##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546909_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2396268
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.695163896525763	31.0	31.0	34.0	30.0	34.0
2	32.04983541073035	33.0	31.0	34.0	30.0	34.0
3	31.92857894025209	31.0	31.0	34.0	30.0	34.0
4	35.76480760916559	37.0	35.0	37.0	35.0	37.0
5	35.79953327424145	37.0	35.0	37.0	35.0	37.0
6	35.86161940150267	37.0	35.0	37.0	35.0	37.0
7	36.10630280085533	37.0	35.0	37.0	35.0	37.0
8	13.273272438642088	0.0	0.0	36.0	0.0	37.0
9	24.936026354314293	18.0	17.0	39.0	17.0	39.0
10	32.63444781635443	32.0	32.0	38.0	27.0	39.0
11	35.520588682067284	37.0	34.0	37.0	32.0	39.0
12	36.75074323907009	38.0	35.0	39.0	33.0	39.0
13	37.091331603977515	39.0	37.0	39.0	33.0	39.0
14	38.34615410296344	40.0	38.0	41.0	34.0	41.0
15	38.566031846187485	40.0	38.0	41.0	34.0	41.0
16	38.60676769042528	40.0	38.0	41.0	34.0	41.0
17	38.56834627846301	40.0	38.0	41.0	34.0	41.0
18	38.46400903404795	40.0	38.0	41.0	34.0	41.0
19	38.38902117793168	40.0	37.0	41.0	34.0	41.0
20	38.29781143010715	40.0	37.0	41.0	34.0	41.0
21	38.24974376822626	40.0	37.0	41.0	34.0	41.0
22	38.21174927011503	40.0	37.0	41.0	34.0	41.0
23	37.9917000101825	39.0	37.0	41.0	34.0	41.0
24	37.63956034967708	39.0	35.0	41.0	33.0	41.0
25	37.53790018478735	39.0	35.0	41.0	33.0	41.0
26	37.467347141471656	39.0	35.0	41.0	33.0	41.0
27	37.42508392216563	39.0	35.0	41.0	33.0	41.0
28	37.28785135886303	39.0	35.0	41.0	33.0	41.0
29	37.19607698304196	39.0	35.0	41.0	33.0	41.0
30	37.023621314477346	39.0	35.0	41.0	32.0	41.0
31	36.8351670180464	39.0	35.0	41.0	31.0	41.0
32	36.472021910737865	39.0	35.0	41.0	30.0	41.0
33	36.05777567450719	39.0	35.0	41.0	28.0	41.0
34	35.62428743362595	39.0	35.0	41.0	24.0	41.0
35	35.296274456780296	39.0	35.0	41.0	22.0	41.0
36	35.10969390735928	39.0	35.0	41.0	21.0	41.0
37	35.03182949486452	39.0	35.0	41.0	20.0	41.0
38	34.914723645268396	39.0	35.0	41.0	19.0	41.0
39	34.79543773901751	39.0	35.0	41.0	18.0	41.0
40	34.69234492969902	39.0	35.0	41.0	18.0	41.0
41	34.59101402681169	39.0	34.0	40.0	18.0	41.0
42	34.512611277202716	39.0	34.0	40.0	18.0	41.0
43	34.402290561823634	38.0	34.0	40.0	18.0	41.0
44	34.2156340609648	38.0	34.0	40.0	17.0	41.0
45	34.133407865898135	38.0	34.0	40.0	17.0	41.0
46	34.092761327197124	38.0	34.0	40.0	17.0	41.0
47	34.050988453712186	38.0	33.0	40.0	18.0	41.0
48	33.956844142641806	38.0	33.0	40.0	17.0	41.0
49	33.85792115072271	38.0	33.0	40.0	17.0	41.0
50	33.767058609471064	38.0	33.0	40.0	17.0	41.0
51	33.429164434028245	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	7.0
12	8.0
13	13.0
14	27.0
15	44.0
16	155.0
17	381.0
18	1005.0
19	2037.0
20	3970.0
21	6852.0
22	11125.0
23	17885.0
24	29726.0
25	45805.0
26	59792.0
27	61969.0
28	56482.0
29	51168.0
30	52887.0
31	62186.0
32	76899.0
33	102870.0
34	147523.0
35	218340.0
36	245360.0
37	405566.0
38	503450.0
39	232694.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.616720667304325	21.510198358447386	25.325172309608107	23.547908664640183
