FastQCFastQC Report
Thu 26 May 2016
SRR1546908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546908_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences551721
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88071.5962778288301516No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49490.897011351752063No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC253.88816E-545.031
TCGCAAA207.0300954E-445.014
CGTATGA207.0300954E-445.02
TCGATTA207.0300954E-445.041
CGTTAGC207.0300954E-445.027
AACGGCG207.0300954E-445.014
CATACGG207.0300954E-445.02
CGAGAAT351.2103919E-745.06
ATAACGC207.0300954E-445.011
ATCGGTA253.88816E-545.027
TGATGCG406.8048394E-945.01
TACGTAG207.0300954E-445.01
AAATGCG253.88816E-545.01
ACGGTAG207.0300954E-445.01
CGTTTTT67000.044.05971
GTTTTTA45950.042.6006552
TTACGAG800.042.18753
AGCGATA603.6379788E-1241.2499968
TTAAGCA1750.041.14285745
TATTTTT50100.040.014977