	31.0	34.0	30.0	34.0
4	35.888663136755994	37.0	35.0	37.0	35.0	37.0
5	35.85737043146789	37.0	35.0	37.0	35.0	37.0
6	35.89808129907422	37.0	35.0	37.0	35.0	37.0
7	36.1326877381471	37.0	35.0	37.0	35.0	37.0
8	13.313401971067732	0.0	0.0	37.0	0.0	37.0
9	25.02825920468502	18.0	17.0	39.0	17.0	39.0
10	32.90119028336261	32.0	32.0	38.0	27.0	39.0
11	35.718570087388095	37.0	35.0	37.0	32.0	39.0
12	36.43996452266791	37.0	35.0	39.0	33.0	39.0
13	36.34180428023947	38.0	35.0	39.0	32.0	39.0
14	37.47793753410526	39.0	35.0	41.0	33.0	41.0
15	37.94583416548854	40.0	36.0	41.0	33.0	41.0
16	38.14261698791789	40.0	36.0	41.0	34.0	41.0
17	38.15748570134566	40.0	36.0	41.0	34.0	41.0
18	38.12450090344536	40.0	36.0	41.0	34.0	41.0
19	38.060893317964016	40.0	36.0	41.0	34.0	41.0
20	38.03767736700325	40.0	36.0	41.0	34.0	41.0
21	37.91965390730289	40.0	35.0	41.0	34.0	41.0
22	37.86088828571833	40.0	35.0	41.0	33.0	41.0
23	37.791013981780125	40.0	35.0	41.0	33.0	41.0
24	37.694368130788064	40.0	35.0	41.0	33.0	41.0
25	37.56051511324912	40.0	35.0	41.0	33.0	41.0
26	37.485858603331664	39.0	35.0	41.0	33.0	41.0
27	37.386081751976334	39.0	35.0	41.0	33.0	41.0
28	37.40974431474181	39.0	35.0	41.0	33.0	41.0
29	37.46586486218723	40.0	35.0	41.0	33.0	41.0
30	37.418203205225986	40.0	35.0	41.0	33.0	41.0
31	37.285205669453795	39.0	35.0	41.0	33.0	41.0
32	37.105818691333056	39.0	35.0	41.0	33.0	41.0
33	36.97845412557655	39.0	35.0	41.0	32.0	41.0
34	36.88121069540917	40.0	35.0	41.0	32.0	41.0
35	36.802608590357906	40.0	35.0	41.0	32.0	41.0
36	36.65578181440763	39.0	35.0	41.0	31.0	41.0
37	36.535967189758125	39.0	35.0	41.0	31.0	41.0
38	36.48262695805466	39.0	35.0	41.0	31.0	41.0
39	36.47417750303114	39.0	35.0	41.0	31.0	41.0
40	36.3461068503017	39.0	35.0	41.0	30.0	41.0
41	36.34017823585192	39.0	35.0	41.0	31.0	41.0
42	36.308031621373836	39.0	35.0	41.0	31.0	41.0
43	36.19245351856191	39.0	35.0	41.0	30.0	41.0
44	36.008364535877554	38.0	35.0	40.0	30.0	41.0
45	35.87562175754326	38.0	35.0	40.0	29.0	41.0
46	35.90977026225863	38.0	35.0	40.0	30.0	41.0
47	35.86346416647927	38.0	35.0	40.0	30.0	41.0
48	35.83969938622328	38.0	35.0	40.0	30.0	41.0
49	35.79368421714769	38.0	35.0	40.0	29.0	41.0
50	35.646835425122546	38.0	35.0	40.0	29.0	41.0
51	35.17943730057761	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	3.0
14	2.0
15	13.0
16	31.0
17	59.0
18	138.0
19	330.0
20	613.0
21	1068.0
22	1701.0
23	2720.0
24	4042.0
25	5709.0
26	7346.0
27	8112.0
28	8756.0
29	10017.0
30	12087.0
31	15475.0
32	21330.0
33	30274.0
34	50916.0
35	85183.0
36	60279.0
37	106031.0
38	156527.0
39	47127.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41253721109799	23.073632762435544	30.40986068542331	14.103969341043154
2	29.538967666248883	26.115153507082102	31.026625297413585	13.319253529255432
3	29.318649659773016	24.31596841636801	31.96419557193831	14.401186351920666
4	25.915436256386627	27.772177657143665	31.435495259467306	14.8768908270024
5	23.709582811106795	31.974260063311945	30.310473833108713	14.005683292472545
6	21.165468101066367	39.48960448121478	28.22885395322213	11.11607346449672
7	87.26212810524942	4.625577332249304	6.480431640873786	1.6318629216274911
8	33.688211492705605	63.39623116249593	2.1064665929652353	0.8090907518332314
9	86.79727440992988	4.273949164883103	6.616931305128644	2.311845120058374
10	62.442935120199905	17.904887411365642	10.961331909627157	8.690845558807295
11	57.01833152749101	17.258243840609907	16.077238680985502	9.646185950913589
12	48.98450854616849	20.998774962690618	18.51158753198228	11.50512895915861
