FastQCFastQC Report
Thu 26 May 2016
SRR1546907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546907_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1651105
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442572.6804473367835477No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250351.5162572943574153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.034181E-445.0000046
CGTTTTT265850.044.280611
GTTTTTA176400.043.4693872
TCACGAC4800.042.65625425
CACGACG4900.041.78571326
TAGCCGT2100.041.78571344
GGTACGA650.041.538462
CGTTAGA1300.041.538462
CGCGTCA603.6379788E-1241.25000439
CGGTCTA5050.040.990131
CGACGGT5150.040.63106528
TTATTTT191750.040.6114736
CGAGAAT2050.040.6097566
AGACTTA15600.040.5288478
CCGCTCG2950.040.4237319
TATTTTT193250.040.272967
CGCATCG1850.040.13513621
TTTTATT196000.039.8456654
TTTATTT196100.039.745035
AGCGATA2050.039.5121968
Produced by FastQC (version 0.11.5)