2	40.19671422395158	22.376795917651947	25.614497209827945	11.811992648568523
3	27.183436911063367	22.510420370342548	38.333608761624326	11.972533956969755
4	24.391220013788107	24.100517972113302	38.10888431511	13.399377698988593
5	21.020854094784056	28.127863828252934	37.36927589067667	13.482006186286341
6	19.41352135904665	36.81804372465851	34.35533921915245	9.41309569714239
7	77.96932563469528	3.4236571201551746	16.896857947441603	1.7101592977079356
8	29.39258046261937	63.48096289730532	6.168759087047024	0.9576975530282923
9	73.77822514009284	4.9004952701450755	18.21494924607765	3.106330343684429
10	34.74419388816276	28.933533310965213	24.56933030862992	11.752942492242104
11	27.473012200638657	23.52286972909541	33.80844713529538	15.195670934970545
12	25.743990238153664	20.851173574908984	36.63192097044237	16.772915216494983
13	21.598502337801946	24.08286552255424	37.36405944577151	16.954572693872304
14	18.04051132844907	26.88109176436025	37.709095977578464	17.369300929612212
15	17.37330715929938	23.94740488125702	41.71273830806905	16.966549651374553
16	20.250572974308383	22.481542131347577	40.27420972946265	16.99367516488139
17	19.986287009633312	22.451203287779165	36.431317365169505	21.131192337418018
18	20.868784292908806	23.60136679202827	37.527355037082664	18.00249387798026
19	22.618713766573688	25.153154822415523	34.323164186977415	17.90496722403337
20	23.78102115456201	24.520128800284443	34.12564871708841	17.573201328065142
21	22.43488624811582	24.723194567552547	36.194240377119755	16.64767880721188
22	21.143419684275717	21.742058901591975	36.46349239734454	20.651029016787774
23	19.645715754665172	25.03630645653992	36.012249047268504	19.30572874152641
24	19.480542243188157	24.630383579799922	37.993204432893144	17.895869744118773
25	20.478802871798983	24.57846952010376	36.104475793191746	18.83825181490551
26	18.85022877240776	26.77388338866938	34.594252395808816	19.78163544311404
27	18.935069032345297	24.898675774162154	36.72514927378741	19.441105919705144
28	18.267531010721672	24.995659917838907	38.29680152637351	18.44000754506591
29	19.07457763488892	24.600169930909228	37.54650982277442	18.778742611427436
30	20.4995434567419	24.172504911804523	36.93309763348674	18.39485399796684
31	23.283080189694978	23.58964022388147	34.040766725591624	19.086512860831927
32	22.894893225632526	24.892791624309137	34.136290264694935	18.076024885363406
33	22.35989463615923	24.71075856289864	33.03553692658751	19.89380987435462
34	19.985786230922418	26.42012496098099	33.87667823465489	19.7174105734417
35	20.098252783077687	26.77559438259827	33.04258956009929	20.08356327422475
36	22.51530296277378	27.88123031313693	30.691767364919116	18.911699359170175
37	20.560012486082524	29.209504112227847	30.83236098800301	19.398122413686618
38	20.366461514321436	29.911178549310847	29.7456711853599	19.976688751007817