13	21.766349687607622	46.68005453696263	19.49318995626664	12.060405819163107
14	12.903149714026913	48.257191786746006	25.50200582167923	13.337652677547847
15	11.628418978485577	22.353235340832427	53.68352521390975	12.33482046677224
16	14.005368777117122	15.941997078152347	52.05968243384562	17.992951710884906
17	14.653270409294558	15.629054299503537	28.008693204423974	41.70898208677793
18	21.97109918398991	21.797014934761652	34.76794270788285	21.463943173365582
19	30.190486225013718	24.140468848040335	23.95443301530589	21.714611911640056
20	34.12444429068138	23.064197301772765	22.429348056845505	20.382010350700348
21	22.033687739719674	28.911981305207274	27.08732047070368	21.96701048436937
22	23.01670548310345	24.091247194916175	24.86133804267659	28.03070927930379
23	18.950021937446042	31.221467560099953	23.620732223198967	26.207778279255038
24	20.569776017889634	22.96339513035875	40.061550655056855	16.40527819669476
25	15.46456276861577	24.745596391879843	38.16958353449211	21.620257305012274
26	14.73976213203669	37.74467328931167	26.389725412368946	21.125839166282695
27	16.64037842487565	35.453114409678264	29.78224529367085	18.124261871775236
28	13.114975805906285	29.242222428404514	42.37654092866949	15.266260837019715
29	14.175364326724841	24.107130220365182	41.21456394804836	20.50294150486162
30	17.76823048943307	30.188913648236593	32.42385976389332	19.618996098437016
31	31.210774038015472	25.761638247583342	23.956477365116157	19.07111034928503
32	32.80410882860328	27.087005955348253	24.83271714533283	15.27616807071563
33	28.989194824964343	28.035269751957465	23.745123046269924	19.230412376808268
34	18.874066479110677	29.516008045302794	27.513488777305827	24.0964366982807
35	19.386569250777246	25.358429561927288	31.63599879855134	23.619002388744125
36	31.887611082892093	23.551853360360685	26.046589159599243	18.513946397147972
37	20.323667752268832	31.698587354281106	29.34648426872821	18.63126062472185
38	19.725774061604124	33.946743114865725	22.86275021662245	23.464732606907702
39	20.37729262036896	32.10242507064801	26.57434592600397	20.945936382979056
40	23.812429332331078	25.862440418997355	24.340500614091233	25.984629634580337
41	16.25132293021376	22.694169986114147	27.99642710556236	33.05807997810973
42	22.739460197295482	25.84372675534951	23.12474150769226	28.292071539662743
43	23.288604007869175	26.350882765973843	24.008215141083724	26.352298085073254
44	20.041861993807192	31.746236430628134	26.98463120715711	21.22727036840756
45	16.167347330315035	40.197421288600864	19.990438733195052	23.64479264788905
46	22.781290739567133	33.44257500011794	25.020954585555256	18.75517967475967
47	22.33735231538342	27.419291428355763	25.929274932025372	24.314081324235453
48	24.180255040501713	22.53439618555777	30.78602105051274	22.49932772342778
49	20.53958254376874	22.04846996142469	32.39193645531759	25.020011039488978
50	18.83506657503786	33.43707098139799	26.370068202654824	21.357794240909328
51	17.435315985714713	36.45594662045388	22.752669842223373	23.35606755160804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	697.0
1	852.0
2	1007.0
3	2760.0
4	4513.0
5	3414.5
6	2316.0
7	2504.5
8	2693.0
9	2917.0
10	3141.0
11	3092.0
12	3043.0
13	2964.0
14	2885.0
15	2743.5
16	2602.0
17	2401.5
18	2201.0
19	2049.0
20	1897.0
21	2030.5
22	2164.0
23	2243.0
24	2322.0
25	2447.0
26	3410.5
27	4249.0
28	4752.0
29	5255.0
30	6245.5
31	7236.0
32	8442.5
33	9649.0
34	11223.0
35	12797.0
36	15727.0
37	18657.0
38	27605.5
39	36554.0
40	49214.0
41	61874.0
42	68007.0
43	74140.0
44	71699.0
45	69258.0
46	65009.5
47	60761.0
48	56506.0
