FastQCFastQC Report
Thu 26 May 2016
SRR1546909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546909_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2396268
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT388451.6210624187277884No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218500.911834569422118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG253.8920763E-545.0000041
CGTTTTT317550.044.3623051
GTTTTTA217150.042.2852862
CGTTAGA1550.040.645162
CCGCTCG2300.040.10869219
CGACGGT4050.039.44444328
ACGTATG403.460318E-739.3751
TTATTTT242300.039.0383836
CACGACG4100.038.96341726
TATTTTT242750.038.9289367
CGGTCTA4250.038.64705731
TCACGAC4200.038.03571325
TTTTATT249200.038.0206684
TTTATTT248750.038.008045
GTTGCGA1600.037.968752
TTTTTAT252400.037.5475433
GAGCGAT13850.037.364627
TATGCGA1700.037.0588232
GTTACGA1400.036.9642832
AGACTTA17750.036.6338048
Produced by FastQC (version 0.11.5)