39	20.42146370940145	28.092099881983152	30.047056506200477	21.43937990241492
40	21.335760440818806	26.684285730978335	31.086339257545482	20.893614570657373
41	19.676638840062964	26.391121527308297	31.21787713227402	22.714362500354717
42	20.808482189805147	27.77819509337019	29.617388372252186	21.795934344572476
43	21.00612285437188	27.292314549123887	29.68440925639369	22.017153340110536
44	21.130733290266367	27.671028449238566	30.91344540760883	20.284792852886238
45	20.513356602850767	28.124650498191357	30.132439276408146	21.229553622549734
46	21.71530897211831	27.60137847686486	30.461325694788734	20.221986856228103
47	20.633585225024913	27.36054564848339	31.237240575761977	20.768628550729716
48	20.595150458963687	26.779058102015302	32.090901351601744	20.53489008741927
49	20.71583812828949	25.927942951289257	32.3406230021016	21.015595918319654
50	19.459050490178896	26.95892112234525	32.76419832840066	20.817830059075195
51	19.615919421366893	27.42986176838317	31.850485838812688	21.103732971437253
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1628.0
1	3286.0
2	4944.0
3	52027.0
4	99110.0
5	66867.0
6	34624.0
7	33690.0
8	32756.0
9	32939.0
10	33122.0
11	32655.0
12	32188.0
13	30631.5
14	29075.0
15	27443.0
16	25811.0
17	24432.0
18	23053.0
19	21770.5
20	20488.0
21	20012.5
22	19537.0
23	20316.0
24	21095.0
25	22671.0
26	27329.0
27	30411.0
28	34121.0
29	37831.0
30	41643.5
31	45456.0
32	51414.0
33	57372.0
34	62793.5
35	68215.0
36	74859.0
37	81503.0
38	93123.5
39	104744.0
40	117537.5
41	130331.0
42	141901.0
43	153471.0
44	161945.0
45	170419.0
46	170420.5
47	170422.0
48	164676.0
49	158930.0
50	149984.0
51	141038.0
52	133469.0
53	125900.0
54	118959.0
55	112018.0
56	106509.0
57	101000.0
58	93733.0
59	86466.0
60	77745.0
61	69024.0
62	60125.5
63	51227.0
64	42377.5
65	33528.0
66	27932.0
67	22336.0
68	19250.0
69	16164.0
70	14005.0
71	11846.0
72	9662.5
73	7479.0
74	5676.5
75	2889.5
76	1905.0
77	1431.0
78	957.0
79	707.0
80	457.0
81	302.5
82	148.0
83	103.0
84	58.0
85	42.5
86	27.0
87	19.5
88	12.0
89	9.5
90	7.0
91	6.0
92	5.0
93	5.5
94	6.0
95	3.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2396268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.277671362483783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84606622940375	22.15899976848051
2	7.517031364262435	4.702304732657671
3	3.86933440093499	3.63071309351993
4	2.6114157089694565	3.26716009343897
5	1.997151542714766	3.1233124807054966
6	1.6091187421141844	3.0197692319436453
7	1.375567797640032	3.0117190257980355
8	1.1697118138326348	2.9268689361497566
9	0.9979968156955885	2.809351477991872
>10	7.871444558599462	44.130232344996486
>50	0.10608567322553004	2.1045325219669184
>100	0.02488455451297059	1.6552429198008474
>500	0.0032444888471989185	0.6614808310540901
>1k	6.759351764997701E-4	0.231924211092538
>5k	0.0	0.0
>10k+	2.70374070599908E-4	2.566388330403208