49	52251.0
50	47936.0
51	43621.0
52	38906.0
53	34191.0
54	30132.0
55	26073.0
56	24099.5
57	22126.0
58	20446.5
59	18767.0
60	16750.0
61	14733.0
62	12795.5
63	10858.0
64	8924.5
65	6991.0
66	5610.0
67	4229.0
68	3400.0
69	2571.0
70	2301.0
71	2031.0
72	1698.5
73	1366.0
74	1108.5
75	617.5
76	384.0
77	307.5
78	231.0
79	168.0
80	105.0
81	62.5
82	20.0
83	14.5
84	9.0
85	6.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	635899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.182064130959247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64047710113725	17.222260422156996
2	4.8980991623009285	2.1729989947591397
3	1.7120927407109534	1.1393325291780045
4	1.1112677693662305	0.9860085170699904
5	0.843318827838985	0.9353276160984876
6	0.6822761762662414	0.9080576338178054
7	0.583672348651272	0.9062940228475086
8	0.5406351887261588	0.9593923542221529
9	0.47359136196401186	0.9454710566658642
>10	10.346024839491298	56.23224821951317
>50	1.1144254215420288	15.476617888297945
>100	0.051982783241257574	1.4942723537793279
>500	7.120929211131174E-4	0.1514806751875661
>1k	0.0014241858422262348	0.4702377164060316
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1643	0.2583743644824099	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1334	0.20978174206910216	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.15081011292673838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009592718340491179	0.0
2	0.0	0.0	0.0	0.036326523551696105	0.0
3	0.0	0.0	0.0	0.0542538988109747	0.0
4	0.0	0.0	0.0	0.08947961861868	0.0
5	0.0	0.0	0.0	0.16197540804435925	0.0
6	0.0	0.0	0.0	0.24563649258765935	0.0
7	0.0	0.0	0.0	0.29910410301006923	0.0
8	0.0	0.0	0.0	0.4219223493038989	0.0
9	0.0	0.0	0.0	0.5430107611428859	0.0
10	0.0	0.0	0.0	0.742728011838358	0.0
11	0.0	0.0	0.0	0.9424452625338301	0.0
12	0.0	0.0	0.0	1.1067795357438839	0.0
13	0.0	0.0	0.0	1.1885535281546282	0.0
14	0.0	0.0	0.0	1.216859910142963	0.0
15	0.0	0.0	0.0	1.2613638329357335	0.0
16	0.0	0.0	0.0	1.3549321511749508	0.0
17	0.0	0.0	0.0	1.458722218465511	0.0
18	0.0	0.0	0.0	1.6206976265098703	0.0
19	0.0	0.0	0.0	1.68580230508304	0.0
20	0.0	0.0	0.0	1.7553101986321726	0.0
21	0.0	0.0	0.0	1.8616163887661406	0.0
22	0.0	0.0	0.0	1.9580153452041913	0.0
23	0.0	0.0	0.0	2.0896400214499473	0.0
24	0.0	0.0	0.0	2.17927689774634	0.0
25	0.0	0.0	0.0	2.2476839875514822	0.0
26	0.0	0.0	0.0	2.327413630151958	0.0
27	0.0	0.0	0.0	2.3940908855022576	0.0
28	0.0	0.0	0.0	2.4689455400936313	0.0
29	0.0	0.0	0.0	2.5455300291398477	0.0
30	0.0	0.0	0.0	2.660170876192603	0.0
31	0.0	0.0	0.0	2.7718238273688116	0.0
32	0.0	0.0	0.0	2.8597308692103622	0.0
33	0.0	0.0	0.0	2.954871764226709	0.0
34	0.0	0.0	0.0	3.0556739356407228	0.0
35	0.0	0.0	0.0	3.1954760111275533	0.0
36	0.0	0.0	0.0	3.3035120357163636	0.0
37	0.0	0.0	0.0	3.4000682498321275	0.0
38	0.0	0.0	0.0	3.493007537360493	0.0
39	0.0	0.0	0.0	3.5887774630876916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	85	0.0	45.000004	25
TTAGCGA	65	0.0	45.000004	2
CACGACG	85	0.0	45.000004	26
TGTGCGA	90	0.0	45.000004	2
ATGTACG	45	3.8380676E-10	45.000004	1
CTTGCGA	45	3.8380676E-10	45.000004	2
TCGTAGA	65	0.0	45.000004	2
CGTAAGA	55	1.8189894E-12	45.000004	2
CGGTCTA	85	0.0	45.000004	31
GCTAATG	55	1.8189894E-12	45.000004	1
ACGAGAT	55	1.8189894E-12	45.000004	5
AACCGTG	25	3.8888327E-5	45.0	29
AGCCGTT	40	6.8066583E-9	45.0	45
ATCTCGT	25	3.8888327E-5	45.0	19
CTCACGT	80	0.0	45.0	45
GGTACGA	20	7.030906E-4	45.0	2
CTACTTA	20	7.030906E-4	45.0	21
CTCCGAT	25	3.8888327E-5	45.0	28
TCCGATA	25	3.8888327E-5	45.0	29
CGTGCCG	25	3.8888327E-5	45.0	41
>>END_MODULE