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38845	1.6210624187277884	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21850	0.911834569422118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2519467772386061E-4	0.0	0.0	0.009765184862461126	0.0
2	1.2519467772386061E-4	0.0	0.0	0.03605606718447185	0.0
3	1.2519467772386061E-4	0.0	0.0	0.062346949506482584	0.0
4	1.2519467772386061E-4	0.0	0.0	0.10203366234494639	0.0
5	1.2519467772386061E-4	0.0	0.0	0.21057744793153355	0.0
6	1.2519467772386061E-4	0.0	0.0	0.3825532035648767	0.0
7	1.2519467772386061E-4	0.0	0.0	0.5058282295636382	0.0
8	1.2519467772386061E-4	0.0	0.0	0.6969587708887319	0.0
9	1.2519467772386061E-4	0.0	0.0	1.0212964493120136	0.0
10	1.2519467772386061E-4	0.0	0.0	1.4522165300375418	0.0
11	1.2519467772386061E-4	0.0	0.0	1.7185890726746758	0.0
12	1.2519467772386061E-4	0.0	0.0	1.9488638165680967	0.0
13	1.2519467772386061E-4	0.0	0.0	2.0655035246474935	0.0
14	1.2519467772386061E-4	0.0	0.0	2.1285181791018366	0.0
15	1.2519467772386061E-4	0.0	0.0	2.1836038373003355	0.0
16	1.2519467772386061E-4	0.0	0.0	2.2626016789440913	0.0
17	1.2519467772386061E-4	0.0	0.0	2.352867041582995	0.0
18	1.2519467772386061E-4	0.0	0.0	2.499219619842188	0.0
19	1.2519467772386061E-4	0.0	0.0	2.5679932294718286	0.0
20	1.2519467772386061E-4	0.0	0.0	2.6478257023004104	0.0
21	1.2519467772386061E-4	0.0	0.0	2.7380910649393138	0.0
22	1.2519467772386061E-4	0.0	0.0	2.8401247272842602	0.0
23	1.2519467772386061E-4	0.0	0.0	2.9509637486291185	0.0
24	1.2519467772386061E-4	0.0	0.0	3.040102359168507	0.0
25	1.2519467772386061E-4	0.0	0.0	3.123189893617909	0.0
26	1.2519467772386061E-4	0.0	0.0	3.202605050854078	0.0
27	1.2519467772386061E-4	0.0	0.0	3.2903665199385044	0.0
28	1.2519467772386061E-4	0.0	0.0	3.383720017961263	0.0
29	1.2519467772386061E-4	0.0	0.0	3.4847103913251773	0.0
30	1.6692623696514748E-4	0.0	0.0	3.607568101731526	0.0
31	1.6692623696514748E-4	0.0	0.0	3.7119804629532256	0.0
32	1.6692623696514748E-4	0.0	0.0	3.8201069329474	0.0
33	1.6692623696514748E-4	0.0	0.0	3.925896435624062	0.0
34	1.6692623696514748E-4	0.0	0.0	4.038571645575536	0.0
35	1.6692623696514748E-4	0.0	0.0	4.171236272403587	0.0
36	1.6692623696514748E-4	0.0	0.0	4.289837363767325	0.0
37	1.6692623696514748E-4	0.0	0.0	4.414906846813461	0.0
38	1.6692623696514748E-4	0.0	0.0	4.56013267297314	0.0
39	1.6692623696514748E-4	0.0	0.0	4.749468757250859	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	25	3.8920763E-5	45.000004	1
CGTTTTT	31755	0.0	44.362305	1
GTTTTTA	21715	0.0	42.285286	2
CGTTAGA	155	0.0	40.64516	2
CCGCTCG	230	0.0	40.108692	19
CGACGGT	405	0.0	39.444443	28
ACGTATG	40	3.460318E-7	39.375	1
TTATTTT	24230	0.0	39.038383	6
CACGACG	410	0.0	38.963417	26
TATTTTT	24275	0.0	38.928936	7
CGGTCTA	425	0.0	38.647057	31
TCACGAC	420	0.0	38.035713	25
TTTTATT	24920	0.0	38.020668	4
TTTATTT	24875	0.0	38.00804	5
GTTGCGA	160	0.0	37.96875	2
TTTTTAT	25240	0.0	37.547543	3
GAGCGAT	1385	0.0	37.36462	7
TATGCGA	170	0.0	37.058823	2
GTTACGA	140	0.0	36.964283	2
AGACTTA	1775	0.0	36.633804	8
>>END_MODULE